BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1068.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310593|ref|YP_003774685.1| phosphate regulon two-component... 837 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 113 3e-26 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 111 9e-26 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 106 4e-24 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 104 2e-23 >gi|300310593|ref|YP_003774685.1| phosphate regulon two-component sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 837 bits (2162), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/433 (93%), Positives = 420/433 (96%) Query: 1 MSPQLLFWIPAVLRLVLCLGGAGVVGFFFGPIVGLVTAVLGVGAMMIAHLHYLLRLSTWL 60 MSPQLLFWIPAVLRLVLCL GAGVVGFFFGPI GLVTAVLGVGAMMI HLHYLLRLSTWL Sbjct: 1 MSPQLLFWIPAVLRLVLCLAGAGVVGFFFGPIAGLVTAVLGVGAMMITHLHYLLRLSTWL 60 Query: 61 DDAGQSRLPDGWGAWTDVYARLYRMHRDDEKNQAELTEWLARFRQAMSLLPDGVVIMDDV 120 DD+GQSRLPDGWGAWTDVYARLYRM RDDEKNQAEL EWLARFRQAMSLLPDGVVIMDDV Sbjct: 61 DDSGQSRLPDGWGAWTDVYARLYRMRRDDEKNQAELAEWLARFRQAMSLLPDGVVIMDDV 120 Query: 121 LFLEWCNPSAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTLALHERKLIIQ 180 LFLEWCNP+AQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLT+ LHERKLIIQ Sbjct: 121 LFLEWCNPAAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTMTLHERKLIIQ 180 Query: 181 IIPFENRRQILVTHDVTESQRIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLDHA 240 IIPFENRRQILVTHDVTES+RIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNL+ Sbjct: 181 IIPFENRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEG 240 Query: 241 TRTAHLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRSEVLRVRPLLERIAEEGRALSAG 300 TR AHLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLR+EVLRVRPLLERIAEEGRALSAG Sbjct: 241 TRMAHLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAG 300 Query: 301 KHDIRLIVDGPDLKGSADELRSAFTNLVTNAVRYTPEGGTITLRWESDEEGAHFSVQDTG 360 KH+I L +DGPDLKG+A+ELRSAFTNLVTNAVRYTPEGG I+LRW+SDE+GAHF+VQDTG Sbjct: 301 KHEIVLTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTG 360 Query: 361 IGISPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLDIASVPDHGSTFT 420 IGISPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATL+IASVPDHGSTFT Sbjct: 361 IGISPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFT 420 Query: 421 VHFPATQTIADYA 433 VHFP+TQTI DYA Sbjct: 421 VHFPSTQTIQDYA 433 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 85/246 (34%), Positives = 131/246 (53%), Gaps = 13/246 (5%) Query: 194 HDVTES--QRIDMMRRDF------VANASHELRTPLTVINGFLEIALAQP-NLDHATRTA 244 H++ ES + +D + R F A+ +H++RTPL + ++AL +P +D A Sbjct: 225 HEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQ--A 282 Query: 245 HLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRSEVLRVRPLLERIAEEGRALSAGKHDI 304 L E+ +R+ +++ ML L R E+ L+ + L V L RIA+ + A + + Sbjct: 283 LLSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGI-ADEAQV 341 Query: 305 RLIVDGPD-LKGSADELRSAFTNLVTNAVRYTPEGGTITLRWESDEEGAHFSVQDTGIGI 363 RL+V G L+ A LR A NLV NA+RYTP G I L+ ++ +G SV + G GI Sbjct: 342 RLVVQGAGRLQADATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGI 401 Query: 364 SPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLDIASVPDHGSTFTVHF 423 E + RL +RFYR D SRS +GLGLAIVR +++ H + S P + F++ F Sbjct: 402 PAEQLERLFDRFYRADPSRSSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRF 461 Query: 424 PATQTI 429 P + Sbjct: 462 PGRSAV 467 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 111 bits (278), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 76/234 (32%), Positives = 122/234 (52%), Gaps = 17/234 (7%) Query: 200 QRIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLDHATRTAHLKLMTEQGQRMQNL 259 +R + +RR+F+A+ SHELRTPL ++NG LE AL L +T L+ + + + + L Sbjct: 242 ERNEKLRREFMADVSHELRTPLAILNGQLE-ALEDGVLQPDAQT--LRSLKSEVEHLNKL 298 Query: 260 IDDMLTLTRLESIDYPLRSEVLRVRPLL--------ERIAEEGRALSAGKHDIRLIVDGP 311 + D+ L+ ++ R + + PLL +R ++ G LS L P Sbjct: 299 VGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSC-----ELPASMP 353 Query: 312 DLKGSADELRSAFTNLVTNAVRYTPEGGTITLRWESDEEGAHFSVQDTGIGISPEHISRL 371 + G A L+ +NL N++RYT GG + + ++ H D+ G+ PE ++RL Sbjct: 354 -VHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVPPEVMARL 412 Query: 372 TERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLDIASVPDHGSTFTVHFPA 425 ERF+RV+ SR+R + G GLGLAI R ++ H T+ ++ P G V PA Sbjct: 413 FERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPA 466 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 84/234 (35%), Positives = 118/234 (50%), Gaps = 24/234 (10%) Query: 206 RRDFVANASHELRTPLTVINGFLEIALAQPNLDHATRTAHLKLMTEQGQRMQNLIDDMLT 265 +R FVA+A+HELRTPLT + L+ LA+ +D R A + +R +L+ +LT Sbjct: 216 QRAFVADAAHELRTPLTALR--LQTQLAERAVDEEERRAAFADLKNGLERTTHLVHQLLT 273 Query: 266 LTRLES-----IDYPLRSEVLRVRPLLERIAEEGRALSAGKHDIRLIVDGPD-----LKG 315 L R E LR+ + V L +AE G D+ L G D + G Sbjct: 274 LARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHG------GIDLGLSDQGDDGRPLRVTG 327 Query: 316 SADELRSAFTNLVTNAVRYTPEGGTITLRWESDEEG-AHFSVQDTGIGISPEHISRLTER 374 +AD LR F NLV NA+RYTP GG + + + G VQD+G GI+ + R+ +R Sbjct: 328 NADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDR 387 Query: 375 FYRVDKSR----SRETQGTGLGLAIVRHVLLRHHATLDIASVPDHGSTFTVHFP 424 FYR S+ SR G+GLGLAIVR + H +++ + HG V FP Sbjct: 388 FYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNT-GHGLQARVVFP 440 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 80/234 (34%), Positives = 125/234 (53%), Gaps = 20/234 (8%) Query: 205 MRRDFVANASHELRTPLTVINGFLEIALAQPNLDHATRTAHLKLMTEQGQR-MQNLIDDM 263 +++ FVA+A+H+LRTPL VI+ ++ A LD R A + ++G R M +L + + Sbjct: 236 VQKRFVADAAHQLRTPLAVIDSQIQFAR---QLDDPARVAQVLASLQEGSRSMTDLTNKL 292 Query: 264 LTLTRLESID---YPLRSEV--LRVRPL-LERIAEEGRALSAG-KHDIRLIVDGPDLKGS 316 L L++ E+ D +P R+ V L V + LE ++ G AL G + ++ + G+ Sbjct: 293 LLLSQAEASDSSAFP-RAPVDLLAVSAMVLEELS--GLALKKGIDLGLETTLESAMVNGN 349 Query: 317 ADELRSAFTNLVTNAVRYTPEGGTITLRWESDEEGAHFSVQDTGIGISPEHISRLTERFY 376 +L NL+ NA+RYTPEGG +T+ E +V D G GI E R+ ERFY Sbjct: 350 EAQLMGMLMNLIDNALRYTPEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFY 409 Query: 377 RVDKSRSRETQGTGLGLAIVRHVLLRHHATLDIASVPDHGSTF--TVHFPATQT 428 R + + + G+GLGLAIVR ++ ++ + P GS TV PA Q+ Sbjct: 410 R---NAAPDQPGSGLGLAIVREIVQASQGSIRL-DAPAQGSGLVATVSLPALQS 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,400 Number of extensions: 54700 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 39 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)