BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0964.p
         (307 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspi...   598   e-172
gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protei...    62   8e-11

>gi|300310491|ref|YP_003774583.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 307

 Score =  598 bits (1542), Expect = e-172,   Method: Compositional matrix adjust.
 Identities = 294/307 (95%), Positives = 304/307 (99%)

Query: 1   MDTLYHLIEQYGLILVFVNVLVEQLGAPVPAYPTLIITGALSASGRYSPALLLLLAVCAA 60
           M+TLYHLIEQYGL++VFVNVLVEQLGAPVPAYPTLI+TGALSA+GRYSPA+LLL+AVCAA
Sbjct: 1   METLYHLIEQYGLLIVFVNVLVEQLGAPVPAYPTLILTGALSAAGRYSPAVLLLVAVCAA 60

Query: 61  LMADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120
           L+ADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI
Sbjct: 61  LLADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPGFASI 120

Query: 121 ASALSGTMGTRRLSFVLWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLAELGHYGMLMIA 180
           ASALSGTMGTRR SF+LWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLAELGHYGMLMIA
Sbjct: 121 ASALSGTMGTRRRSFLLWDAAGSALWAGLGIFLGSLFSSAVDDLLNVLAELGHYGMLMIA 180

Query: 181 VALAAFVANKWWQRRRFRKSLAMARISVDELNTLLEAGNAPTIIDVRSPLSQQDGRIPGA 240
           VALAAFVANKWWQRRRFRKSL MARISVDELNT+LE GNAPTIIDVRSPLSQQDGRIPGA
Sbjct: 181 VALAAFVANKWWQRRRFRKSLEMARISVDELNTMLEEGNAPTIIDVRSPLSQQDGRIPGA 240

Query: 241 VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAALVARQLMRRGYTRVRPLTGGIDAWRA 300
           VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAALVARQLMRRGYTRVRPLTGGIDAWRA
Sbjct: 241 VTISNQEIQTFVFDGPVEGEVVVYCACPNEASAALVARQLMRRGYTRVRPLTGGIDAWRA 300

Query: 301 AGYAIDV 307
           AGYAIDV
Sbjct: 301 AGYAIDV 307


>gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 220

 Score = 61.6 bits (148), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 59/221 (26%), Positives = 105/221 (47%), Gaps = 24/221 (10%)

Query: 1   MDTLYH-------LIEQYGLIL---VFVNVLVEQLGAPVPAYP---TLIITGALSASGRY 47
           +D L H       LIEQYG ++   +F+ V  E     +P  P    L I G   A+G  
Sbjct: 7   LDVLVHVDKYLGVLIEQYGALIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGTFCATGAM 66

Query: 48  SPALLLLLAVCAALMADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARS 107
              LL+ L V AA+  + + Y  G   G+++ +   R  L+P++ +R+T + Y + G ++
Sbjct: 67  DIWLLMTLLVVAAVAGNTLNYWIGGWVGQRVYTHNYR-WLNPEA-LRKTHAFYEKHGGKT 124

Query: 108 LLLAKFIPGFASIASALSGTMGTRRLSFVLWDAAGSALWAGLGIFLGSLFSS--AVDDLL 165
           +++A+F P   + A  ++G        F L++  G+ LW    + LG LF +   V   L
Sbjct: 125 IVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVVGLVVLGYLFGNIPVVQQNL 184

Query: 166 NVLAELGHYGMLMIAVALAAFVANKW--WQRRRFRKSLAMA 204
           N L  +G     ++A      +   W  W++ R ++ ++ A
Sbjct: 185 NTLVLIG-----IVAAVFPIVLGGLWQIWRKLRGKRKVSNA 220


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 914,002
Number of extensions: 35187
Number of successful extensions: 229
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 229
Number of HSP's successfully gapped: 3
Length of query: 307
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 222
Effective length of database: 1,217,395
Effective search space: 270261690
Effective search space used: 270261690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)