BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1081.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 239 2e-64 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 205 4e-54 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 180 1e-46 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 179 3e-46 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 168 4e-43 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 239 bits (610), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 119/279 (42%), Positives = 185/279 (66%), Gaps = 1/279 (0%) Query: 1 MVAVPVSSVIGSPLSGLLMEHMNGFHAMHGWQWLFLVEGVPAVGLGILCLFMLKDKVADA 60 M + +S VIG PLSG +M M G GWQW+F++E +P++ LG++ + +KD++ DA Sbjct: 157 MTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDA 216 Query: 61 KWLSSEEKATIKAQLAQDDSKKATHSFSAALASGRLWHLAFLYFCIALGNLGVTFWLPSL 120 WLS EEK ++A ++ ++S+K S A A+GR+W A +YFC +G GV+FWLP++ Sbjct: 217 DWLSEEEKRLLEANISHEESQKEHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTI 276 Query: 121 LRDAGVQSTSTIGWLAAIPFLFSAIVMVLVGRSADARAERRYHLAIPMVVGALGLVLAS- 179 ++ GV +G L AIP+ F+A MVL+GRSADAR ERR+H+AIP +G +GL+L++ Sbjct: 277 IKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQ 336 Query: 180 WMPNLPLLILMLTIATAGLTTALPMFWPLPSAILGGSAMAGGLAVLNSAGALAGFVGPSA 239 + N L + LT+A+ G+ T LP+FW LP+A L G+A A G+A++NS G LAGFV P Sbjct: 337 YGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFL 396 Query: 240 IGYIRMNFAVPSLPLWLMAGIVLIGALTVASIPKSQVNR 278 +G+++ + ++++A ++IG L ++P VNR Sbjct: 397 VGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAKLVNR 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 205 bits (521), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 112/282 (39%), Positives = 178/282 (63%), Gaps = 6/282 (2%) Query: 1 MVAVPVSSVIGSPLSGLLME--HMNGFHAMHGWQWLFLVEGVPAVGLGILCLFMLKDKVA 58 M +PV+ ++G+PLSG +M+ H NG + GWQW+FL+E +PAV +G+ + L + V Sbjct: 158 MSGIPVAGILGNPLSGWIMDAFHQNG--GLEGWQWMFLIEAIPAVLIGVATVLYLDNDVK 215 Query: 59 DAKWLSSEEKATIKAQLAQD-DSKKATHSFSAALASGRLWHLAFLYFCIALGNLGVTFWL 117 AKWL+ EEKA+++A + D K++ H F A + R+W + +YF +G G+T W+ Sbjct: 216 SAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWM 275 Query: 118 PSLLRDAGVQSTSTIGWLAAIPFLFSAIVMVLVGRSADARAERRYHLAIPMVVGALGLVL 177 P+L++ GV+ IG L+AIPF + I M L+GRSAD ERR+HL IP ++G +G V Sbjct: 276 PTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVG 335 Query: 178 AS-WMPNLPLLILMLTIATAGLTTALPMFWPLPSAILGGSAMAGGLAVLNSAGALAGFVG 236 A+ + N + I L++A AG+ T P+FW LP+A L G+A A G+A +NS G LAGFV Sbjct: 336 AALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVS 395 Query: 237 PSAIGYIRMNFAVPSLPLWLMAGIVLIGALTVASIPKSQVNR 278 P IGY++ + ++++A ++++G++ + VN+ Sbjct: 396 PYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPGMVNK 437 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 109/270 (40%), Positives = 172/270 (63%), Gaps = 7/270 (2%) Query: 1 MVAVPVSSVIGSPLSGLLMEHMNGFHAMHGWQWLFLVEGVPAVGLGILCLFMLKDKVADA 60 ++ +PV+++IGSPLSG LME ++G GWQ L L+E +PAV LG++CLF+L D+ A A Sbjct: 160 LIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKA 218 Query: 61 KWLSSEEKATIKAQLAQDDS---KKATHSFSAALASGRLWHLAFLYFCIALGNLGVTFWL 