BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1058.p (552 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (... 831 0.0 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 393 e-110 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 377 e-105 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 367 e-102 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 358 e-100 >gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (serine chemoreceptor) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 574 Score = 831 bits (2146), Expect = 0.0, Method: Compositional matrix adjust. Identities = 469/575 (81%), Positives = 502/575 (87%), Gaps = 24/575 (4%) Query: 1 MMQHLKIGSRLAFGFFLILALATAILVIGLWRMAELEASNEYLIEKKVTAMTAALNMREA 60 M+Q LKIGSRLAFGF LIL LATAIL+IGLWRMAELEA++EY+I++KV AMTAALNMREA Sbjct: 1 MIQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREA 60 Query: 61 GSALALALRKVVTPTDAAEGKTEDAHLGKILQAYAGYEKQLTDLSPGGRGKELLLATSGE 120 GS LALALRKVVTPTDAAEGK EDAHL KILQAYAG+EKQ+T+LSP GRGKELL A SG+ Sbjct: 61 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQ 120 Query: 121 GKKLFPLVGQIRQQVGSNNYFDAAQQLKSDFLPAHEKWMSSVAALASFQQEDMQQAHAQA 180 K+LFP+V IRQQV SNNYFDAAQ LKSDFLPAH+KWM+SV ALA+FQQEDM+ AHA+A Sbjct: 121 SKQLFPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEA 180 Query: 181 RDSYRKAQIGMLAMGVLTLGLGCFFTFVITRSIVTPLKRAGQIAETISRGDLTQHAQAQG 240 +DSYRKAQIGML MGVLTLGLG FFTFVITRSIV PLKRAG IAETISRGDLTQ G Sbjct: 181 KDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVGPLKRAGNIAETISRGDLTQDFHDHG 240 Query: 241 HDEAAHLVNTLDAMQANLAATLNEVKQSAAVIAVASHEIARGNVDLSNRTESQASSLEET 300 DEAA +VN L+AMQ NLAATLNEVK+SAAVIAVASHEIARGNVDLSNRTESQASSLEET Sbjct: 241 RDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNRTESQASSLEET 300 Query: 301 ASSMEQLTSTVQQNAANAQQANQLVLSASDHATKGGRVVGDVVTTMGSIKESSGKIVDII 360 ASSMEQLTSTVQQNA NA+QANQLV+SASD+ATKGGRVVGDVV TMGSIKESS KIVDII Sbjct: 301 ASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSIKESSSKIVDII 360 Query: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLISDSV 420 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIK LISDSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKGLISDSV 420 Query: 421 DKVDTGGKLVDEAGATMSEIVASVKQVADIMGEITAASQEQSAGIAEVNHAITQIDEITQ 480 +KVD GGKLVDEAGATMSEIV SVK VADIMGEITAASQEQSAGIAEVNHAITQIDEITQ Sbjct: 421 EKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVNHAITQIDEITQ 480 Query: 481 QNAALVEQAAAAAVSLQEQADVLAQAVAVFKLKTVLQVPTV---------PVPLRSVNAP 531 QNAALVEQAAAAA SLQEQADVLA+AVAVF+LKT ++ P PLR+VN P Sbjct: 481 QNAALVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPAPPAPLRTVNTP 540 Query: 532 PAVTAAAKP--------------ARLARDEEFEEF 552 AVT A +P A L RD+EFEEF Sbjct: 541 AAVT-ATRPVASLPAAAPAKRKPAALTRDDEFEEF 574 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 393 bits (1010), Expect = e-110, Method: Compositional matrix adjust. Identities = 257/537 (47%), Positives = 330/537 (61%), Gaps = 30/537 (5%) Query: 2 MQHLKIGSRLAFGFFLILALATAILVIGLWRMAEL-EASNEYL---IEKKVTAMTAALNM 57 M++LKIG RLA GF +L L ++ +G+WR+ + +A+++ ++K+ A+ + Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 58 REAGSALALALRKVVTPTDAAEGKTEDAHLGKILQAYAGYEKQLTDLSPGGRGKELLLAT 117 +E G ++ A K DA +I Q E +TD Sbjct: 61 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITD-------------- 106 Query: 118 SGEGKKLFPLVGQIR--QQVGSNNYFD---------AAQQLKSDFLPAHEKWMSSVAALA 166 E K+LF VG++R + + FD A + + + + + V A Sbjct: 107 -AEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYA 165 Query: 167 SFQQEDMQQAHAQARDSYRKAQIGMLAMGVLTLGLGCFFTFVITRSIVTPLKRAGQIAET 226 FQ++ + Q A YR + +LA+G + + LG +++TRSI PL +A IAET Sbjct: 166 DFQKQVIDQTAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAET 225 Query: 227 ISRGDLTQHAQAQGHDEAAHLVNTLDAMQANLAATLNEVKQSAAVIAVASHEIARGNVDL 