BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1098.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 208 7e-55 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 173 2e-44 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 172 3e-44 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 172 4e-44 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 158 8e-40 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 208 bits (529), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 124/384 (32%), Positives = 214/384 (55%), Gaps = 11/384 (2%) Query: 11 STQSK---KRLRKVAAATIVGSMLEWYDFYLYATMASIVFSKIFFDVSEPATASLQAFAT 67 STQS ++ +K AA+ +GS LE+YDF++YAT AS++F ++FF +P TA + + AT Sbjct: 3 STQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLAT 62 Query: 68 FAIGFVARPIGGLFFGYFGDRYGRKRMLFVTFCMMGLCTTAIGLLPSYAVIGVWSPLCLV 127 + +G+VARPIG G+ GD +GRK++L +MG T +GLLP+Y +G+W+P LV Sbjct: 63 YGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLV 122 Query: 128 LIRIVQGLGAGAELAGAAVTSYEHAAENKKGRQGAWPALGLNLGLLLSSLTVYLLT--VN 185 ++R+ QG E++GA+ EH+ ++G ++ G+ G +L++ L + Sbjct: 123 IMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMP 182 Query: 186 GEQFLLAGGWRIPFLLSFFLVLIGLWVRRSLPETPAYQHAGETHRHKR-PFRELWNTNVK 244 EQF + GWRIPFLLSF +++ G +RR++ ETP ++ A + +R P + + Sbjct: 183 AEQFNV-WGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSP 241 Query: 245 GLAVVFFVAVGYNALSYIFKTFSLAYLTQFK---GISASVTSLSVTLASLVAIFSVPLFG 301 + V +A+ N + + F AY Q G + V + ++VA+ +P G Sbjct: 242 NMLRVVCMAL-MNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 Query: 302 YLCDRFSSKKVLIAGGVLSGLFAYPFMHLLATGDNVHIYLALIIGTGVLAPMMFAPQGSF 361 L DR + +I G +L+GLFA+ +++ ++ + ++ I+ GV+ A SF Sbjct: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 Query: 362 LSRQFTVQSRSTGVGSAREIGTAL 385 F ++R + + A+ +GTA+ Sbjct: 361 YPELFPARARVSAMAIAQNLGTAI 384 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 173 bits (439), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 110/397 (27%), Positives = 193/397 (48%), Gaps = 4/397 (1%) Query: 6 PEHETSTQSKKRLRKVAAATIVGSMLEWYDFYLYATMASIVFSKIFFDVSEPATASLQAF 65 P + S ++ R+ AT++G+ LEW+DF +Y+ A I+ +K+FF T+ L A Sbjct: 7 PADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVII-AKLFFPTGNDLTSLLLAV 65 Query: 66 ATFAIGFVARPIGGLFFGYFGDRYGRKRMLFVTFCMMGLCTTAIGLLPSYAVIGVWSPLC 125 ATF +GF RP+GG+ G + DR GRK L VT +M + TT IGL P+Y IG+++PL Sbjct: 66 ATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLI 125 Query: 126 LVLIRIVQGLGAGAELAGAAVTSYEHAAENKKGRQGAWPALGLNLGLLL-SSLTVYLLTV 184 +V+ R++QG AG E+ A E+A E ++ +W + + +LL +++ ++ Sbjct: 126 IVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTAS 185 Query: 185 NGEQFLLAGGWRIPFLLSFFLVLIGLWVRRSLPETPAYQHAGETHRHKRPFRELWNTNVK 244 L + GWR+PFLL + +G ++R L ETP + E + P +E+ + Sbjct: 186 LDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLE--EKDKTDSPLKEVIRNFPR 243 Query: 245 GLAVVFFVAVGYNALSYIFKTFSLAYLTQFKGISASVTSLSVTLASLVAIFSVPLFGYLC 304 + F + + + +Y+ + Y + + + ++ + L + P+ G L Sbjct: 244 ETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLA 303 Query: 305 DRFSSKKVLIAGGVLSGLFAYPFMHLLATGDNVHIYLALIIGTGVLAPMMFAPQGSFLSR 364 DR + +L L + AYP + + ++ L + G L P + S Sbjct: 304 DRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSE 363 Query: 365 QFTVQSRSTGVGSAREIGTALAGGIAPLWALSLVARS 401 F + STG+ A + + GG A + L+A + Sbjct: 364 LFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAAT 400 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 172 bits (437), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 115/314 (36%), Positives = 174/314 (55%), Gaps = 8/314 (2%) Query: 19 RKVAAATIVGSMLEWYDFYLYATMASIVFSKIFFDVSEPATASLQAFATFAIGFVARPIG 78 +KV A+ +G++ EWYDFYLY ++A+I+ +K FF ++P TA + A FA GF+ RP G Sbjct: 18 KKVIFASSLGTVFEWYDFYLYGSLAAII-AKQFFAGTDPNTAFIFALLAFAAGFIVRPFG 76 Query: 79 GLFFGYFGDRYGRKRMLFVTFCMMGLCTTAIGLLPSYAVIGVWSPLCLVLIRIVQGLGAG 138 L FG GD GRK VT +MG T +G+LP+Y IG+ +P+ L+++RI+QGL G Sbjct: 77 ALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQGLALG 136 Query: 139 AELAGAAVTSYEHAAENKKGRQGAWPALGLNLGLLLSSLTVY-LLTVNGEQFLLAGGWRI 197 E GAA EHA E ++G AW +GL LS L + + T GE GWRI Sbjct: 137 GEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAAFADWGWRI 196 Query: 198 PFLLSFFLVLIGLWVRRSLPETPAY-QHAGETHRHKRPFRELWNTNVKGLAVVFFVAVGY 256 PFL+S L+ I +W+R S+ E+PA+ + E K P E + K L +V VG Sbjct: 197 PFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG-QWKNLKLVILALVGL 255 Query: 257 NA----LSYIFKTFSLAYLTQFKGISASVTSLSVTLASLVAIFSVPLFGYLCDRFSSKKV 312 A + Y + ++L +LTQ + ++ + A L+A FG L D+ K + Sbjct: 256 TAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFFLFFGSLSDKIGRKPI 315 Query: 313 LIAGGVLSGLFAYP 326 ++ G +++ + +P Sbjct: 316 ILGGCLIAAVTYFP 329 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 172 bits (436), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 122/393 (31%), Positives = 192/393 (48%), Gaps = 34/393 (8%) Query: 16 KRLRKVAAATIVGSMLEWYDFYLYATMASIVFSKIFFDVSEPATASLQAFATFAIGFVAR 75 + R+V AA IVG+ LEWYDF +Y ++I+ +++FF T+ L A ATF IGF R Sbjct: 6 NKQRQVIAA-IVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFFMR 63 Query: 76 PIGGLFFGYFGDRYGRKRMLFVTFCMMGLCTTAIGLLPSYAVIGVWSPLCLVLIRIVQGL 135 P+GG+ G + DR GRK + V +M L I P+YA IG+ +P+ +V+ R++QG Sbjct: 64 PVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGF 123 Query: 136 GAGAELAGAAVTSYEHAAENKKGRQGAWPALGLNLGLLLSSLTVYLLTVN-GEQFLLAGG 194 G E + + E A +K+G G+W +G L + + L+T N + L + G Sbjct: 124 ATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWG 183 Query: 195 WRIPFLLSFFLVLIGLWVRRSLPETPAY-QHAGETHRHKRPFRELWNTNVKGLAV----- 248 WR+PF+L + +GLW+RR + ET + + A + + ++ TN++G+ V Sbjct: 184 WRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQV 243 Query: 249 ----VFFVAVGYNALSYIFKTFSLAYLTQFKGISASVTSLSVTLASLVAIFSVPLFGYLC 304 V F V N ++ K L F V ++V L + V +P G L Sbjct: 244 INGTVAFYVVLVNMPTFANKQLGLPLDQAFM-----VQMIAVALLTAV----IPFAGALS 294 Query: 305 DRFSSKKVLIAGGVLSGLFAYPFMHLLATGDN------VHIYLALIIGTGVLAPMMFAPQ 358 DR + VL+ + L YP ++ + + + L ++IG F P Sbjct: 295 DRLGRRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGAN------FGPM 348 Query: 359 GSFLSRQFTVQSRSTGVGSAREIGTALAGGIAP 391 + LS QF + RSTG+ A + L GG AP Sbjct: 349 PTALSEQFPTRVRSTGLAVAYNLAAMLFGGFAP 381 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 158 bits (400), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 120/401 (29%), Positives = 194/401 (48%), Gaps = 25/401 (6%) Query: 12 TQSKKRLRKVAAATIVGSMLEWYDFYLYATMASIVFSKIFFDVSEPATASLQAFATFAIG 71 T K + R V AAT+ G++LE YDF ++ A + SK FF + A + F TFA G Sbjct: 8 TDPKTQRRAVIAATL-GNVLEIYDFIIFGIFA-VAISKTFFPSGDEYAALMFTFMTFAAG 65 Query: 72 FVARPIGGLFFGYFGDRYGRKRMLFVTFCMMGLCTTAIGLLPSYAVIGVWSPLCLVLIRI 131 FV RPIG L G + D+ GRK+ L T +M + T L PSY IG+ + + LV R+ Sbjct: 66 FVVRPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFGRL 125 Query: 132 VQGLGAGAELAGAAVTSYEHAAENKKGRQGAWPALGLNLGLLLSSLTVYLL-TVNGEQFL 190 +QG AG E+ E+A+ + ++ + G+LL + Y++ V G++ + Sbjct: 126 LQGFSAGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIAVVYGQERM 185 Query: 191 LAGGWRIPFLLSFFLVLIGLWVRRSLPETPAYQHAGETHRHKRPFRELWNTN----VKGL 246 L WR+ F + + +G ++RR LPETP ++ + + F E N + G+ Sbjct: 186 LQDYWRLAFFVGLVIGPVGWYIRRVLPETPEFERSKKKESVDS-FLEGVKANFGRILIGI 244 Query: 247 AVVFFVAVGYNALSYIFKTFSLAYL------TQFKGISASVTSLSVTLASLVAIFSVPLF 300 A++ F V +Y F T+++ L T IS+ +T L++ PL Sbjct: 245 AIMIFWTVATQVSNY-FTTYAVRELHMPVASTYLGQISSGLTLLAIC----------PLV 293 Query: 301 GYLCDRFSSKKVLIAGGVLSGLFAYPFMHLLATGDNVHIYLALIIGTGVLAPMMFAPQGS 360 G L DR K + G L+G+ AYP +L + L + I V + A Sbjct: 294 GMLADRIGPYKPMAFGAALAGVLAYPLFKMLVATPTLPTLLLVQIAISVTLGLYAACAPL 353 Query: 361 FLSRQFTVQSRSTGVGSAREIGTALAGGIAPLWALSLVARS 401 +S F + R+TG+G++ +G + GG+ P+ +L+ S Sbjct: 354 VMSDIFPTRYRATGIGTSYALGVMIFGGLTPMIVTTLIQIS 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,221,182 Number of extensions: 48147 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 16 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)