BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1020.p (1634 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310333|ref|YP_003774425.1| filamentous hemagglutinin outer... 1473 0.0 gi|300309649|ref|YP_003773741.1| hemagglutinin-like protein [Her... 110 1e-24 >gi|300310333|ref|YP_003774425.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 1568 Score = 1473 bits (3813), Expect = 0.0, Method: Compositional matrix adjust. Identities = 916/1674 (54%), Positives = 1150/1674 (68%), Gaps = 146/1674 (8%) Query: 1 MR-HASMNRIYRLVWSHVQQAWVIVSERTRGQGKKVSRRLAAAAALSVASALAMAGPSGG 59 MR HAS+NRIYRLVWSHV QAWV+VSE +RGQGK+ +R++ AAA L V+ LA A P GG Sbjct: 1 MRGHASLNRIYRLVWSHVHQAWVVVSEVSRGQGKRANRKVIAAALL-VSPLLAQAAPVGG 59 Query: 60 AVTAGSGSISQSGNTTTITQGSQNLSVNWQSFNTTSAEKVNFVQPSTSAIAVNRIQDVNA 119 VTAG+GSISQSGNTTTITQ SQNLSVNWQSFNT S E VNF+QPS SAIAVNRI D N Sbjct: 60 QVTAGAGSISQSGNTTTITQSSQNLSVNWQSFNTASHETVNFIQPSASAIAVNRIYDTNP 119 Query: 120 TQFMGQLNANGQVYLINPNGILFGAGAQVNVGGLVASTLDINDAALGSSTRNFSGMGSGS 179 T+F GQLNANGQVYLINPNG+LFGAG+QVNVGGLVASTLD++DA+L ++ RNFSG G+GS Sbjct: 120 TRFFGQLNANGQVYLINPNGVLFGAGSQVNVGGLVASTLDMSDASLAAAARNFSGSGTGS 179 Query: 180 IVNQGSINSANGGYVAFLGKQVANQGSITTTAGTTALAAGSDVTLSFAGNSLVSLRVNQS 239 I+NQG I+ ANGGYVAF+G V+NQG+IT AGT ALAAG+DVTL+FA SLV L+VNQ+ Sbjct: 180 IINQGDISVANGGYVAFIGNTVSNQGNITAPAGTVALAAGNDVTLTFANASLVKLQVNQN 239 Query: 240 TLDSLSGNGGLIQADGGTVLLSAGARDTVLASVVNNTGIIQARSVQNVNGTIVLEAGSHG 299 TL++LS NGGLI ADGG VL+SAGA+D VLASVVNNTGII+ARSVQNVNG IVL+ G+ Sbjct: 240 TLNNLSENGGLILADGGAVLMSAGAKDAVLASVVNNTGIIEARSVQNVNGRIVLDGGNLA 299 Query: 300 TATNSGTLDASGRNAGETGGTVKLLGGTVAQTSGGRIDVAGDAGGGTAYVGGNFLGAGPE 359 T +NSGTLDASG+NAG+TGGTVK+LG V SG ID++GDAGGG+A+VGGNFLGAGPE Sbjct: 300 TVSNSGTLDASGKNAGQTGGTVKVLGKEVTLASGSNIDISGDAGGGSAFVGGNFLGAGPE 359 Query: 360 QNATTTTVEAGSSIDASAVNSGNGGRVALWSDGTTRFDGSITARGGAASGNGGAVETSGL 419 QNA TTV AG++I+A A+++GNGG VA+WSDG T F+GSITARGG GNGG VETSG Sbjct: 360 QNALNTTVAAGATINADALSNGNGGNVAIWSDGNTSFNGSITARGGKDGGNGGQVETSGE 419 Query: 420 KLKIGSTASVNTLAPRGSAGNWLLDPADIKIGSIAFWGS--NYGIDVDSAVLTAALNNGN 477 L +G A VNTLAP+G G+WLLDP D++I ++ N D+ A +TAAL + Sbjct: 420 HLTVGKPAFVNTLAPKGKVGSWLLDPQDMRI----YYDQDPNSHGDISGAQITAALQYSD 475 Query: 478 VTIKTSRSGPSCGVA----CGSGSGSSGDILVLDPIGVAVASYDANHTATYVNWDSNSYT 533 VTIKT GP+ CG+G+ +GDI ++D GV + +NW + + T Sbjct: 476 VTIKT---GPTVSCTNVSGCGAGTSGNGDINLVD--GVLIGG---------MNWTTGT-T 520 Query: 534 LTLSAYNNVRFVFTPNYVSVGAGTSQSSGGAVDMSLGHGTKTILQADNSAKGSGTVVFDD 593 LTLSAY ++ + +D + G+G +L+ADN+ G+G+V FD Sbjct: 521 LTLSAYRDINLGYAT---------------FIDATGGNG-NVVLRADNTGTGTGSVKFDA 564 Query: 594 -------NNGWTYIGLGDAATTAKIYYTPTSYSAPQDFTPYLIGYNQLT---QLTSYMAV 643 NNG+T KIYY P Y P DF+ ++ +T Q +YMA+ Sbjct: 565 