117 KWLS++EK ++ +L+ + + + + A + R++ LA FC +G+L + WL Sbjct: 219 KWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWL 278 Query: 118 PSLLRDAGVQSTSTIGWLAAIPFLFSAIVMVLVGRSADARAERR-YHLAIPMVVGALGLV 176 P ++R+ G+ S +G +AAIP+L A+ M L R A+ R+ERR + +A +V+ AL L Sbjct: 279 PQIIREFGLPS-HQVGLVAAIPYLVGAVAMTLWARLAN-RSERRLFFVAGAIVLAALSLG 336 Query: 177 LASWMPNLPLLILMLTIATAGLTTALPMFWPLPSAILGGSAMAGGLAVLNSAGALAGFVG 236 +++++ L +L +T+A A + + FW +PS L G A AGGLA++ S G L GFVG Sbjct: 337 ISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVG 396 Query: 237 PSAIGYIRMNFAVPSLPLWLMAGIVLIGAL 266 PS IG+IR + PL +AG +L+GA+ Sbjct: 397 PSVIGFIREATQGFTYPLIFVAGALLLGAV 426 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 179 bits (453), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 102/267 (38%), Positives = 160/267 (59%), Gaps = 3/267 (1%) Query: 5 PVSSVIGSPLSGLLMEHMNGFHAMHGWQWLFLVEGVPAVGLGILCLFMLKDKVADAKWLS 64 P S ++G P+SG LM+ M ++ GWQW+F++E +PA LGI+ LF+L + +A AKWL+ Sbjct: 165 PFSYILGGPISGALMQFMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLA 224 Query: 65 SEEKATIKAQLAQDDSKKATHSFSAALASGRLWHLAFLYFCIALGNLGVTFWLPSLLRDA 124 EEKA + +LA ++ +K + A LW LA +Y + +G+ G+ FW P+++RDA Sbjct: 225 PEEKAELARRLAAENKEKVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDA 284 Query: 125 GVQSTSTIGWLAAIPFLFSAIVMVLVGRSAD-ARAERRYHLAIPMVVGALGLVLASWMP- 182 GV+S IG ++AIP++ A MV + R A+ A R Y +A+ MV G L LVL + P Sbjct: 285 GVKSVLDIGMISAIPYILGATAMVFITRHAEKAGKHRSYSVAVSMVAG-LALVLCTLFPH 343 Query: 183 NLPLLILMLTIATAGLTTALPMFWPLPSAILGGSAMAGGLAVLNSAGALAGFVGPSAIGY 242 N+ + + + IA G T++ +FW P A G+ A G+A++NS G L GFVGP +G Sbjct: 344 NIYITLAGIAIAVGGSLTSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGA 403 Query: 243 IRMNFAVPSLPLWLMAGIVLIGALTVA 269 + F P L ++ G + + + +A Sbjct: 404 LTTAFGSPRYGLMMLGGALFLAGVLIA 430 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 168 bits (426), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 101/270 (37%), Positives = 157/270 (58%), Gaps = 2/270 (0%) Query: 1 MVAVPVSSVIGSPLSGLLMEHMNGFHAMHGWQWLFLVEGVPAVGLGILCLFMLKDKVADA 60 M + ++ +G P++GL+M M G + WQWLF++EG+P++ G L L ++ A+A Sbjct: 161 MTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANA 220 Query: 61 KWLSSEEKATIKAQLAQDDSKKATH-SFSAALASGRLWHLAFLYFCIALGNLGVTFWLPS 119 KWLS EK + LA ++S H SF A + R+W A +YFC+ GN + FW PS Sbjct: 221 KWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPS 280 Query: 120 LLRDAGVQSTSTIGWLAAIPFLFSAIVMVLVGRSADARAERRYHLAIPMVVGALGLVLAS 179 ++++ GV+ IG ++AIPF+ + M+ G +D ERR H AI ++ +GLV Sbjct: 281 IIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATG 340 Query: 180 W-MPNLPLLILMLTIATAGLTTALPMFWPLPSAILGGSAMAGGLAVLNSAGALAGFVGPS 238 + N L + LT+A G+ A P+FW +P A L G+A AGG+A++NS G LAGFV P Sbjct: 341 MTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPY 400 Query: 239 AIGYIRMNFAVPSLPLWLMAGIVLIGALTV 268 IG ++ + S L+ +A + L+ + V Sbjct: 401 MIGALKTSTGSLSSGLYFVAALELVASFLV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 903,140 Number of extensions: 37317 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 10 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)