BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1073.p (526 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 395 e-111 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 115 7e-27 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 114 3e-26 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 67 5e-12 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 395 bits (1014), Expect = e-111, Method: Compositional matrix adjust. Identities = 227/530 (42%), Positives = 304/530 (57%), Gaps = 15/530 (2%) Query: 3 TVHQASYALLREQGMTTFFGNPGSNELPFLKDFPKDFRYILGLHEGAVVGMADGYAQATS 62 TV A L+R GMT+ F NPGS ELP +DFP+DFRY+LGL E VVGMADG+AQA+ Sbjct: 10 TVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPEDFRYVLGLQEAVVVGMADGFAQASG 69 Query: 63 KPAFVNLHSAAGTGNAMGALANAWNSHTPLVVTAGQQVRPMIGVEALLANVDSAMLPRPL 122 A VNLHSAAG GNAMG + A+ + TPL++TAGQQ R ++ + L + L +P Sbjct: 70 NAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPY 129 Query: 123 VKWSCEPASAQEVPLALSRAIHTARSGAPGPVYVSVPYDDWAQPVGPGIEHLASRSVSSA 182 +KWS EPA A +VPLAL+RA H A GPV+VS+P DDW QP E + V+S Sbjct: 130 LKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPA----ELVPPTQVTSR 185 Query: 183 TIPAPLQLESVLSALAKAQNPVLIVGPDVDAAHANRDAVTLAEKLGMPVWMAPSAPRCSF 242 P P + + +AL ++Q P ++VG ++D A A A+ LAE L V++AP + RCSF Sbjct: 186 CRPEPAAIARLAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSF 245 Query: 243 PTDHQCFQGLLPAGIATIGRLMEGHDLVLVVGAPVFRYHQYDPGEFLPNGTKLIQITCDI 302 P DH F G LPA I + ++GHDL+ +GAP F YH G +P G L Q+ D Sbjct: 246 PEDHPLFAGFLPAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLIDDP 305 Query: 303 NEATRAPFGDAIIADIGPTLKALAEGAAKSGRPLPAVKKRPEPAP-----EQPGPLAPER 357 A+ P G AI+ +I L L A+ R P + +P P E PL+ Sbjct: 306 VTASWTPTGQAIVGNIQLGLDDL---LAQIRRQPPTQRAQPPARPARARVEASAPLSMAY 362 Query: 358 VFDILDAVAPRDAVYVNESTSTTNIMWQRLRLKEQGSYFFAAAGGLGFALPAAVGIQLAH 417 L V + V E+ + +M L GS++ A+GGLG+ +PAAVG+ LA Sbjct: 363 ALQTLADVREAGDIVVEEAPGSRPVMQAHLPFTRPGSFYTMASGGLGYGMPAAVGVALAR 422 Query: 418 KDRRVIGVIGDGSANYGIQALWTAAQYNIPTIFVIMKNGTYGALRWFAGVLKV---DDVP 474 RVIG+IGDGSA Y QA+++A Q +P FVI+ NG Y AL+ FA V D V Sbjct: 423 PHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDPVH 482 Query: 475 GLDVPGLDFCALAKGYGVKSYRANSSESFTAALKSALDTNEPVLIEVETR 524 G ++PGLDF ALA G R + + + AL+ AL +NE L+E+ R Sbjct: 483 GTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLVEIVVR 532 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 115 bits (289), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 142/547 (25%), Positives = 219/547 (40%), Gaps = 69/547 (12%) Query: 6 QASYALLREQGMTTFFGNPGSNELPF---LKDFPKDFRYILGLHEGAVVGMADGYAQATS 62 A +A L +QG+ T F NPG+ EL L+ FPK R ++ L E G ADGY + Sbjct: 40 HALWAGLLDQGVDTCFANPGTTELDLVRGLESFPK-LRCVVALQENVCTGAADGYGRMLG 98 Query: 63 KPAFVNLHSAAGTGNAMGALANAWNSHTPLVVTAGQQVRPMIGVEALLANVDSAMLPRPL 122 +PA LH G N + L NA + TP+V G + +A L++ D L P+ Sbjct: 99 RPASTLLHLGPGFANGIANLHNARRARTPIVNIIGDHTSWHLPYDAPLSS-DIESLAHPV 157 Query: 123 VKWSCEPASAQEVPLALSRAIHTARSGAPGPVYVSVPYD-DWAQPVGPGIEHLASRSVSS 181 W ++ A + ++ + + + P D +A +A S ++ Sbjct: 158 SGWVHRITGTADIAAAAAESVRQSTAAGGQGSTLIFPADFQYAD--------MAEASPAA 209 Query: 182 ATIPAPLQLESVLS--ALAKAQNPVLIV-------GPDVDAAHANRDAVTLAEKLGMPVW 232 AT Q + + ALA+ Q +V A A R +A +G V+ Sbjct: 210 ATEKDTGQADDYRADQALARLQGAQRVVFLLGGGGEESGLGARAQRAVARIAAHIGCSVY 269 Query: 233 MAPSAPRCSFPTDHQCFQGL-----LPAGIATIGRLMEGHDLVLVVGA--PV--FRYHQY 283 +FP + QGL LP ++++ DLV++VGA P+ F Y + Sbjct: 270 AE------TFPARAERGQGLPSFDRLPYFPEPARKVLDDADLVVLVGALPPITYFGYVGH 323 Query: 284 DPGEFLPNGTKLIQITCDINEATRAPFGDAIIADIGPTLKALAEGAAKSGRPLPAVKKRP 343 P I + DA+ GA P A+ P Sbjct: 324 PSALVAPERLLTISLPGRPAAGRVEQLADAL-------------GAPAYVTPQHALPPLP 370 Query: 344 EPAPEQPGPLAPERVFDILDAVAPRDAVYVNESTSTTNIMWQRLRLKEQGSYFFAAAGGL 403 E G L P+++ +L P A+ E + + + G + Sbjct: 371 E------GELTPQKIAAVLARELPERAIVSVEGGTCGYPFYAVSDAARPHTVLTNTGGAI 424 Query: 404 GFALPAAVGIQLAHKDRRVIGVIGDGSANYGIQALWTAAQYNIPTIFVIMKNGTYGALRW 463 G LP A+G +A DR V+G++ DGS Y IQ LW+ A N + +I N Y LR Sbjct: 425 GQGLPVALGAAVACPDRTVVGLLSDGSTQYTIQTLWSLAHENARVVVLIAANHQYAILR- 483 Query: 464 FAGVLKVDDVP---------GLDVPGLDFCALAKGYGVKSYRANSSESFTAALKSALDTN 514 L+ D P LD P +D+ LA+ YGV + RA +++ L++A Sbjct: 484 --NELRRDGAPLGERAAQMTALDHPRIDWVGLAQSYGVPASRAETAQQLQQQLQAAFAGT 541 Query: 515 EPVLIEV 521 P LIE+ Sbjct: 542 GPALIEM 548 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 114 bits (284), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 136/538 (25%), Positives = 220/538 (40%), Gaps = 58/538 (10%) Query: 12 LREQGMTTFFGNPGSNELPFLKD--FPKD-FRYILGLHEGAVVGMADGYAQATSKPAFVN 68 L E+G+ FG PG L ++ D F +D F++IL HE A V AD Y++++ K Sbjct: 16 LAEEGVEHVFGYPGGAVL-YIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAI 74 Query: 69 LHSAAGTGNAMGALANAWNSHTPLVVTAGQQVRPMIGVEALLANVDSAMLPRPLVKWSCE 128 + S G NA+ LA A+ P+VV +GQ IG +A D+ + RP VK + Sbjct: 75 VTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDA-FQECDTVGITRPCVKHNFL 133 Query: 129 PASAQEVPLALSRAIHTARSGAPGPVYVSVPYDDWAQPVGPGI-EHLASRSVSSATIPAP 187 +++ + +A + A +G PGPV V +P D + + RS Sbjct: 134 VKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYKPVDKGHS 193 Query: 188 LQLESVLSALAKAQNPVLIVGPDVDAAHANRDAVTLAEKLGMPV---WMAPSAPRCS--- 241 Q+ L L A+ P++ G + A+A + L ++LG P M A R S Sbjct: 194 GQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYRSSSDK 253 Query: 242 ---FPTDHQCFQGLLPAGIATIGRLMEGHDLVLVVGAPVFRYHQYDPGEFLPNGTKLIQI 298 P H G A +A M+ D+++ +GA +P F + K+I I Sbjct: 254 FVGMPGMH----GTYEANMA-----MQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHI 304 Query: 299 TCDINE-ATRAPFGDAIIADIGPTLKAL-----AEGAAKSGRPLPAVKKRPEPAPEQPGP 352 D + + R I+ ++ L+ L A ++G L A K+ ++ Sbjct: 305 DIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCL 364 Query: 353 --------LAPERVFDILDAVAPRDAVYVNESTSTTNIMWQRLRLKEQGSYFFAAAGGL- 403 + P+ V + V DA S + MW + +GGL Sbjct: 365 KFATSEEFIKPQSVVQKVWEVTKGDAFIT--SDVGQHQMWAAQYYGFDKPRRWINSGGLG 422 Query: 404 --GFALPAAVGIQLAHKDRRVIGVIGDGSANYGIQALWTAAQYNIPTIFVIMKNGTYGAL 461 G LP A+G+Q+A+ D V + G+ S IQ L T QY++ +++ N G + Sbjct: 423 TMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMV 482 Query: 462 RWFAGV--------LKVDDVPGLDVPGLDFCALAKGYGVKSYRANSSESFTAALKSAL 511 R + + +D +P DF LA+ YG + AL+ A Sbjct: 483 RQWQQIDYGSRYSESYMDSLP-------DFNKLAESYGHVGMKIEKPGDVDGALREAF 533 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 66.6 bits (161), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 114/533 (21%), Positives = 200/533 (37%), Gaps = 40/533 (7%) Query: 12 LREQGMTTFFGNPGSNELPFLKDFPKDFR--YILGLHEGAVVGMADGYAQATSKPAFVNL 69 L E G+ FG G P +D R +I HE A G A+ T + A Sbjct: 13 LYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKLTGRLAVCCG 72 Query: 70 HSAAGTGNAMGALANAWNSHTPLVVTAGQQVRPMIGVEALLANVDSAMLPRPLVKWSCEP 129 + G + + L A H P++ +G G + L N +L R + +S Sbjct: 73 TTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQEN-SPELLFRDVAAYSQII 131 Query: 130 ASAQEVPLALSRAIHTARSGAPGPVYVSVPYDDWAQPVGPGIEHLASRSVSSATIPAPLQ 189 S + P +AI A + G ++++P D + L + + P Sbjct: 132 TSPDQAPAVFHQAIAQAYA-QRGVAHLNIPADVIGAKTSGSVPSLHTLRERTPLSPPAAD 190 Query: 190 LESVLSALAKAQNPVLIVGPDVDAAHANRDAVTLAEKLGMPVWMAPSAPRCSFPTDHQCF 249 +++ + A + + G A + + LA KL PV M + DH + Sbjct: 191 IQAAADLINAAGSVAIFAGNGCRGA--IDEVLALASKLQAPV-MHTFRGKDLASYDHPHW 247 Query: 250 QGLLPAGIATIG-----RLMEGHDLVLVVGAPVFRYHQYDPGEFLPNGTKLIQI-TCDIN 303 G G+ IG + +++L++G+ Y EFLP +K+IQI Sbjct: 248 IG----GVGLIGGAPGTDALHAAEVMLMLGS------DYPYSEFLPTKSKVIQIDERGFV 297 Query: 304 EATRAPFGDAIIADIGPTLKALAE--GAAKSGRPLPAVKKRPE----------PAPEQPG 351 RAP I + P ++ L E G L E Sbjct: 298 LGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHK 357 Query: 352 PLAPERVFDILDAVAPRDAVYVNESTSTTNIMWQRLRLKEQGSYFFAAA---GGLGFALP 408 + P+ V + +A DA +V ++ T +W ++++G A+ +G +L Sbjct: 358 KIKPQSVARAISDLADDDAAFVVDTGVVT--LWCGNWIRQRGGQRILASFNNAAVGTSLG 415 Query: 409 AAVGIQLAHKDRRVIGVIGDGSANYGIQALWTAAQYNIPTIFVIMKNGTYGALRWFAGVL 468 A GIQ ++R+V+ +GDG + T+ ++ +P V+ N +G + Sbjct: 416 QANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEA 475 Query: 469 KVDDVPGLDVPGLDFCALAKGYGVKSYRANSSESFTAALKSALDTNEPVLIEV 521 + G + P DF A A+ G + + + E L+ L P +++V Sbjct: 476 GMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDV 528 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,829,495 Number of extensions: 86538 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 5 Length of query: 526 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 436 Effective length of database: 1,193,720 Effective search space: 520461920 Effective search space used: 520461920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)