BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.1071.p
         (281 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2...   554   e-159
gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578...   112   4e-26

>gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 281

 Score =  554 bits (1427), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 268/281 (95%), Positives = 278/281 (98%)

Query: 1   MKLLRYGPVGQEKPGILDQSGKIRDLSAYIKDVNGAVLDDASLDKIRKLDLESLPAVEGS 60
           MKLLRYGPVGQEKPGILDQ+GKIRDLSA+IKDVNGAVLDDASL+KIRKLDL +LP VEG+
Sbjct: 1   MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60

Query: 61  PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 120
           PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPND+VKIPRGSKK
Sbjct: 61  PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120

Query: 121 TDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180
           TDWEVELGV+IGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP
Sbjct: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180

Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRFMSLQPGDVIS 240
           IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIF VAHIVSYLSRFMSLQPGDVIS
Sbjct: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240

Query: 241 TGTPPGVGMGVKPEAVYLRAGQTMRLGIDGLGEQQQRTIDA 281
           TGTPPGVGMGVKPEAVYLRAGQT+RLGIDGLGEQ+Q+TIDA
Sbjct: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA 281


>gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578
           [Herbaspirillum seropedicae SmR1]
          Length = 227

 Score =  112 bits (279), Expect = 4e-26,   Method: Compositional matrix adjust.
 Identities = 77/221 (34%), Positives = 109/221 (49%), Gaps = 13/221 (5%)

Query: 55  PAVEGSPRIGACVGNIGKFICIGLNYADHAAE-SNLPIPAEPVVFNKWTSAVVGPNDDVK 113
           PA      +G     + +  C+G NYA HA E  + P    P  F K   AVV  +  V 
Sbjct: 8   PAQATVAVVGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVP 67

Query: 114 IPRGSKKTDWEVELGVVIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIER---GGTWD 170
            P  +     E+EL V + KGG  I  + A+  V GY V  D++ R+ Q +    G  WD
Sbjct: 68  YPVATSDYHHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWD 127

Query: 171 KGKGCDTFGPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFGVAHIVSYLSRF 230
             KG D   P  P +V   +   P +  +WL+V+G+  Q G+ + +I+ VA  +SYLS  
Sbjct: 128 MAKGFDQSAPCAP-IVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGL 186

Query: 231 MSLQPGDVISTGTPPGVGMGVKPEAVYLRAGQTMRLGIDGL 271
           + L PGD+I TGTP GVG  VK        G  ++  +DGL
Sbjct: 187 VELFPGDLIFTGTPEGVGAVVK--------GDKLQGHVDGL 219


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,036,131
Number of extensions: 48571
Number of successful extensions: 120
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 119
Number of HSP's successfully gapped: 2
Length of query: 281
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 197
Effective length of database: 1,222,130
Effective search space: 240759610
Effective search space used: 240759610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 130 (54.7 bits)