BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1121.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr... 426 e-121 gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro... 149 2e-37 gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily pro... 76 3e-15 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 69 4e-13 gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum se... 62 4e-11 >gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydrolase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 426 bits (1096), Expect = e-121, Method: Compositional matrix adjust. Identities = 222/258 (86%), Positives = 240/258 (93%) Query: 1 MPFATLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWEPQIAELSQHFRVLRYDTRGHGQ 60 MPFA +N I+LHYQL+GDA+LPVLVLSNSLGTSL+MW+PQ+ + QHFRVLRYDTRGHGQ Sbjct: 1 MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQ 60 Query: 61 SEVTPGPYTIAQLGSDVIALLDQLEIAQAHFCGLSMGGSTFMWLAVHHPERVNKLILCNT 120 SEVTPG +IAQLGSDVIALLD L IAQAHFCGLSMGGSTFMWLAVHHP+R+NKLILCNT Sbjct: 61 SEVTPGECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNT 120 Query: 121 GAKIGTAEAWNSRIDTVLRDGLPAIAPAVVSRWLTPEYAEHNPQQVQALTAMLLATPAEG 180 GAKIGTAEAWNSRI+TV R+GL AIA AVVSRWLTPEYA+ +PQQVQALTAMLLATPAEG Sbjct: 121 GAKIGTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEG 180 Query: 181 YAGACAAVRDNDLREAIAGIRAPTLVIAGSGDLPTPPADAQFMRATIPGALYVEFDAAHI 240 YA ACAAVRD+DLREAIAGIRAPTL+IAGSGD+PTPPADAQFMRATIPGA YVEFDAAHI Sbjct: 181 YAAACAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQYVEFDAAHI 240 Query: 241 SNQQQPRAFTQAVRQFLT 258 SNQQQ AFTQAV QFLT Sbjct: 241 SNQQQAAAFTQAVVQFLT 258 >gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 149 bits (377), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 101/254 (39%), Positives = 131/254 (51%), Gaps = 7/254 (2%) Query: 11 LHYQL----DGDAALPVLVLSNSLGTSLAMWEPQIAELSQHFRVLRYDTRGHGQSEVTPG 66 LHY + +G A +VLS++LGT L MW+ +L+ RV+ YD RGHG SE G Sbjct: 60 LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADG 119 Query: 67 PYTIAQLGSDVIALLDQLEIAQAHFCGLSMGGSTFMWLAVHHPERVNKLILCNTGAKIGT 126 Y++A L D LL +L+ + GLSMGG LA+ HP V L+L NT + Sbjct: 120 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPD 179 Query: 127 A--EAWNSRIDTVLRDGLPAIAPAVVSRWLTPEYAEHNPQQVQALTAMLLATPAEGYAGA 184 A EAW RI TV +G+ IA AV+ R+ + V L+ T A GY G Sbjct: 180 AAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGC 239 Query: 185 CAAVRDNDLREAIAGIRAPTLVIAGSGDLPTPPADAQFMRATIPGA-LYVEFDAAHISNQ 243 C AV D + IR PTLVIAG D TP A AQ + I GA L V A+H+S Sbjct: 240 CHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAV 299 Query: 244 QQPRAFTQAVRQFL 257 +QP F + V F+ Sbjct: 300 EQPALFAELVCGFI 313 >gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 75.9 bits (185), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 50/143 (34%), Positives = 71/143 (49%), Gaps = 13/143 (9%) Query: 1 MPFATLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWE--PQIAELSQHFRVLRYDTRGH 58 MPFA + K+HYQ++G+ P L+L + +SL W L QH+RV+ +D GH Sbjct: 1 MPFAISDQRKIHYQVEGEG--PPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGH 58 Query: 59 GQSEV--TPGPYTIAQLGSDVIALLDQLEIAQAHFCGLSMGGSTFMWLAVHHPERVNKLI 116 GQS+ Y+ Q D +A+LD ++ + HF G S+GG L PER+ LI Sbjct: 59 GQSDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLI 118 Query: 117 LCNTGAKIGTAEAWNSRIDTVLR 139 L G A + R V R Sbjct: 119 L-------GAAHPYEDRSWEVFR 134 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 68.9 bits (167), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 87/284 (30%), Positives = 115/284 (40%), Gaps = 42/284 (14%) Query: 5 TLNGIKLHYQLDGD-AALPVLVLSNSLGTSLAMWEPQIAELSQH----FRVLRYDTRGHG 59 TL+ + H GD P+L+L G + +L+Q +R + D RG G Sbjct: 9 TLSALNFHITEWGDPQGFPLLLLHGIRG-----YGETFGDLAQRLPPGYRCIALDQRGRG 63 Query: 60 QSEVTPGP--YTIAQLGSDVIALLDQLEIAQAHFCGLSMGGSTFMWLAVHHPERVNKLIL 117 QS+ G YT A + +DV AL+ +L + Q G SMGG + A HP +V +LI+ Sbjct: 64 QSDWDAGANYYTDAYV-ADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLII 122 Query: 118 CNTGAKIGTAEAWNSRIDTVLRDGLPAIAPAVVSRW---------LTPEYAEHNPQQVQA 168 + G G E NS T +R L A P+ + W L P E Q Q Sbjct: 123 EDAGP--GAFE--NSPGATRIRREL-ATTPSHFASWDEASTFMRALRPSVTEEARQ--QR 175 Query: 169 LTAMLLATPAEGYAGACAAV------------RDNDLREAIAGIRAPTLVIAGSGDLPTP 216 L ML GY V R DLR I + PTLV+ G Sbjct: 176 LHNMLKPAAQGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLS 235 Query: 217 PADAQFMRATIPGALYVEF-DAAHISNQQQPRAFTQAVRQFLTE 259 A MR L DA H + QP AF + VRQFL + Sbjct: 236 AEMAAEMRQLNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQ 279 >gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 62.0 bits (149), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 61/242 (25%), Positives = 101/242 (41%), Gaps = 18/242 (7%) Query: 5 TLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWEPQIAELSQH-FRVLRYDTRGHGQSEV 63 T +G +++Y+ D + PV V + MWE Q+ L++ +RV+ YD RG G+S Sbjct: 6 TRDGTEIYYK-DWGSGQPV-VFGHGWPLDGDMWEYQMNFLAERGYRVIAYDRRGFGRSSQ 63 Query: 64 TPGPYTIAQLGSDVIALLDQLEIAQAHFCGLSMGGSTF-MWLAVHHPERVNKLILCNTGA 122 Y D+ L++ L++ A G SMGG ++ H +RV K L Sbjct: 64 PWSGYDYDTFADDLAELMEHLDLQGATLVGFSMGGGDVARYIGRHGSKRVAKAALLGAVT 123 Query: 123 KI-GTA----EAWNSRIDTVLRDGLPAIAPAVVSRWLTPEYAEHNPQQVQALTAMLLA-- 175 I G A + + + +++GL +S + + + P + A++ +LA Sbjct: 124 PIFGQAPDFPQGVPAEVFAGIKEGLLKDRAQFISDFAAVFFGTNRPGKADAVSPGVLAQT 183 Query: 176 ------TPAEGYAGACAAVRDNDLREAIAGIRAPTLVIAGSGDLPTP-PADAQFMRATIP 228 +G A D R I PTLVI G D P + + + +P Sbjct: 184 FNIAMLASLKGTLDCVTAFSGTDFRADIKKFNFPTLVIHGDDDQIVPLQSTGKLVAEMVP 243 Query: 229 GA 230 GA Sbjct: 244 GA 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 912,457 Number of extensions: 39996 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 6 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)