286 ++ GDLT + DE L+ L M NL + V+ I AS EIA GN+DL Sbjct: 226 VAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDL 285 Query: 287 SNRTESQASSLEETASSMEQLTSTVQQNAANAQQANQLVLSASDHATKGGRVVGDVVTTM 346 S RTE+QA SLEETAS+MEQLTSTV+QNA NA+QAN+L SAS AT+GG VV VV TM Sbjct: 286 SARTEAQAGSLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATM 345 Query: 347 GSIKESSGKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSA 406 G+I SS KI DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA Sbjct: 346 GAIHASSQKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA 405 Query: 407 AAAKEIKQLISDSVDKVDTGGKLVDEAGATMSEIVASVKQVADIMGEITAASQEQSAGIA 466 +AAKEIKQLI DSV+KVD G +LV AG TM E+V SV +V ++ EIT ASQEQS GI Sbjct: 406 SAAKEIKQLIDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIE 465 Query: 467 EVNHAITQIDEITQQNAALVEQAAAAAVSLQEQADVLAQAVAVFKLKTVLQVPTVPV 523 +VN AIT IDE TQQNAALVEQAAAAA S+Q+QA L AV+VF+L+ QV V V Sbjct: 466 QVNLAITHIDETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAV 522 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 377 bits (968), Expect = e-105, Method: Compositional matrix adjust. Identities = 254/524 (48%), Positives = 332/524 (63%), Gaps = 30/524 (5%) Query: 4 HLKIGSRLAFGFFLILALATAILVIGLWRMAELEASNE---YLIEKKVTAMTAALNMREA 60 +L I +LA F +++AL L+ GL+ + EL+ N+ ++ K + M AA ++ Sbjct: 6 NLNIAKKLALSFAVVIALT---LLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ-- 60 Query: 61 GSALALALRKV-----VTPTD-AAEGKTEDAHLGKILQAYAGYEKQLTDLSPGGRGKELL 114 GS L L ++ VTP + AA K L + + A +E+++++ + + L Sbjct: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 Query: 115 LATSGEGKKLFPLVGQIRQ--QVGSNNYFDAAQQL-KSDFLPAHEKWMSSVAALASFQQE 171 K L V Q ++ +N +D + L D + + ++ A+ Sbjct: 121 -------KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTS 173 Query: 172 DMQQAHAQARDSYRKAQ---IGMLAMGVLTLGLGCFFTFVITRSIVTPLKRAGQIAETIS 228 QA +A +R AQ +G+LA VL + F++ ++ PLK A QIA+ ++ Sbjct: 174 GSAQADEEASTVFRGAQRWIVGLLAATVL---IASLLAFLVATNVSRPLKEAVQIAQRVA 230 Query: 229 RGDLTQHAQAQGHDEAAHLVNTLDAMQANLAATLNEVKQSAAVIAVASHEIARGNVDLSN 288 RGDLT + G DE L+ L AM +L ++EV+Q I ASHEIARGN+DLSN Sbjct: 231 RGDLTLRIRPAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSN 290 Query: 289 RTESQASSLEETASSMEQLTSTVQQNAANAQQANQLVLSASDHATKGGRVVGDVVTTMGS 348 RTE QA SLEETAS+ME+LTSTV QNA NA+QANQL +ASD A++GG VVG VV TM Sbjct: 291 RTEQQAGSLEETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEG 350 Query: 349 IKESSGKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAA 408 I ESS +I DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA A Sbjct: 351 ITESSRRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGA 410 Query: 409 AKEIKQLISDSVDKVDTGGKLVDEAGATMSEIVASVKQVADIMGEITAASQEQSAGIAEV 468 AKEIK+LI DSV KV G LV+ AG+TM+E+VASV++V D++ EI+AA+QEQS GI EV Sbjct: 411 AKEIKELIDDSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEV 470 Query: 469 NHAITQIDEITQQNAALVEQAAAAAVSLQEQADVLAQAVAVFKL 512 N AITQ+DE TQQNAALVEQAAAAA SL+ QA LA V FKL Sbjct: 471 NLAITQMDETTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 367 bits (942), Expect = e-102, Method: Compositional matrix adjust. Identities = 236/517 (45%), Positives = 332/517 (64%), Gaps = 8/517 (1%) Query: 2 MQHLKIGSRLAFGFFLILALATAILVIGLWRMAELEASNEYLIEKKVTAMTAALNMREAG 61 ++ +KI RLA F +++ + I +G+ ++ + S+ ++E + + A+ +R+ Sbjct: 3 LKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNV 62 Query: 62 SALALALRKVVTPTDAAEG----KTEDAHLGKILQAYAGYEKQLTDLSPGGRGKELLLAT 117 + A LR + + E A+ K +A EK L+ RG+EL + Sbjct: 63 NGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILST----ARGRELFQSI 118 Query: 118 SGEGKKLFPLVGQIRQQVGSNNYFDAAQQLKSDFLPAHEKWMSSVAALASFQQEDMQQAH 177 ++R+ + +A + L + +PA +K+ + +FQ+ M+Q+ Sbjct: 119 QEARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSV 178 Query: 178 AQARDSYRKAQIGMLAMGVLTLGLGCFFTFVITRSIVTPLKRAGQIAETISRGDLTQHAQ 237 + RD+ + A ML + + + L +++TRSI PL A +A ++ GDLT Sbjct: 179 TEGRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIG 238 Query: 238 AQGHDEAAHLVNTLDAMQANLAATLNEVKQSAAVIAVASHEIARGNVDLSNRTESQASSL 297 A DE L+ L +M +L ++EV+Q + I AS EIA GN+DLS+RTE QA +L Sbjct: 239 ATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGAL 298 Query: 298 EETASSMEQLTSTVQQNAANAQQANQLVLSASDHATKGGRVVGDVVTTMGSIKESSGKIV 357 EETAS+ME+LTSTV+QNA NA+QAN L +AS+ A +GG VVG VV TMG I ++S KIV Sbjct: 299 EETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIV 358 Query: 358 DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLIS 417 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA+AAKEIK LI Sbjct: 359 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALID 418 Query: 418 DSVDKVDTGGKLVDEAGATMSEIVASVKQVADIMGEITAASQEQSAGIAEVNHAITQIDE 477 DSV +VD G +LV++AG TM+E+VASV++V D++ EI+AAS EQS GI ++NHAITQ+D+ Sbjct: 419 DSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQ 478 Query: 478 ITQQNAALVEQAAAAAVSLQEQADVLAQAVAVFKLKT 514 +TQQNAALVE+AAAAA SLQEQ+ L+ V+VFKL + Sbjct: 479 VTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKLSS 515 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 239/549 (43%), Positives = 337/549 (61%), Gaps = 9/549 (1%) Query: 2 MQHLKIGSRLAFGFFLILALATAILVIGLWRMAELEASNEYLIEKKVTAMTAALNMREAG 61 +L I +RLA F +++ + I+ +GL R+A++ + + ++ + + + Sbjct: 3 FNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKV 62 Query: 62 SALALALRKVVTPTDAAEGKTEDAHLGKILQAYAGYEKQLTDLSPGGRGKELLLATSGEG 121 +A++LR + TD A+ E + + +LT L K L + Sbjct: 63 DDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTETR 122 Query: 122 KKLFPLVGQIRQQVGSNNYFDAAQQLKSDFLPAHEKWMSSVAALASFQQEDMQQAHAQAR 181 K L QI + S + A + + S+ +P + + S + L+ QQ+ M Q+ QA Sbjct: 123 KSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQAE 182 Query: 182 DSYRKAQIGMLAMGVLTLGLGCFFTFVITRSIVTPLKRAGQIAETISRGDLTQHAQAQGH 241 +Y ++ M+ GVL F + I+RSI PL RA ++AET++ GDLT A + Sbjct: 183 AAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAHSN 242 Query: 242 DEAAHLVNTLDAMQANLAATLNEVKQSAAVIAVASHEIARGNVDLSNRTESQASSLEETA 301 DE L+ L AM L A + EV++ + AS +IA GN+DLS+RTE QAS+LEETA Sbjct: 243 DETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEETA 302 Query: 302 SSMEQLTSTVQQNAANAQQANQLVLSASDHATKGGRVVGDVVTTMGSIKESSGKIVDIIG 361 SS+E+LTS+V+QNA + +++++L +AS A +GG V VV TMG+I +SS KIVDII Sbjct: 303 SSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDIIS 362 Query: 362 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLISDSVD 421 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA AAKEIK+LI+DSV+ Sbjct: 363 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELINDSVN 422 Query: 422 KVDTGGKLVDEAGATMSEIVASVKQVADIMGEITAASQEQSAGIAEVNHAITQIDEITQQ 481 +V +G +LV +AG+TM +V S++QV+ I+GEI+AA++EQS GI +VN AI Q+D TQQ Sbjct: 423 QVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNTTQQ 482 Query: 482 NAALVEQAAAAAVSLQEQADVLAQAVAVFKLKTV--------LQVPTVPVPLRSVNAPPA 533 NAALVE+AAAAA +LQ+QA L V VF+L V P PV LR V+A Sbjct: 483 NAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEVGGSGPAPSAVAPVAPV-LRKVSAAAP 541 Query: 534 VTAAAKPAR 542 + PA+ Sbjct: 542 RATKSLPAK 550 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.331 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,152,237 Number of extensions: 41578 Number of successful extensions: 516 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 285 Number of HSP's successfully gapped: 71 Length of query: 552 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 462 Effective length of database: 1,193,720 Effective search space: 551498640 Effective search space used: 551498640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)