BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0945.p (758 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxi... 1459 0.0 gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxi... 878 0.0 >gi|300310478|ref|YP_003774570.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 758 Score = 1459 bits (3778), Expect = 0.0, Method: Compositional matrix adjust. Identities = 731/758 (96%), Positives = 742/758 (97%) Query: 1 MTQHISAAEQLLRDAAREYHRTPTRGKIEVRATKPLSNQRDLSLAYSPGVAYPCLDIEAD 60 MTQ ISAAEQLLRDAAREYHRTP+RGKIEVR TKPLSNQRDLSLAYSPGVAYPCLDIEAD Sbjct: 1 MTQQISAAEQLLRDAAREYHRTPSRGKIEVRPTKPLSNQRDLSLAYSPGVAYPCLDIEAD 60 Query: 61 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE 120 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE Sbjct: 61 PLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIELAE 120 Query: 121 NDPDKLVEIIAALEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA 180 NDPDKLVEIIA+LEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA Sbjct: 121 NDPDKLVEIIASLEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIISSAA 180 Query: 181 LLNGLELVGKDIGAVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL 240 LLNGLELVGKDIG VKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL Sbjct: 181 LLNGLELVGKDIGKVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRPGGL 240 Query: 241 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLTAEMVKTMADKPIILALANPEPEIR 300 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVL+AEMVKTMADKPIILALANPEPEIR Sbjct: 241 DASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLSAEMVKTMADKPIILALANPEPEIR 300 Query: 301 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL 360 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL Sbjct: 301 PELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREIADL 360 Query: 361 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPITDYD 420 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPI DYD Sbjct: 361 AKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIKDYD 420 Query: 421 AYRAELTRFIYQTGMFMQPVFNTAKRAAQRVVYADGEDERVLRAVQFVADEGLAAPILVG 480 AYRAELTRFIYQTGMFMQPVFNTAK A QRVVYADG+DERVLRAVQFVADEGLAAPILVG Sbjct: 421 AYRAELTRFIYQTGMFMQPVFNTAKVAPQRVVYADGQDERVLRAVQFVADEGLAAPILVG 480 Query: 481 RAAAIEAGIKKAGLRLKIGQNLQVIDPASDDRVAQYAAQYQGGSEADVRASSSLLAALMV 540 AAIEAGIKKAGLR+KIGQN+QVIDPA+D RVAQYAAQY+ G EADVRAS SLLAALMV Sbjct: 481 APAAIEAGIKKAGLRIKIGQNVQVIDPATDARVAQYAAQYKDGGEADVRASGSLLAALMV 540 Query: 541 KAGHADAMLCGLDGNYDGHLAHVEKVIGLKDGAQEFAALNAVLLPEQTLFITDTYVNDTP 600 KAG ADAMLCGLDGNYDGHLAHVEKVIGLK GAQEFAALNAVLLP+QTLFITDTYVNDTP Sbjct: 541 KAGDADAMLCGLDGNYDGHLAHVEKVIGLKQGAQEFAALNAVLLPDQTLFITDTYVNDTP 600 Query: 601 DAEQLARIAKAAAEEVARFGVPPKVAFLSHSNHGSSSRPSARKMAAAHEAFKKLAPEVES 660 DAEQLARIA+AAAEEVARFGVPPKVAFLSHSN GSSSRPSARKMAAAHEAFKKLAPEVES Sbjct: 601 DAEQLARIAQAAAEEVARFGVPPKVAFLSHSNRGSSSRPSARKMAAAHEAFKKLAPEVES 660 Query: 661 AGELHGDAALSEKIRHNAGLTDSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI 720 AGELHGDAALSEKIR+NAGL DSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI Sbjct: 661 AGELHGDAALSEKIRNNAGLADSGLTGAANVLVCPNLDAANILFNVLKTSSGQGTTIGPI 720 Query: 721 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA 758 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA Sbjct: 721 LLGAAAPVHVLTPSSTVRRVINMTALAAAKAQDGKSKA 758 >gi|300312657|ref|YP_003776749.1| NADP-dependent