BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1084.p (454 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 858 0.0 gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 201 1e-52 gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large ... 141 1e-34 gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 63 4e-11 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 858 bits (2216), Expect = 0.0, Method: Compositional matrix adjust. Identities = 395/449 (87%), Positives = 427/449 (95%) Query: 1 MSTIAPIDKYNEVETLLETALEDNKEKGVFRCRRDIFTNPELFELEMKHIFESNWVYLAH 60 M+T A D+Y+E++TLL++AL+++K+ GVFRCRRDIFTNP+LFELEMKH+FESNWVYLAH Sbjct: 1 MNTTAAADRYDELDTLLQSALQEDKDNGVFRCRRDIFTNPDLFELEMKHLFESNWVYLAH 60 Query: 61 ESQIPNNNDYYTTWIGRQPVVITRDKNGELNALINACAHKGAMLCRRKHGNKSSFTCPFH 120 ESQIP NDYYTTWIGRQPVVITRDK+G+LNA+INAC+HKGAMLCRRKHGNKSSFTCPFH Sbjct: 61 ESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVINACSHKGAMLCRRKHGNKSSFTCPFH 120 Query: 121 GWTFSNSGKLLKVKDEKNTEYPVSFNKDGSHDLTKVARFKSYRGFLFGSLNPDVLPLEEY 180 GWTFSNSGKLLKVKDEK TEYPVSFNK+GSHDLT+VARF+SYRGFLFGSL+ DVLPLEEY Sbjct: 121 GWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY 180 Query: 181 LGETRVIIDQIVDQAPNGLEVLRGNSSYIYDGNWKMQMENGCDGYHVSTVHWNYAATMGR 240 LGETRVIIDQIVDQAPNGLEVLRGNSSY+YDGNWKMQMENGCDGYHVS+VHWNYAATM R Sbjct: 181 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMSR 240 Query: 241 RKEGGTRATDANNWSKAVAGVYGYENGHILLWTNTLNPEVRPIWNQREELAKRVGQERAD 300 RKEGGT+ATDANNWSKAVAGVYG+ENGHILLWTNTLNPEVRPIWNQREELA RVGQ RAD Sbjct: 241 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD 300 Query: 301 IIVSQTRNLCIYPNVFLMDQFGTQIRVARPIGVDKTEISIFCFGPKGESAADRTTRIRQY 360 IV+QTRNLC+YPNVFLMDQFGTQIRVARPI VDKTEISIFCF PKGES DR RIRQY Sbjct: 301 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQY 360 Query: 361 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWIEGPDENAKKMGMKPLLS 420 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLW EGPDENA+KMGM P LS Sbjct: 361 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGMHPKLS 420 Query: 421 GERSEDEGLFICQHHYWTKAMRKAIKEER 449 GERSEDEGLF+CQHHYWT+ MRKA+K+ER Sbjct: 421 GERSEDEGLFVCQHHYWTEQMRKAVKKER 449 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 201 bits (510), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 137/437 (31%), Positives = 209/437 (47%), Gaps = 66/437 (15%) Query: 36 IFTNPELFELEMKHIFESNWVYLAHESQIPNNNDYYTTWIGRQPVVITRDKNGELNALIN 95 ++T+P LFE EM IF W+++AHES+I N D+ T IGRQPV++ RDK G++N L N Sbjct: 29 LYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQPVIVVRDKTGKINVLEN 88 Query: 96 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKNTEYPVSFNKDGSHDLTK 155 C H+GA +C + GN + FTCP+H W++ GKL + YP +G D+ + Sbjct: 89 RCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKL------RGLPYP-----EGYEDVIE 137 Query: 156 VA-------RFKSYRGFLFGSLNPDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSY 208 A R +SY G +F S N ++ PL ++LG + ID + Q ++G + Sbjct: 138 KADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQGEHKF 197 Query: 209 IYDGNWKMQMENGCDGYHVSTVHWNYAATMGRR-KEGGTRATDANNWSKAVAGVYGYENG 267 + GNWK+Q+EN DGYH VH + +++ E + TD + ++++ NG Sbjct: 198 SFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTDDQSITRSLG------NG 251 Query: 268 H--ILLWTNTLNPEVRPIWNQ--------REELAKRVGQERADIIVSQTR----NLCIYP 313 H +++ ++ +V Q EEL+K + E+ IV NL ++P Sbjct: 