BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1075.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 83 3e-17 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 81 1e-16 gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046... 63 4e-11 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 62 6e-11 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 62 8e-11 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 74/266 (27%), Positives = 118/266 (44%), Gaps = 12/266 (4%) Query: 102 RMAMAGIDMAAWDALAKLHEVPLVTLLGGTPK-KIP-AYDSHSMDGIEVGSKRAAEAAER 159 R A I+ A DALA+ VPL TL GG + +P A+ S D + A+R Sbjct: 106 RFAKCAIETALSDALARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQR 165 Query: 160 GYQAIKTKIGYQTLEEDLKIVRTLRSIIGDEVQLMVDYNQGLTIPEAIRRGKALEQENIA 219 + K KIG +T+++D V ++ +G+ + VD NQ T EA+ + L + Sbjct: 166 RHNLFKLKIGARTVQQDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVD 225 Query: 220 WIEEPTLQQDYEGHAAIRQALNIPVQMGENWFGPDEMAKAINAGASDFAMPDLMKIGGVT 279 +E+P +++G +RQ I + E+ GP + + AGA+D + + GG+ Sbjct: 226 LVEQPIAASNHDGLRRLRQKNRIAIMADESLHGPQDAYQLARAGAADVFAVKINQSGGLA 285 Query: 280 GWLRAAALAEQK------GIPLSSHIFQEFSAHLLAVSPTCHWIERMD----LAGAITEP 329 G + AA+A G L I SA L A T W + L I Sbjct: 286 GAQQVAAIASAAQVELYGGTMLEGAIGTMASAQLFATFGTLAWGSELFGPLLLTEEILAT 345 Query: 330 TLQFKDGQAIVPDMPGAGLSWRESEI 355 L+++D +P PG G++ E + Sbjct: 346 PLRYRDFALELPTGPGLGITLDEDRV 371 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 81.3 bits (199), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 79/313 (25%), Positives = 128/313 (40%), Gaps = 24/313 (7%) Query: 67 VKDLEQILVGQPLAPYELDRLLCARFRLIGTTGLVRMAMAGIDMAAWDALAKLHEVPLVT 126 V++L L+G+ E + R + A+AGID A WD K VP+ Sbjct: 45 VEELSDYLIGKDPRNIEDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQ 104 Query: 127 LLGGTPKK-IPAYDSHSMDGIEVGSKRAAEAAERGYQAIKTK--------IGYQTLEEDL 177 LLGG + I Y D + A A RG+ A+K + +E L Sbjct: 105 LLGGAVRNSIRVYSWIGGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTL 164 Query: 178 KIVRTLRSIIGDEVQLMVDYNQGLTIPEAIRRGKALEQENIAWIEEPTLQQDYEGHAAIR 237 V+ +R +G V + VD++ + P A K LE + +IEEP L ++ E I Sbjct: 165 ANVQAVREAVGPNVGIGVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIA 224 Query: 238 QALNIPVQMGENWFGPDEMAKAINAGASDFAMPDLMKIGGVTGWLRAAALAEQKGIPLSS 297 + P+ +GE + + + ++ G D PD+ GG+T + A +AE + L+ Sbjct: 225 HLTSTPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALAL 284 Query: 298 HI--------------FQEFSAHLLAVSPTCHWIERMDLAGAITEPT-LQFKDGQAIVPD 342 H ++A + S H+ E DL I + ++DG +P Sbjct: 285 HCPLGPIALASCLQVDAGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQ 344 Query: 343 MPGAGLSWRESEI 355 PG G+ E + Sbjct: 345 GPGLGIEINEEYV 357 >gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046 [Herbaspirillum seropedicae SmR1] Length = 425 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 49/165 (29%), Positives = 75/165 (45%), Gaps = 6/165 (3%) Query: 155 