BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.1021.p
         (566 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion ...   603   e-173
gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ...    57   5e-09

>gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 565

 Score =  603 bits (1554), Expect = e-173,   Method: Compositional matrix adjust.
 Identities = 308/533 (57%), Positives = 402/533 (75%), Gaps = 1/533 (0%)

Query: 35  PPDAGTILQQLQPALPAPPASSAPSLKLEPRAESALPQSAPFEVKRLQITGNTVFATESL 94
           P DAG++LQ+LQ A    PA + P LK++    S LP SAPF VK ++I GNT F   +L
Sbjct: 33  PTDAGSLLQELQTAPRTAPAPNQPELKIQSPDASQLPPSAPFLVKEIRIVGNTAFDAATL 92

Query: 95  HELVAGEEGKSLTLAQVNTLAERITRYYHQQGYPLARAIIPAQTIRDGVVVIQVVEARYG 154
           H LVA +EGK+LTLA ++ LA RI+ YY   G+PLARAIIPAQTI +GVVVIQVVEARYG
Sbjct: 93  HALVADQEGKTLTLADLSALAARISSYYQAHGFPLARAIIPAQTISEGVVVIQVVEARYG 152

Query: 155 QVRLNNQSAVRDGLLQATLDGLQSGAVIEDDALNRSLLLLSDVPGVAVAATLQPGASVGS 214
           ++ LNN S V   LL++ L  LQ   ++ + +L+RSLLLLSDVPGV V A L+PG +VG+
Sbjct: 153 KIHLNNSSPVNSRLLESILSSLQKDQMVAERSLDRSLLLLSDVPGVGVEAVLKPGEAVGT 212

Query: 215 SDVTVNTTTNPTTLGTVALDNYGNRYIDRVRVSGNLNVVNPLRHGDLLSATVLSTGSGMN 274
           SD+ V TT    ++G++++DN+GNRYI R R+SG  ++ NPL HGD+LSA ++STG  M+
Sbjct: 213 SDMDVVTTPKAVSIGSMSIDNFGNRYIGRARLSGTASLFNPLHHGDVLSANLVSTGERMS 272

Query: 275 YGRLSYDILVNGQGTHIGTAYSYLRYKLGSELSALGANGTASVVSLWGRHPLLRSREANV 334
           YGR+SYD L+NGQGTH+G AYS + YKLG  + AL A+G A V SLW +HPLLRSR+AN+
Sbjct: 273 YGRISYDTLLNGQGTHVGGAYSMVHYKLGDSVRALDAHGNALVASLWLKHPLLRSRDANL 332

Query: 335 YAQLQLDGKRLHDHVDASALRTDRHLLNWVMSLNGDIRDNLLAGGISIWSLGWTSGRVFF 394
           Y Q+Q D K+L D + A+ LRTDRHL NWV+SL+G++RD++L GG+S W +GWT+GRV +
Sbjct: 333 YGQIQYDTKKLEDRIGATGLRTDRHLDNWVLSLSGNLRDSVLGGGVSAWGMGWTAGRVGY 392

Query: 395 DDAAAASADAAAGQTRGGFAKWNANFSRLQGISQRDAVYLNLAAQWSDRNLDSAEKMTAG 454
           DDAAA ++DA   QTRGGF+KWN NFSRLQ + +RD++Y N + QWSD NLDSAEKM+ G
Sbjct: 393 DDAAAEASDALTAQTRGGFSKWNFNFSRLQFLGRRDSLYANFSVQWSDANLDSAEKMSVG 452

Query: 455 GPYTVRAYDIGAMSGDIGYVGTIEWRHELGNAWSGQWQAIAFLDSAYVKVNQRQWTSGEN 514
           GPY+VRAYDIGA+SGD GY+G+IE RHE+G+   G  Q +AF+DSA V +N+RQWT+G+N
Sbjct: 453 GPYSVRAYDIGAISGDTGYLGSIELRHEMGSMAGGTLQVLAFVDSARVNINRRQWTTGQN 512

Query: 515 SGSLSGAGVGMNWSGPDQWRASVSVATRVG-ARPSIVASQPSVRGWVTLSKAF 566
           S +LSG GVGM WS    W AS  VAT++G A  S++ S  S R W+ +SK F
Sbjct: 513 SVTLSGTGVGMRWSNDALWEASAYVATKIGSANSSLIESSASTRAWLVVSKGF 565


>gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 569

 Score = 56.6 bits (135), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 39/162 (24%), Positives = 67/162 (41%)

Query: 76  FEVKRLQITGNTVFATESLHELVAGEEGKSLTLAQVNTLAERITRYYHQQGYPLARAIIP 135
           FE++R  + GNT+   + +   VA   G+      +    E +   Y   GY   +  +P
Sbjct: 53  FEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVHVP 112

Query: 136 AQTIRDGVVVIQVVEARYGQVRLNNQSAVRDGLLQATLDGLQSGAVIEDDALNRSLLLLS 195
            Q +  G V I VVE   G+V+++         ++A++  L+ G       ++ S+ L +
Sbjct: 113 EQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQLAN 172

Query: 196 DVPGVAVAATLQPGASVGSSDVTVNTTTNPTTLGTVALDNYG 237
           D P   V  TL    S    D  +    +      + LDN G
Sbjct: 173 DNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTG 214


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.131    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,675,021
Number of extensions: 69705
Number of successful extensions: 263
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 263
Number of HSP's successfully gapped: 2
Length of query: 566
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 476
Effective length of database: 1,193,720
Effective search space: 568210720
Effective search space used: 568210720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 133 (55.8 bits)