NALGVQGNNGYT-----------KIYYNPDDYLHPTDFSLFV----TMTGSGQFNAYMAI 609 Query: 644 NVAASIASKTYDGTLGATISSVSTVASLPGGMSPLSTSGATASFADKNAGSNKDVLLSGV 703 N+ A I SKTYDGT AT+S S P G++ ++TSGA A+F D+ AG+NK V +SGV Sbjct: 610 NLTAGIVSKTYDGTTTATLSGAVNPISTPAGVT-INTSGAIATFNDRFAGTNKAVTISGV 668 Query: 704 TFSNGSDTINVGGENYYLNGLSTQLGTITPKSVDVT-GLSVVSKTYDGTTAATLSGTAGF 762 T GS+ Y+LNGL ++ TI ++ + GL+ KTY+GTT AT+SGTA Sbjct: 669 TLG-GSE-----AGKYFLNGLDSKTATINKANLTLNGGLTANDKTYNGTTTATISGTASV 722 Query: 763 SGLVGGDSLSLNLSSATANFADANAGTKAVTLGSYTLGGSSSANYVLNQPS-LTGTINKA 821 + L S S+++S ++ANF+D N GT +Y L GS S NY L QP+ LT INKA Sbjct: 723 AAL---GSDSVSVSLSSANFSDKNVGTNKAVTATYVLTGSDSGNYNLIQPTGLTANINKA 779 Query: 822 NLSLSG----SKTYDGTTDIDGSQLTATGVHGETFTVAGSG------SSGNLASKNVQSG 871 N++++G SKTYDGTT TAT V G T GS SS N + KN S Sbjct: 780 NVTVTGATANSKTYDGTT-------TAT-VSGATVAAFGSDVVSLALSSANFSDKNAASN 831 Query: 872 AALASVTGLSLGSSSNGGLASNYNALGTSGSSISVTAKALTMSGVTASDKVYDGSTTATL 931 + + L+ G A+NYN + +G + ++ LT+ G++A+DK Y TT Sbjct: 832 KGVTATYTLT------GTDANNYNLIQPTGLTATINKANLTVGGISAADKTY--DTTTAA 883 Query: 932 NTSGVGFFGIIGGDDVSLGGSGVGSFANKNVGTNKAVTVSGYTLSGADAGNYLISQPTGL 991 +G +G D VS+ G+GVG+F+NKN G+NK VTVSGYTL GADAGNY I QPTG+ Sbjct: 884 TLTGTASVVALGSDVVSVSGTGVGTFSNKNAGSNKVVTVSGYTLGGADAGNYNIVQPTGV 943 Query: 992 SASITKADLSVSGISAQGKTYDGQTTATLSGTASVTAIGSDVVSLGGTGVGVFIDKNAGV 1051 +A+I KA+L+VSGI+A KTYD T ATL+GTASVTA+GSDVVS+ G+GVG F +KNAG Sbjct: 944 TATINKANLNVSGITANNKTYDATTVATLAGTASVTALGSDVVSVTGSGVGSFANKNAGN 1003 Query: 1052 NKVVNVSGYTLSGSDAGNYNLVQPTGVSATISKANLTLSGLTANNKTYDGTIVAALSGTA 1111 K V VSGY+L+GSDAGNYN+VQPTG+ ATISKANL + G++A++KTYD T A L+G A Sbjct: 1004 AKAVTVSGYSLTGSDAGNYNIVQPTGLVATISKANLAVGGISASDKTYDATTTATLTGVA 1063 Query: 1112 SVTALGSDVVSVTGTGAASFADKNVGTGKGVTVSGYTLTGTDAGNYNLVSPGGLSADISK 1171 SV ALGSDVVSV+G G +F+DKN G+ K VTV+GYTL G DAGNYN++ P GL+A I+K Sbjct: 1064 SVAALGSDVVSVSG-GVGTFSDKNAGSNKAVTVTGYTLGGADAGNYNVIQPTGLTATINK 1122 Query: 1172 ASLTVGGVSAQSRAYDGTTVATVAGTASVSAFGADVVSVAGTASASFADKNVGSNKAVLV 1231 ASLTVGG+SA + YD TTVAT+ GTA+V+A G+DVVS+ GT +FA+KN G+NKAVLV Sbjct: 1123 ASLTVGGLSAADKTYDTTTVATLTGTATVAALGSDVVSLTGTGVGAFANKNAGANKAVLV 1182 Query: 1232 SGYTLTGADAGNYDVVQPSGLNATISKADLAVAGLSVANKIYDGTTTATLTGTGSVTALG 1291 SGY+LTG+DA NY++VQPSGL+ATI+KADL V G+S A+K YD TT ATL+GT SV+ALG Sbjct: 1183 SGYSLTGSDASNYNIVQPSGLSATINKADLHVTGISAADKTYDATTAATLSGTASVSALG 1242 Query: 1292 SDVVTLSGSGAASFANKNVGTGKSIVISGYTLGGTDGGNYNLVAPSNLTADITPLQITVT 1351 SDVV L G+G +F+NKN G+ K++ ++GYTL GTD GNYN+V P+ LTA I+ +T Sbjct: 1243 SDVVALGGAGVGNFSNKNAGSNKAVTVTGYTLTGTDAGNYNVVQPTGLTASISKANLTAG 1302 Query: 1352 GIVANNKTYDGTVAATANTSGVSLAGKLAGDVINVAATGSFSDKNVGSNKAVTLNSIYSG 1411 + NNK YDGT AAT SG + L D ++V A G FSDKNVG+ K VT+ SG Sbjct: 1303 SVSVNNKVYDGTTAATV--SGTASVTALGSDSVSVIAGGVFSDKNVGNGKTVTVTYTLSG 