EAAERGYQAIKTKIGYQTLEEDLKIVRTLRSIIGDEVQLMVDYNQGLTIPEAIRRGKALE 214 EA ++G+ +K K+G + L +D + V R ++G E +LM+D NQ + +AI L Sbjct: 207 EAIDQGFNHVKLKVG-RDLADDKRRVTIAREVLGPERKLMIDANQVWEVHQAIDWVNQLA 265 Query: 215 QENIAWIEEPTLQQDYEGHAAIRQALN-IPVQMGENWFGPDEMAKAINAGASDFAMPDLM 273 +IEEPT D EGH IR+ + + V GE + I A D D Sbjct: 266 FAQPWFIEEPTSPDDVEGHRKIREGIGAVKVATGEMCQNRVLFKQFIMRDAIDVVQIDSC 325 Query: 274 KIGGVTGWLRAAALAEQKGIPLSSHI----FQEFSAHLLAVSPTC 314 ++GGV L +A + G + H E+ HL + C Sbjct: 326 RLGGVNEILAVMLMAAKYGKVVCPHAGGVGLCEYVQHLSMIDYLC 370 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 67/269 (24%), Positives = 108/269 (40%), Gaps = 14/269 (5%) Query: 102 RMAMAGIDMAAWDALAKLHEVPLVTLLGGTPKK-IP-AYDSHSMDGIEVGSKRAAEAAER 159 R A I+ A +DAL + +P+ LLGG ++ +P A+ S D + + A+R Sbjct: 112 RFAKCAIETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQR 171 Query: 160 GYQAIKTKIGYQTLEEDLKIVRTLRSIIGDEVQLMVDYNQGLTIPEAIRRGKALEQENIA 219 + K KIG + ++ D+ V ++ +GD + VD N + EA R L Sbjct: 172 RHNIFKLKIGRREVDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCE 231 Query: 220 WIEEPTLQQDYEGHAAIRQALNIPVQMGENWFGPDEMAKAINAGASDFAMPDLMKIGGVT 279 +E+P R A I + E+ GP G +D + + GG+ Sbjct: 232 LVEQPVASPQALARLKGRHA--IAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLR 289 Query: 280 GWLRAAALAEQKGIPLSSHIFQE------FSAHLLAVSPTCHWIERMD----LAGAITEP 329 + AA+ + GI L E SAH A W + L I Sbjct: 290 AAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAE 349 Query: 330 TLQFKDGQAIVPDMPGAGLSWRESEIAKY 358 LQ++D Q +P PG G++ E + + Sbjct: 350 PLQYQDFQLAIPHRPGLGITLDEERLQYF 378 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 62.0 bits (149), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 69/291 (23%), Positives = 120/291 (41%), Gaps = 40/291 (13%) Query: 101 VRMAMAGIDMAAWDALAKLHEVPLVTLLGGTPKK-IP-------AYDSH----------- 141 V A + ++A DA A+ VPLV LLGG +K IP Y H Sbjct: 107 VSNAYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDAWG 166 Query: 142 ---SMDGIEVGSKRAAEAAERGYQAIKTKIGYQTLEEDLKIVRTLRSIIGDEVQLMVDYN 198 S + I +++ E E G+Q+IK K G E ++ ++ LR D L +D N Sbjct: 167 EALSEEQIVAQARKMIE--ENGFQSIKLKAGALAPEHEVACIKALRKAFPD-APLRIDPN 223 Query: 199 QGLTIPEAIRRGKALEQENIAWIEEPTLQQDYEGHAAIRQALNIPVQMGENWFGPDEMAK 258 ++ AIR + L +++ + E+PT EG A + + +P+ DE+ + Sbjct: 224 GNWSLQTAIRMAELL-GDDLQYYEDPT--PGLEGMAELHRRTGLPLATNMVVTDFDELRR 280 Query: 259 AINAGASDFAMPDLMKIGGVTGWLRAAALAEQKGIPLSSHIFQEFSAHLLAVSPTCHWIE 318 ++ + + D GG+ + A + + G+ +S H L+A+S +E Sbjct: 281 SVALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVE 340 Query: 319 RMDLAGAITEP------------TLQFKDGQAIVPDMPGAGLSWRESEIAK 357 + A P L ++G + D PG G+ + ++ K Sbjct: 341 NLSYACDTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEVDQDQLRK 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,175,948 Number of extensions: 50669 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 5 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)