BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1076.p (486 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 457 e-129 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 276 3e-75 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 259 5e-70 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 240 2e-64 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 233 2e-62 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 457 bits (1175), Expect = e-129, Method: Compositional matrix adjust. Identities = 238/481 (49%), Positives = 328/481 (68%), Gaps = 2/481 (0%) Query: 6 SQKWSSKIYSNGWQTGTEKPHSVVEPATGELLGTVGVGSSADIARAAEIAAAAQRAWAET 65 S +W ++ W + +V+EPATG L VG+ S D++ + E+A AAQ AW Sbjct: 9 SAQWQQHLFRGQWVAAAQGA-TVLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAM 67 Query: 66 TFTVRAEILRKAASLLRQRAEEFTYWNIRECGSIRPKADFELDATYEQLQMAAGMLLQPD 125 RA+I R+AA L Q +E RE G + K + E+ +AAGM LQ Sbjct: 68 PPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQ 127 Query: 126 GLMYPSTMPGRLNFTRHVPIGAVGVISPWNFPLLLGMRSVAPALAMGNSVILKPDVQSAI 185 G + PST PGRL+ R P+G +GVISP+NFPL+L +R+VAPALA GN+V++KPD+++ + Sbjct: 128 GQLLPST-PGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPV 186 Query: 186 TGGALIAEIFEEAGLPPGVLHVIPGAAATGDALVRHPKVGMISFTGSTAVGRQIGAICGQ 245 +GG ++A IFE+AGLP G+LHV+PG A TG+ALV P V MI+FTGS AVGR+IG + G+ Sbjct: 187 SGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRIGELAGR 246 Query: 246 TLKKVSLELGGNNVQIVLEDADLDAAASSGAWGSFLHQGQICMQTGRHLVHKSVAKQYAE 305 LKK+SLELGG N ++LEDADLD AAS AWG++LHQGQICM R LVH S+ + + Sbjct: 247 HLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQ 306 Query: 306 KLAERAQRLQVGNPFKDQVHLGPLINERQRDRIAGIVKSSVDTGAKVLAGGTYKDLFFQA 365 +LA++A+ L VG+ QV LGP+I+ERQ R+ +V+ SV GA+++AGG Y+ LF+Q Sbjct: 307 RLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLFYQP 366 Query: 366 TVLGDVTSDMPVFTEEIFGPVAPITVFEDEDHAVELANSSEYGLAAAIHTKSVARGIALA 425 TVLG V M VF EEIFGPVA + F++++ A+ LAN GLAA + + SV R +A+A Sbjct: 367 TVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMAVA 426 Query: 426 SRVRAGMVHINDQTVNNEFHVPFGGMGASGNGGRFGGPANFHEFTQTQWVSAVERGIQYP 485 +R+R GMVH+NDQTVN+E PFGG G +GNG GGPA++ E+TQ +W + ++ + YP Sbjct: 427 ARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQAMPYP 486 Query: 486 F 486 F Sbjct: 487 F 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 276 bits (705), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 169/421 (40%), Positives = 249/421 (59%), Gaps = 6/421 (1%) Query: 70 RAEILRKAASLLRQRAEEFTYWNIRECGSIRPKADFELDATYEQLQMAAGMLLQPDGLMY 129 R +L AA L+ RA++F E G+ A F + + L+ AA M Q G + Sbjct: 65 RRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEVI 124 Query: 130 PSTMPGRLNFTRHVPIGAVGVISPWNFPLLLGMRSVAPALAMGNSVILKPDVQSAITGGA 189 PS +PG L P G V I+PWN P++LG+R++A LA GN+V+ K T G Sbjct: 125 PSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHGL 184 Query: 190 LIAEIFEEAGLPPGVLHVIPGA----AATGDALVRHPKVGMISFTGSTAVGRQIGAICGQ 245 +IA + +EAGLPPGV++ + A AA +A++ P V ++FTGST VGR I A C + Sbjct: 185 IIAAL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQCAR 243 Query: 246 