1360 Query: 1412 ADAGNYTYIDQTSSQASITPASLNVSGITAANKVFDGTTAATINTGSVVLGGLIGGDNVR 1471 DAGNY I + A+ITPASL VSGITA +K FDGTT A++N +L G + GD V Sbjct: 1361 TDAGNYNVIQPAAQTANITPASLTVSGITANDKPFDGTTTASVNAAGAILTGKVAGDVVT 1420 Query: 1472 FSSSGAFSDAAIGRGKTVNLSNSISGADSGNYTLIGQSTALADITAAGSAQTPSAVATAP 1531 +++G FSD A+G GK VNLS++ GAD GNYT+ Q+T A IT+ A P Sbjct: 1421 VAATGVFSDPAVGNGKLVNLSSTYGGADVGNYTITDQATTTASITS----------ALPP 1470 Query: 1532 AAPTPSVQAVTVRNVLTQVQSTVLPPQASAQPQSLSLSSTLVVRS-----QQGDA----A 1582 AAP+ SV A VRNVL QVQS+VLPPQA++QPQSL LSSTL VR+ QQ D A Sbjct: 1471 AAPSASVPA-PVRNVLAQVQSSVLPPQAASQPQSLDLSSTLTVRAEGREGQQSDGSANDA 1529 Query: 1583 PATGN--ESSSGNAGGRGGSSLVNTSTGFGTPPPSLNIQNGGIQLPPVAISTPQ 1634 P N E N GG G P L I+NGG QLP A T + Sbjct: 1530 PRRANTIELGGANVGGAG---------------PVLQIRNGGTQLPDNAFRTTE 1568 >gi|300309649|ref|YP_003773741.1| hemagglutinin-like protein [Herbaspirillum seropedicae SmR1] Length = 1422 Score = 110 bits (275), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 102/325 (31%), Positives = 158/325 (48%), Gaps = 31/325 (9%) Query: 8 RIYRLVWSHVQQAWVIVSERTRGQGKKVSRRLAAAAALSVASALAMAG------------ 55 R L W + +++ R + +R LA A +SVA+A A+ G Sbjct: 30 RRLTLSWRARKPLPQLLAGLLR---RSWTRLLAPALRISVATA-AVCGAWQAPVLAAAPA 85 Query: 56 ----PSGGAVTAGSGSISQSGNTTTITQGSQNLSVNWQSFNTTSAEKVNFVQPSTSAIAV 111 P+G +V G+ + Q+GN I Q S + SF+ + VN QPS +A + Sbjct: 86 PGTLPTGWSVVNGNVTFIQNGNILNINQLSPQAIAQFLSFSIGADATVNVNQPSAAAALL 145 Query: 112 NRIQDVNATQFMGQLNANGQVYLINPNGILFGAGAQVNVGGLVASTLDINDAALGSSTRN 171 ++ + +Q G+L+A G V L NPNG++ G ++ G +A++L I+D+ + N Sbjct: 146 AKVSGGDISQIYGKLSATGTVVLYNPNGVVIGPSGSIDAGRFIATSLAISDSDFLAGKLN 205 Query: 172 FSGMGSGSIV-NQGSINSANGGYVAFLGKQVANQGSITTTAGTTALAAGSDVTLSFAGNS 230 F+ G+ IV NQG I SA GG V +G V+N G I + G LAAG VTL+ Sbjct: 206 FARQGNAGIVDNQGKIQSATGGSVYLIGSSVSNSGIIKSAQGEVILAAGETVTLADTATP 265 Query: 231 LVSLRVNQSTLDSLSGNGGLIQADGGTVLLSAGARDTVLASVVNNTGIIQARSVQNVNGT 290 V++ V S + N G I A+ G + ++AG ++NN+G+I A SV G Sbjct: 266 GVTVNVTGSAGNVT--NLGSITAEAGRIGVAAG--------LINNSGVINASSVVREGGR 315 Query: 291 IVLEAGSHGTATNSGTLDASGRNAG 315 I L A + T + S ++ A G G Sbjct: 316 IFLRASQNLTTSASSSIHADGSRGG 340 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.307 0.124 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,206,827 Number of extensions: 167453 Number of successful extensions: 936 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 753 Number of HSP's successfully gapped: 20 Length of query: 1634 Length of database: 1,619,870 Length adjustment: 98 Effective length of query: 1536 Effective length of database: 1,155,840 Effective search space: 1775370240 Effective search space used: 1775370240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 137 (57.4 bits)