BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1078.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 199 6e-53 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 195 1e-51 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 187 4e-49 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 181 2e-47 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 179 1e-46 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 199 bits (507), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 88/127 (69%), Positives = 110/127 (86%) Query: 18 YRKITFKILPFLLICYFFAYLDRVNVAFAKLQMAQHLQFSDAVYGLGAGIFFIGYFIFEV 77 Y K+T+++LP L +CY +YLDRVNV FAKLQM Q L+FSD VYGLGAGIFF+GYF+FEV Sbjct: 21 YSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEV 80 Query: 78 PSNLIMERVGAKRWISRIMVTWGLLSAGMAFVTTAEQFYVMRFLLGAAEAGFFPGVLLYL 137 PSN+I+ +VGA+ WI+RIM+TWG++S MA+VTT E FY+MRFLLG AEAGFFPGV+LYL Sbjct: 81 PSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYL 140 Query: 138 SYWFPPA 144 +YW+P A Sbjct: 141 TYWYPAA 147 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 195 bits (496), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 88/144 (61%), Positives = 116/144 (80%), Gaps = 3/144 (2%) Query: 2 STSTAALG---TVPETTGIYRKITFKILPFLLICYFFAYLDRVNVAFAKLQMAQHLQFSD 58 + ST +G G+Y+K+ ++I+PFL++CY AYLDRVNV FAKLQM+Q L FS+ Sbjct: 3 TASTTGVGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSE 62 Query: 59 AVYGLGAGIFFIGYFIFEVPSNLIMERVGAKRWISRIMVTWGLLSAGMAFVTTAEQFYVM 118 V+GLGAG+FFIGYF+FEVPSN++M +VGA+ WI+RIM+TWG+LSA FV A QFY++ Sbjct: 63 TVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYIL 122 Query: 119 RFLLGAAEAGFFPGVLLYLSYWFP 142 RFLLG AEAGF+PG++LYL+YWFP Sbjct: 123 RFLLGLAEAGFYPGIILYLTYWFP 146 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 187 bits (474), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 90/153 (58%), Positives = 117/153 (76%), Gaps = 9/153 (5%) Query: 4 STAALGTVPETTGIYRKITFKILPFLLICYFFAYLDRVNVAFAKLQMAQHLQFSDAVYGL 63 + A+ G+ + G+Y+K+T+KILP+LL+ Y AYLDRVNV FAKL M L S+A YGL Sbjct: 2 ANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGL 61 Query: 64 GAGIFFIGYFIFEVPSNLIMERVGAKRWISRIMVTWGLLSAGMAF---------VTTAEQ 114 GAGIFF+GYFIFEVPSNLI+ RVGAK WI+RIM+TWG++S+ MAF V+ Sbjct: 62 GAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETV 121 Query: 115 FYVMRFLLGAAEAGFFPGVLLYLSYWFPPALNR 147 FY +RFLLGA+EAGFFPGV+LY++YW+P A++ Sbjct: 122 FYTLRFLLGASEAGFFPGVILYMTYWYPAAMHS 154 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 181 bits (460), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 83/125 (66%), Positives = 106/125 (84%) Query: 18 YRKITFKILPFLLICYFFAYLDRVNVAFAKLQMAQHLQFSDAVYGLGAGIFFIGYFIFEV 77 YRKI ++++PFL+ + A++DRVNV FAKLQM Q LQFS+AVYGLGAGIFFIGYFIFEV Sbjct: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 78 PSNLIMERVGAKRWISRIMVTWGLLSAGMAFVTTAEQFYVMRFLLGAAEAGFFPGVLLYL 137 PSNL++E++GA++ ++RI + WGL S MA+VTT FY++RFLLG EAGFFPGV+LYL Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYL 144 Query: 138 SYWFP 142 +YWFP Sbjct: 145 TYWFP 149 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 179 bits (453), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 86/146 (58%), Positives = 105/146 (71%), Gaps = 5/146 (3%) Query: 2 STSTAALGTVPE-----TTGIYRKITFKILPFLLICYFFAYLDRVNVAFAKLQMAQHLQF 56 S+ A L T P IYRK+ +I+PFL ICY ++LDR+N+ FA+LQM Q L F Sbjct: 4 SSGGATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGF 63 Query: 57 SDAVYGLGAGIFFIGYFIFEVPSNLIMERVGAKRWISRIMVTWGLLSAGMAFVTTAEQFY 116 SDA+YGLGA IF++GY +FEVPSN+ + R GA+R SRIMV WGL S GM VT A FY Sbjct: 64 SDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFY 123 Query: 117 VMRFLLGAAEAGFFPGVLLYLSYWFP 142 +RFLLG EAGFFPG++LYLSYWFP Sbjct: 124 TLRFLLGVFEAGFFPGIVLYLSYWFP 149 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.145 0.458 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 397,560 Number of extensions: 12602 Number of successful extensions: 83 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 83 Number of HSP's successfully gapped: 12 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 126 (53.1 bits)