BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1095.p (152 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312216|ref|YP_003776308.1| IS1404 transposase [Herbaspiril... 110 4e-26 >gi|300312216|ref|YP_003776308.1| IS1404 transposase [Herbaspirillum seropedicae SmR1] Length = 367 Score = 110 bits (276), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 52/126 (41%), Positives = 77/126 (61%) Query: 1 MDFMHDQLADGRSIRLFNVIDDFNREALDIEVDLSLPSERVIRSRKQIIEWRGKPSAIRC 60 MDF+HD+LADGR IR N++DDF +E++ IEVD S+ R+ R ++ + R P+ IR Sbjct: 208 MDFVHDRLADGRRIRCLNIVDDFTKESVAIEVDTSISGLRLGRVLDKVAQERPLPTMIRV 267 Query: 61 DNGPEYLSGAITQWAQQTGIERQYIQPGQPQQNAYLERLNRTVRYEWLSRYQWDDLEHVQ 120 D+GPE+ S A+ WA G++ + QPG+P QNAY+E N R E L+ + + L + Sbjct: 268 DHGPEFTSLALDAWAAGRGVKLAFTQPGKPTQNAYIESFNGRFRDECLNDHLFSTLHEAR 327 Query: 121 QFATNW 126 W Sbjct: 328 YRIEAW 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 520,991 Number of extensions: 20726 Number of successful extensions: 48 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 48 Number of HSP's successfully gapped: 1 Length of query: 152 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 75 Effective length of database: 1,255,275 Effective search space: 94145625 Effective search space used: 94145625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)