BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1012.p (128 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310544|ref|YP_003774636.1| membrane protein [Herbaspirillu... 197 3e-52 >gi|300310544|ref|YP_003774636.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 126 Score = 197 bits (500), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 99/109 (90%), Positives = 104/109 (95%) Query: 1 MKERTLIAAGALNMFMAVGCGAFGAHGLKKMISEEMLAIWHTAVTYQVMHALGMIAIALL 60 MKERTLIA GALNMF+AVGCGAFGAHGLKKMISEEMLAIWHTAVTYQVMHALGMIA+ALL Sbjct: 1 MKERTLIALGALNMFIAVGCGAFGAHGLKKMISEEMLAIWHTAVTYQVMHALGMIAVALL 60 Query: 61 MPRMNGAALRWAGALMLAGIVIFSGSLYLLALTGTRILGAITPLGGVAF 109 MPR+ GAALRWAG LMLAGIV+FSGSLYLLALTGTR+LGAITP GGV F Sbjct: 61 MPRLGGAALRWAGVLMLAGIVVFSGSLYLLALTGTRMLGAITPFGGVGF 109 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 336,739 Number of extensions: 12003 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 1 Length of query: 128 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 53 Effective length of database: 1,264,745 Effective search space: 67031485 Effective search space used: 67031485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 125 (52.8 bits)