BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.1017.p
         (383 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase [Herbas...   336   2e-93
gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate dehydro...   239   3e-64

>gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 380

 Score =  336 bits (861), Expect = 2e-93,   Method: Compositional matrix adjust.
 Identities = 169/379 (44%), Positives = 238/379 (62%), Gaps = 6/379 (1%)

Query: 2   IISSTLDYREAARRRLPRFLFDYIDGGAYAEDTLARNSHDLRQLALRQRVLKEVGQVDLT 61
           +++   D+R  A++R+P+  +DY D G+Y E T   NS DL  L LRQRV   V +    
Sbjct: 3   VMTCVEDFRLLAKKRVPKAFYDYADSGSYTESTYRANSKDLAALKLRQRVAINVDERSTR 62

Query: 62  TTLLGQSLAMPIALSPVGLTGMYARRGEVQAARAATKAGIPFTLSTVSVCSIEEVQGAVD 121
           TT++G  + MP+A++P GLTGM    GE+  A AA K GIPFTLST+S+CSIE+V     
Sbjct: 63  TTMIGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASVTT 122

Query: 122 QPIWFQLYVLKDRGFMKNVLDRAWAAGVRNLVFTVDMPVPGARYRDRHSGMS-GPNAAMR 180
           +P WFQLYV++DRGF+K++++RA AA    LV T+D+ + G R++D  +GMS  P   + 
Sbjct: 123 KPFWFQLYVMRDRGFVKSLIERAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLTLE 182

Query: 181 RYLQAVTHPRWALDVGLLGRPHDLGNVSAYLNKKT---GLEDYVGWLGANFDPSIGWHDL 237
             L   + P WAL    LG     GN++ ++       G++    W  + FDP++ W D+
Sbjct: 183 TLLDLASKPGWALRA--LGGRKTFGNLAGHIKGGEGAGGVQTLSKWTASQFDPTLNWDDV 240

Query: 238 QWIRDAWKGNMILKGILDPVDARQAVSFGANGIVVSNHGGRQLDGVLSTARALPEIASAV 297
            WI+  W G +ILKGILD  DA+ AV  GA+ IVVSNHGGRQLDG +S+  ALP IA AV
Sbjct: 241 AWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIAQAV 300

Query: 298 KGELAILVDSGVRSGLDVVRMLALGADTVMLGRAFIYALAAEGQAGVENLLSLFAKEMQV 357
             ++ +  D G+RSG DV++ +ALGA   M+GRAF+Y+L A G  GV  +L +  KE+ V
Sbjct: 301 GDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGEGVSQMLEIMRKELDV 360

Query: 358 AMTLTGAHKIADINEDSLV 376
           +M LTG   I D+    L+
Sbjct: 361 SMALTGTKDIKDVGPQILI 379


>gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  239 bits (609), Expect = 3e-64,   Method: Compositional matrix adjust.
 Identities = 148/382 (38%), Positives = 210/382 (54%), Gaps = 8/382 (2%)

Query: 4   SSTLDYREAARRRLPRFLFDYIDGGAYAEDTLARNSHDLRQLALRQRVLKEVGQVDLTTT 63
            S  + R  AR+R+P F F+Y++GGA  E +L  N     ++    R L +V        
Sbjct: 35  QSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRRQGRR 94

Query: 64  LLGQSLAMPIALSPVGLTGMYARRGEVQAARAATKAGIPFTLSTVSVCSIEEVQGAVDQP 123
           L GQ +A P  + P G +G+ AR G+V  A AA  AG+PF L+ VS  S+EEV       
Sbjct: 95  LFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRRSGAQ 154

Query: 124 IWFQLYVLKDRGFMKNVLDRAWAAGVRNLVFTVDMPVPGARYRDRHSGMSGPNAAMRRYL 183
           +W Q+Y+ +DR F+ +V  RA AAG+  LV T D  V G R  D  +  S      R  L
Sbjct: 155 VWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARNFSSPRRLDWRNKL 214

Query: 184 QAVTHPRWALDVGLLGRPHDL---GNVSAYL--NKKTGLEDYVGWLGANFDPSIGWHDLQ 238
             + HPRW +D+     PH      N+   L  ++ +        LG +   ++ W D+Q
Sbjct: 215 DVLRHPRWLIDILY---PHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSAALDWADVQ 271

Query: 239 WIRDAWKGNMILKGILDPVDARQAVSFGANGIVVSNHGGRQLDGVLSTARALPEIASAVK 298
           W+R  W G ++LKG++   DA++AV+ G +GIV+SNHGGRQLDG LST   LPE+ +AVK
Sbjct: 272 WLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEVVAAVK 331

Query: 299 GELAILVDSGVRSGLDVVRMLALGADTVMLGRAFIYALAAEGQAGVENLLSLFAKEMQVA 358
           G+L +++D G R G D+V+ +ALGAD V+LGRA  Y LAA GQAG    L +   E+   
Sbjct: 332 GQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRSEVDRV 391

Query: 359 MTLTGAHKIADINEDSLVRATA 380
           + L     I  ++   L R  A
Sbjct: 392 LALLACPDIDQLDASYLRRLQA 413


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,231,082
Number of extensions: 55341
Number of successful extensions: 185
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 183
Number of HSP's successfully gapped: 2
Length of query: 383
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 296
Effective length of database: 1,207,925
Effective search space: 357545800
Effective search space used: 357545800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)