malic enzyme oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 770 Score = 878 bits (2269), Expect = 0.0, Method: Compositional matrix adjust. Identities = 456/767 (59%), Positives = 566/767 (73%), Gaps = 17/767 (2%) Query: 1 MTQHISAAE---QLLRDAAREYHRTPTRGKIEVRATKPLSNQRDLSLAYSPGVAYPCLDI 57 M IS E Q LR AA +YH PT GKI V TK L+NQRDLSLAYSPGVA C +I Sbjct: 1 MDSMISKKEEVRQQLRQAALDYHEFPTPGKIAVTPTKLLTNQRDLSLAYSPGVAAACEEI 60 Query: 58 EADPLKAFDYTSRGNLVAVISNGTAVLGLGNIGPLASKPVMEGKGCLFKKFAGVDVFDIE 117 ADPL AF YT+RGNLVAVISNGTAVLGLGNIG LASKPVMEGKG LFKKFAG+DVFDIE Sbjct: 61 VADPLNAFRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGIDVFDIE 120 Query: 118 LAENDPDKLVEIIAALEPTLGGINLEDIKAPECFYIEQKLSARMNIPVFHDDQHGTAIIS 177 + E DPDKL+++IAALEPT GGINLEDIKAPECFYIE+KL +M IPVFHDDQHGTAII Sbjct: 121 VNETDPDKLIDVIAALEPTFGGINLEDIKAPECFYIERKLREKMKIPVFHDDQHGTAIIV 180 Query: 178 SAALLNGLELVGKDIGAVKIAVSGAGAAAIACVEVMIGLGVKRENVYMVDSKGVIYEGRP 237 AA+LNGL++VGKDI VK+ VSGAGAAA+AC+++++ LG EN+Y+ D GV+Y+GR Sbjct: 181 GAAILNGLKVVGKDIKNVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRK 240 Query: 238 GGLDASKQRYAIKTEARTLADVVNGADVFLGCSAPGVLTAEMVKTMADKPIILALANPEP 297 +D K+R+A +TEARTLA+V+ AD+FLG SA GVL EMVK MA +P++LALANP P Sbjct: 241 ELMDPDKERFARETEARTLAEVIPDADIFLGLSAAGVLKPEMVKQMAARPLVLALANPTP 300 Query: 298 EIRPELAKSVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVREI 357 EI PE K+VR D IIATGRSDYPNQVNNVLCFPYIFRGALD GAT IT M++A V I Sbjct: 301 EILPEEVKAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIATVHAI 360 Query: 358 ADLAKAETSAEVSAAYPGKELVFGADYLIPTPFDSRLILKIAPAVAKAAAESGVATRPIT 417 A+LA+AE S V+ Y L FG +Y+IP PFD RL++KIAPAVA+AA SGVA RPI Sbjct: 361 AELAQAEQSDIVATTYGIANLSFGPEYIIPKPFDPRLMIKIAPAVARAAESSGVAARPIQ 420 Query: 418 DYDAYRAELTRFIYQTGMFMQPVFNT---AKRAAQRVVYADGEDERVLRAVQFVADEGLA 474 D DAY +L +F+Y +G FM+P+F A A +R+VYA+GE+ERVLRAVQ + DE LA Sbjct: 421 DMDAYIEKLEQFVYHSGTFMRPIFQVAKKAAEAKKRIVYAEGEEERVLRAVQVIVDENLA 480 Query: 475 APILVGRAAAIEAGIKKAGLRLKIGQNLQVIDPASDDRVAQYAAQY------QGGSEA-- 526 PILVGR +E I+K GLRL+ G + +VI+P D+R Y + +G +E Sbjct: 481 KPILVGRPEVLEQRIQKFGLRLRAGTDFEVINPNFDNRYRDYWQTFLEMSRRKGVTEQYA 540 Query: 527 --DVRASSSLLAALMVKAGHADAMLCGLDGNYDGHLAHVEKVIGLKDGAQEFAALNAVLL 584 ++R +L+ ++ + G AD M+CG G HL ++++V+G ++G +AA+NAV+L Sbjct: 541 KLEMRRRHTLIGSMAIHKGDADGMICGTYGTTQLHLHYIDQVLGKREGVNVYAAMNAVIL 600 Query: 585 PEQTLFITDTYVNDTPDAEQLARIAKAAAEEVARFGVPPKVAFLSHSNHGSSSRPSARKM 644 P + L + DT+VN+ P+A +LA I AAEE+ RFG+ P+ A LSHSN GSS+ SARKM Sbjct: 601 PNRQLVMVDTHVNENPNARELAEITMLAAEEMRRFGLHPRAALLSHSNFGSSNSESARKM 660 Query: 645 AAAHEAFKKLAPEVESAGELHGDAALSEKIRHNAGLTDSGLTGAANVLVCPNLDAANILF 704 A +++APE+E GE+HGD AL K+ NA + DS L G AN+LV PN+DAANI + Sbjct: 661 REALAILREIAPELEVDGEMHGDTALDSKLL-NAVIPDSPLKGDANLLVMPNIDAANIAY 719 Query: 705 NVLKTSSGQGTTIGPILLGAAAPVHVLTPSSTVRRVINMTALAAAKA 751 N+LKT+SG G IGPILLG A PVH+LTPS+TVRR+INMTAL A Sbjct: 720 NLLKTASGNGVAIGPILLGCAKPVHILTPSATVRRIINMTALCVMDA 766 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,087,995 Number of extensions: 80885 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 2 Length of query: 758 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 665 Effective length of database: 1,179,515 Effective search space: 784377475 Effective search space used: 784377475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)