252 HSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSLHGAGFNLNLFP 311 Query: 314 NVFLMDQFGTQIRVARPIGVDKTEISIFCFGPKGESAADRTTRIRQYEDFFNVSGMGTAD 373 NV + F RV P+ V +T+I G G R+R +E F G G+ D Sbjct: 312 NVAMSMSF---FRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQGPFGFGSPD 368 Query: 374 DLEEFRACQAG-YAGSAVRWNDMSRGAPLWI------EGPDENAKKMGMKPLLSGERSED 426 D E + QAG +AG R AP+ + E D+N K+ S D Sbjct: 369 DAEAWERVQAGSHAG---------RDAPILVNRGLNREWVDDNGDKVS--------HSTD 411 Query: 427 EGLFICQHHYWTKAMRK 443 E + W K M K Sbjct: 412 ETGMREAYAMWKKMMEK 428 >gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large subunit [Herbaspirillum seropedicae SmR1] Length = 426 Score = 141 bits (355), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 113/395 (28%), Positives = 177/395 (44%), Gaps = 49/395 (12%) Query: 31 RCRRDIFTNPELFELEMKHIFE-SNWVYLAHESQIPNNNDYYTTWIGRQPVVITRDKNGE 89 R +F++ E+++LE + I+ W +LA E++IPNN DY +T++G PVV+TR ++G Sbjct: 25 RTPYKVFSSREVYDLEQERIYRGETWSFLALEAEIPNNGDYKSTFVGDTPVVVTRTEDGA 84 Query: 90 LNALINACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKNTEYPVSFNKD- 148 L A +N C+H+GAM+CR GN SS +C +H W+F + G LL V + + KD Sbjct: 85 LAAWVNKCSHRGAMVCRTPRGNASSHSCAYHQWSFDSRGNLLGVPFRRGQKGATGMAKDF 144 Query: 149 --GSHDLTKVARFKSYRGFLFGSLNPDVLPLEEYLG-ETRVIIDQIVDQAPNGLEVLRGN 205 H L ++ R SY+G +F + + V L +Y+G E R ID+I + L R Sbjct: 145 DPKCHGLRQL-RVDSYKGLVFATFSETVEALPDYIGAEMRPWIDRIFHKPIVYLGCTRQY 203 Query: 206 SSYIYDGNWKMQMENGCDGYHVSTVHWNY-----------AATMGRRKEGGTRATDANNW 254 S NWK+ +EN D YH S +H + A ++ + G A Sbjct: 204 SK----SNWKLYLENVKDPYHASLLHLFHTTFNIFRVGMKARSLADKHHGLHSIITATKN 259 Query: 255 SKAVAGVY------GYENGHILLWTNTLNPEVRPIWNQREELAKRVGQERADIIVSQTRN 308 A Y ++ G L +P V + EEL Q Sbjct: 260 EVETADAYKQQEIRSFDEGFSL-----QDPSVLGQIKEFEELTTNHIQP----------- 303 Query: 309 LCIYPNVFLMDQFGTQI-RVARPIGVDKTEISIFCFGPKGESAADRTTRIRQYEDFFNVS 367 I+P + + T + R P G E+ FG + ++ R RI+Q + + Sbjct: 304 --IFPQLVVQQIHNTLVARQLLPKGPSNFELVFHFFGYEDDTPELRALRIKQ-ANLVGPA 360 Query: 368 GMGTADDLEEFRACQAGYAGSAVRWN--DMSRGAP 400 G + +D E Q G A + + DM+R P Sbjct: 361 GYISMEDTEATELVQRGTARDPDQCSVIDMARDNP 395 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 50/204 (24%), Positives = 86/204 (42%), Gaps = 36/204 (17%) Query: 36 IFTNPELFELEMKHIFESNWVYLAHESQIPNNNDYYTTWIGRQPVVITRDKNGELNALIN 95 ++ + L++ E+K +F+ Y+ HE +P DY T + ++ R+ G + + N Sbjct: 21 VYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPWENEGRMLVRNAQG-IELVSN 79 Query: 96 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKNTEYPVSFNKDGSHDLTK 155 C H+ A + + G+ S+ CP H WT+ G+L+ F + +L+K Sbjct: 80 VCRHRQAKMLDGR-GHASNIVCPLHRWTYDLKGELIGAP---------HFGQTPCMNLSK 129 Query: 156 VARFKSYRGFLFGSLNPDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSYIYDG--- 212 +S+ G LF + D+ + LG TR + S Y+YD Sbjct: 130 TP-LQSWNGLLFEANGVDIADKLKNLGVTRDL----------------DFSGYLYDHTEV 172 Query: 213 -----NWKMQMENGCDGYHVSTVH 231 NWK +E + YHV H Sbjct: 173 HECDYNWKTFIEVYLEDYHVEPFH 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,462,403 Number of extensions: 62487 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 4 Length of query: 454 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 366 Effective length of database: 1,203,190 Effective search space: 440367540 Effective search space used: 440367540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)