TLKKVSLELGGNNVQIVLEDADLDAAASSGAWGSFLHQGQICMQTGRHLVHKSVAKQYAE 305 LK LELGG IVL DADLDAA + A+G+F + GQICM T R +V SVA ++ Sbjct: 244 HLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFVR 303 Query: 306 KLAERAQRLQVGNPFKDQVHLGPLINERQRDRIAGIVKSSVDTGAKVLAGGTYKDLFFQA 365 +LA +A L +G+P + V LG +++ +R ++ ++ GA ++ GG D A Sbjct: 304 RLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTLMPA 363 Query: 366 TVLGDVTSDMPVFTEEIFGPVAPITVFEDEDHAVELANSSEYGLAAAIHTKSVARGIALA 425 T+L V+++M ++ EE FGPV PI ++ A+ AN +EYGL++A+ ++ VAR +AL+ Sbjct: 364 TLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARALALS 423 Query: 426 SRVRAGMVHINDQTVNNEFHVPFGGMGASGNGGRFGGPANFHEFTQTQWVSAVERGIQYP 485 R+ +G+ HIN TV++E +PFGG+ +SG G FGG A EFT+ +WV+ YP Sbjct: 424 RRIESGICHINGPTVHDEAQMPFGGVKSSGF-GHFGGKAGIAEFTELRWVTVQTTPRHYP 482 Query: 486 F 486 F Sbjct: 483 F 483 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 259 bits (661), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 162/474 (34%), Positives = 252/474 (53%), Gaps = 9/474 (1%) Query: 12 KIYSNGWQTGTEKPHS--VVEPATGELLGTVGVGSSADIARAAEIAAAAQRAWAETTFTV 69 +++ NG T + + V+ PAT E++G + AD+ RA E A+ W + Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 70 RAEILRKAASLLRQRAEEFTYWNIRECGSIRPKADFELDATYEQLQMAAGMLLQPDGLMY 129 R++I+R+AA LLR+RAEE E G +A E + + A + G + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 130 PSTMPGRLNFTRHVPIGAVGVISPWNFPLLLGMRSVAPALAMGNSVILKPDVQSAITGGA 189 P+ +PG P+G V +PWNFPL +R ++ ALA G S+I+K ++ + Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 190 LIAEIFEEAGLPPGVLHVIPGAAA-TGDALVRHPKVGMISFTGSTAVGRQIGAICGQTLK 248 LI + +AG+P GV++++ G A + L+ HP + ISFTGST VG+Q+ A+ GQ +K Sbjct: 186 LI-RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 249 KVSLELGGNNVQIVLEDADLDAAASSGAWGSFLHQGQICMQTGRHLVHKSVAKQYAEKLA 308 + ++ELGG+ +V +DAD+D AA A F + GQ+C+ R LV K V + +K Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 309 ERAQRLQVGNPFKDQVHLGPLINERQRDRIAGIVKSSVDTGAKVLAGGTY---KDLFFQA 365 A++L+VGN + + +GP+ N+R+ + ++ +V GA++ GG K FFQ Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQP 364 Query: 366 TVLGDVTSDMPVFTEEIFGPVAPITVFEDEDHAVELANSSEYGLAAAIHTKSVARGIALA 425 TVL ++ EE FGP+A I FE D + AN YGLAA KS ALA Sbjct: 365 TVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALA 424 Query: 426 SRVRAGMVHINDQTVNNEFHVPFGGMGASGNGGRFGGPANFHEFTQTQWVSAVE 479 +RV +GM+ IN + +PFGG+ SG G GG + T+ V+ ++ Sbjct: 425 ARVESGMLTINHLGLALP-ELPFGGIKDSGYGSE-GGTEAIEAYINTKLVTQLD 476 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 240 bits (612), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 160/435 (36%), Positives = 226/435 (51%), Gaps = 6/435 (1%) Query: 27 SVVEPATGELLGTVGVGSSADIARAAEIAAAAQRAWAETTFTVRAEILRKAASLLRQRAE 86 +V PA GELLG V + +A+ RA E A AA AW + T R+ ILR+ L+ + Sbjct: 30 AVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELMLANTD 89 Query: 87 EFTYWNIRECGSIRPKADFELDATYEQLQMAAGMLLQPDGLMYPSTMPGRLNFTRHVPIG 146 + E G +A E+ ++ A + G PS P IG Sbjct: 90 DLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIG 149 Query: 147 AVGVISPWNFPLLLGMRSVAPALAMGNSVILKPDVQSAITGGALIAEIFEEAGLPPGVLH 206 I+PWNFP + R PALA G ++LKP + + AL A + E AG+P GV Sbjct: 150 VCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALAL-AVLAERAGIPAGVFS 208 Query: 207 VIPGA-AATGDALVRHPKVGMISFTGSTAVGRQIGAICGQTLKKVSLELGGNNVQIVLED 265 V+ G G + +P V ISFTGST VG+ + ++KK+SLELGGN IV +D Sbjct: 209 VVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDD 268 Query: 266 ADLDAAASSGAWGSFLHQGQICMQTGRHLVHKSVAKQYAEKLAERAQRLQVGNPFKDQVH 325 ADLDAA + + GQ C+ R V V +A KL E ++ +VG ++ V Sbjct: 269 ADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVT 328 Query: 326 LGPLINERQRDRIAGIVKSSVDTGAKVLAGGTYKDL---FFQATVLGDVTSDMPVFTEEI 382 GPLINE+ ++ V +V GA+VL GG L FF+ TVL DVT M V EE Sbjct: 329 QGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAMQVAREET 388 Query: 383 FGPVAPITVFEDEDHAVELANSSEYGLAAAIHTKSVARGIALASRVRAGMVHINDQTVNN 442 FGP+AP+ F+ ++ + LAN +E+GLA+ +++ + R +A + +GMV IN ++N Sbjct: 389 FGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISN 448 Query: 443 EFHVPFGGMGASGNG 457 E PFGG+ SG G Sbjct: 449 EV-APFGGVKQSGLG 462 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 233 bits (595), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 158/430 (36%), Positives = 243/430 (56%), Gaps = 19/430 (4%) Query: 61 AWAETTFTVRAEILRKAASLLRQRAEEFTYWNIRECGS--IRPKADFELDATYEQLQMAA 118 AW++ + R ++L K A+L+ + + + R G + P+ ++ L+ AA Sbjct: 59 AWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRM-MDVPNAISHLRAAA 117 Query: 119 GMLLQPDGLMYPST-MPGR--LNFTRHVPIGAVGVISPWNFPLLLGMRSVAPALAMGNSV 175 G Q +G P+ G+ L++T PIG VG I PWN PL++ VA LA G +V Sbjct: 118 GWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTV 177 Query: 176 ILKP---DVQSAITGGALIAEIFEEAGLPPGVLHVIPG-AAATGDALVRHPKVGMISFTG 231 ++KP QSA+ +A + +EAG P GV++V+ G + G AL HP V ISFTG Sbjct: 178 VIKPAEETPQSALH----LARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTG 233 Query: 232 STAVGRQIGAICGQTLKKVSLELGGNNVQIVLEDADLDAAASSGAWGSFLHQGQICMQTG 291 S GR I I G+ K+V+LELGG + QIV +DA + A G F++QGQ+C Sbjct: 234 SPEAGRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGS 293 Query: 292 RHLVHKSVAKQYAEKLAERAQRLQVGNPFKDQVHLGPLINERQRDRIAGIVKSSVDTGAK 351 R LV +S+A+++A+ LAE A + VG+P + V +GP+ + Q +R+ ++ +D GA Sbjct: 294 RILVQRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGAT 353 Query: 352 VLAGGTY---KDLFFQATVLGDVTSDMPVFTEEIFGPVAPITVFEDEDHAVELANSSEYG 408 +LAGG K F Q T+ D + M + EEIFGPV I F+ E+ A+ LAN S+YG Sbjct: 354 LLAGGVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYG 413 Query: 409 LAAAIHTKSVARGIALASRVRAGMVHINDQTVNNEFHVPFGGMGASGNGGRFGGPANFHE 468 LAA + T ++AR +A+ V+ G V +N + + ++P+GG+ SG GR GG A Sbjct: 414 LAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDA-NLPWGGIKDSGM-GREGGLAGALA 471 Query: 469 FTQTQWVSAV 478 +T+ + V+ + Sbjct: 472 YTEEKTVTVL 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,496,068 Number of extensions: 63186 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 20 Length of query: 486 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 397 Effective length of database: 1,198,455 Effective search space: 475786635 Effective search space used: 475786635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)