BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0941.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 743 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 370 e-103 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 341 7e-95 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 743 bits (1918), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/391 (92%), Positives = 370/391 (94%), Gaps = 2/391 (0%) Query: 9 QHDAASVGSHRAHWASLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVA 68 QHD ++ HRAHWA LRRDLHAHPELRFEEHRTA+VVV+ELQ+LGY VTRGLGGTGVVA Sbjct: 7 QHD--TLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVA 64 Query: 69 SLPGADPQRGIVLRADLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQM 128 SL GADPQRGIVLRADLDALPI EAN+FAHASC+HG+MHACGHDGHTVMLLGAAR+LKQ Sbjct: 65 SLAGADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQG 124 Query: 129 PQLPGSVHFVFQPGEEGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIM 188 P LPGSVHFVFQPGEEGGAGARKMIDDGLF QFPTEAVFGMHNWPGLPAGQFGLR GPIM Sbjct: 125 PMLPGSVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIM 184 Query: 189 AAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHA 248 AAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHA Sbjct: 185 AAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHA 244 Query: 249 GDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATIN 308 GDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMC ALAEAYGA+VEVEFFQYYPATIN Sbjct: 245 GDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATIN 304 Query: 309 TPAETDFCERVIRQTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSL 368 TPAETDFCERVIRQTFGD RIRTGIPANMTSEDFGFMLEERPG YVLIGNAP DR SHSL Sbjct: 305 TPAETDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSL 364 Query: 369 HHPHYDFNDDIIEEGVRYWVALAQSYFSSDL 399 HHPHYDFNDDIIE GVRYWVALAQ YF S L Sbjct: 365 HHPHYDFNDDIIEAGVRYWVALAQGYFGSGL 395 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 370 bits (949), Expect = e-103, Method: Compositional matrix adjust. Identities = 184/398 (46%), Positives = 242/398 (60%), Gaps = 8/398 (2%) Query: 6 MEIQHDAASVGSHRAHWASLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTG 65 M+I +D S+RR LHAHPELRFEE RTA V L++ G V +GG G Sbjct: 1 MDIVNDGIKELPKEMDLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYG 60 Query: 66 VVASLPGADPQRGIVLRADLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVL 125 VVA+L R I LRAD+DALP+ E N+F+H S G MHACGHDGHT MLLGAAR L Sbjct: 61 VVATLRKGKSTRAIALRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRL 120 Query: 126 KQMPQLPGSVHFVFQPGEEGGAGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTG 185 + + G+VHFVFQP EEGGAGAR MI+DGLF +FP +A+FG+HNWPGLPAG FGLR G Sbjct: 121 SREVEFDGTVHFVFQPAEEGGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPG 180 Query: 186 PIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCM 245 P+MA+ + T+ G+GAH AQPH +DP+ A + L Q+I R+ +P AVISV Sbjct: 181 PLMASSNTFSATLFGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQ 240 Query: 246 FHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPA 305 H G DNVIP++A L GT+R+ + ++ + + +A+ + + +V+ F + YPA Sbjct: 241 LHTGTADNVIPEQATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPA 300 Query: 306 TINTPAETDFCERVIRQTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGN------- 358 +N P T + + G ++ SEDF F L+ +PG Y +GN Sbjct: 301 VVNHPEATQQAMQAMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGK 360 Query: 359 -APADRPSHSLHHPHYDFNDDIIEEGVRYWVALAQSYF 395 A + S LH P YDFNDDII+ GV YWV+L + Y Sbjct: 361 QAGSQATSRELHSPFYDFNDDIIDAGVAYWVSLVRQYL 398 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 341 bits (874), Expect = 7e-95, Method: Compositional matrix adjust. Identities = 172/374 (45%), Positives = 240/374 (64%), Gaps = 5/374 (1%) Query: 24 SLRRDLHAHPELRFEEHRTAEVVVKELQALGYAVTRGLGGTGVVASLPGADPQRGIVLRA 83 ++RR LH HPEL FEE TA +V + L+ GYAVTR +GG G+VA+L R I LRA Sbjct: 24 AIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSARSIGLRA 83 Query: 84 DLDALPIHEANEFAHASCSHGIMHACGHDGHTVMLLGAARVLKQMPQLPGSVHFVFQPGE 143 D+DALPI E AS G MHACGHDGHT MLLGAAR L + + G+++ +FQP E Sbjct: 84 DMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIFQPAE 143 Query: 144 EGG--AGARKMIDDGLFTQFPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRLKITITGK 201 E G +GA+KM+ DGLF +FP EAVFG+HN PG+ AG F R+GP MAA +KI ITG+ Sbjct: 144 EAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRITGR 203 Query: 202 GAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAEL 261 G+HAA+PHL +DP+ +A S+V+ QT+ +R+ DP+D AV++V HAG NVIP+ A + Sbjct: 204 GSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVIPEFATM 263 Query: 262 RGTIRTLSSTLQQKLQEDIRRMCQALAEAYGAQVEVEFFQYYPATINTPAETDFCERVIR 321 ++R+ +++ L++ IR + A++YGAQ E+++ + YP +N+ AETDF V Sbjct: 264 ELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDFARSVAE 323 Query: 322 QTFGDARIRTGIPANMTSEDFGFMLEERPGTYVLIGNAPADRPSHSLHHPHYDFNDDIIE 381 + G ++ SEDF + L +RPG ++ +GN +P LH+ YDFND I Sbjct: 324 ELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQG-KP--MLHNAGYDFNDANIP 380 Query: 382 EGVRYWVALAQSYF 395 G YW L + + Sbjct: 381 IGAAYWTRLVERWM 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,419,743 Number of extensions: 64311 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 3 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)