BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0940.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 901 0.0 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 303 3e-83 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 297 1e-81 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 285 5e-78 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 279 3e-76 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 901 bits (2328), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/483 (90%), Positives = 460/483 (95%) Query: 6 SPLAHLTATGRLDHFYINGQWVPPQGNDRAAVVSPATEAAVCEIALGNAGDVDRAVQAAR 65 SPL HLT RLDHFYI+G+W PPQG++RAAVV+PATE VCEIALGNA DVD+AVQAAR Sbjct: 4 SPLTHLTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAAR 63 Query: 66 RALPDWAATSPWTRAAFLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR 125 RA+P WAATSP RAA LGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR Sbjct: 64 RAIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR 123 Query: 126 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS 185 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS Sbjct: 124 VARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPS 183 Query: 186 ELSPLSALLFAEIVHDAGIPAGVFNLVNGNGAEVGAAMSSHPQVDMVSITGSTRAGVLVA 245 ELSPLSALLFAEIVH+AG+PAGVFNLVNG+GAEVGAA+S+HPQVDM+SITGSTRAGVLVA Sbjct: 184 ELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVA 243 Query: 246 QAAATTVKRVAQELGGKSPNLVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAML 305 QAAA TVKRVAQELGGKSPNLVLPDADL RAIPPGVAAA RNMGQSCSAPTRLIVPRA L Sbjct: 244 QAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAAL 303 Query: 306 SQVHELALQTLAQMKVGDPSDAATTHGPLANRAQFLRVQQMIEVGMKDGARLLAGGPGRP 365 ++VHELAL TLAQMKVGDP+DAATTHGPLANRAQFLRVQQMIE G++DGARLLAGGPGRP Sbjct: 304 ARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRP 363 Query: 366 QGLEQGYYARPTIFSDVHTDMAIAQEEIFGPVLAILPYDSVEEAITIANDTVYGLGAHVQ 425 GL QGYYARPTIFS+VHTDM IAQEEIFGPVLAILPYD+VEEAI IANDTVYGLGAHVQ Sbjct: 364 DGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQ 423 Query: 426 GTDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKSILG 485 G DKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVK+ILG Sbjct: 424 GRDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAILG 483 Query: 486 YYA 488 YYA Sbjct: 484 YYA 486 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 303 bits (775), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 173/469 (36%), Positives = 263/469 (56%), Gaps = 10/469 (2%) Query: 21 YINGQWVPPQGNDRAAVVSPATEAAVCEIALGNAGDVDRAVQAARRALPDWAATSPWTRA 80 ++NGQW +R AV +PA + + L A + RA++AA A P W + R+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 81 AFLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR-VARDNLADY----P 135 A L R + L+L ++ A +T E G P++ AR + AA + A + Y P Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEAR-GEIGYAASFIEWFAEEGKRTYGDTIP 133 Query: 136 FIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLF 195 P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SAL Sbjct: 134 SPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALAL 193 Query: 196 AEIVHDAGIPAGVFNLVNGNGAEVGAAMSSHPQVDMVSITGSTRAGVLVAQAAATTVKRV 255 A + AGIPAGVF++V G +G M+S+P V +S TGST G L+ Q +A+++K++ Sbjct: 194 AVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKL 253 Query: 256 AQELGGKSPNLVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAMLSQVHELALQT 315 + ELGG +P +V DADL A+ +A+ +RN GQ+C R+ V + ++ Sbjct: 254 SLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEA 313 Query: 316 LAQMKVGDPSDAATTHGPLANRAQFLRVQQMIEVGMKDGARLLAGGPGRPQGLEQGYYAR 375 + + KVG ++ T GPL N +V+Q + + GAR+L G G+ L ++ Sbjct: 314 VKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLG--GKRHALGHSFF-E 370 Query: 376 PTIFSDVHTDMAIAQEEIFGPVLAILPYDSVEEAITIANDTVYGLGAHVQGTDKDQVRAV 435 PT+ +DV M +A+EE FGP+ + + + EE + +ANDT +GL ++ D ++ V Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 436 AARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYMEVKSI 483 A ++SG V +N + APFGG KQSG GRE G+++Y+ VK + Sbjct: 431 AEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYL 479 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 297 bits (761), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 184/486 (37%), Positives = 265/486 (54%), Gaps = 17/486 (3%) Query: 16 RLDHFYINGQWVPPQGNDRAAVVSPATEAAVCEIALGNAGDVDRAVQAARRALPDWAATS 75 R D+F I G++VPP + +SP A CE+A +A DV+ A+ AA A W TS Sbjct: 18 RYDNF-IGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTS 76 Query: 76 PWTRAAFLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR----VARDNL 131 P RA L ++ + EL A A TL+ G PI A +PLA +H R R Sbjct: 77 PTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 Query: 132 ADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLS 191 I EP+GV I PWN+PI K+ PALAAG VVLKP+E +P S Sbjct: 137 GSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPAS 196 Query: 192 ALLFAEIVHDAGIPAGVFNLVNGNGAEVGAAMSSHPQVDMVSITGSTRAGVLVAQAAATT 251 ++ E++ D IP GV N+V G G E G ++S+ ++ ++ TG T G L+ Q A+ Sbjct: 197 IMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQN 255 Query: 252 VKRVAQELGGKSPNLVLPDA-----DLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAMLS 306 + V ELGGKSPN+ D D G A N G+ C+ P+R++V ++ Sbjct: 256 LIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYE 315 Query: 307 QVHELALQTLAQMKVGDPSDAATTHGPLANRAQFLRVQQMIEVGMKDGARLLAGGPGRPQ 366 + E AL+ +A +K G+P D +T G A++ Q ++ I++G ++GA++LAGG GR + Sbjct: 316 RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-GREE 374 Query: 367 ---GLEQGYYARPTIFSDVHTDMAIAQEEIFGPVLAILPYDSVEEAITIANDTVYGLGAH 423 L GYY +PT+F + M I QEEIFGPV+++ + EEA+ IANDT+YGLGA Sbjct: 375 LGGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAG 433 Query: 424 VQGTDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKS 482 + D + + IQ+G+V N Y + A FGGYKQSG GRE ++ Y + K+ Sbjct: 434 LWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKN 493 Query: 483 ILGYYA 488 +L Y+ Sbjct: 494 LLVSYS 499 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 285 bits (730), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 189/478 (39%), Positives = 258/478 (53%), Gaps = 15/478 (3%) Query: 17 LDHFYINGQWVPPQGNDRAAVVSPATEAAVCEIALGNAGDVDRAVQAARRALPD--WAAT 74 L HF I G+ R +V+P E A G A DV RAV AA+ L W+ Sbjct: 5 LQHF-IAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKL 63 Query: 75 SPWTRAAFLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARD----- 129 S R L ++ AL+ ++L A +G R VP A HLR A Sbjct: 64 SGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQM 123 Query: 130 ---NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSE 186 + ++ + VREPIGV I PWN P+ + KV LAAGCTVV+KP+E Sbjct: 124 EGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAE 183 Query: 187 LSPLSALLFAEIVHDAGIPAGVFNLVNGNGAEVGAAMSSHPQVDMVSITGSTRAGVLVAQ 246 +P SAL A + +AG P GV N+V G G VG A+ HP V +S TGS AG + + Sbjct: 184 ETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQR 243 Query: 247 AAATTVKRVAQELGGKSPNLVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAMLS 306 A KRV ELGGKSP +V DA A+ F N GQ C+A +R++V R++ Sbjct: 244 IAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQ 303 Query: 307 QVHELALQTLAQMKVGDPSDAATTHGPLANRAQFLRVQQMIEVGMKDGARLLAGGPGRPQ 366 + + + A + VGDPS GP+A +AQF RV + I+ G+ +GA LLAGG RP Sbjct: 304 RFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRP- 362 Query: 367 GLEQGYYARPTIFSDVHTDMAIAQEEIFGPVLAILPYDSVEEAITIANDTVYGLGAHVQG 426 ++G++ +PTIF+D + M+IA+EEIFGPV I+ +D+ EEAI +AND+ YGL A V Sbjct: 363 --DKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 420 Query: 427 TDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKSI 483 T+ + VAA ++ G V +N + D P+GG K SG GRE G+ G Y E K++ Sbjct: 421 TNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 279 bits (714), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 163/472 (34%), Positives = 252/472 (53%), Gaps = 12/472 (2%) Query: 19 HFYINGQWVPPQGNDRAAVVSPATEAAVCEIALGNAGDVDRAVQAARRALPDWAATSPWT 78 +ING+W V++PATE + +IA + D+DRA++AA + W S + Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 79 RAAFLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLA------AEHLRVARDNLA 132 R+ + R LL ER+E A+ +T+E G P++ A+ + A AE R L Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 133 DYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 192 P P +++EP+G A TPWN+P+ Q+ K+ ALAAGC++++K E +P S Sbjct: 126 --PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 193 LLFAEIVHDAGIPAGVFNLVNGNGAEVGAAMSSHPQVDMVSITGSTRAGVLVAQAAATTV 252 DAG+PAGV NLV G AE+ + HP + +S TGST G +A A + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 253 KRVAQELGGKSPNLVLPDADLARAIPPGVAAAFRNMGQSCSAPTRLIVPRAMLSQVHELA 312 KR ELGG +P +V DAD+ A A FRN GQ C +PTR +V + + + + Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 313 LQTLAQMKVGDPSDAATTHGPLANRAQFLRVQQMIEVGMKDGARLLAGGPGRPQGLEQGY 372 + Q+KVG+ +A T GP+AN+ + ++ +I+ + GA+L GG + +GY Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGG---KRIGNKGY 360 Query: 373 YARPTIFSDVHTDMAIAQEEIFGPVLAILPYDSVEEAITIANDTVYGLGAHVQGTDKDQV 432 + +PT+ +++ EE FGP+ I +++++E IT AN YGL A+ Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 433 RAVAARIQSGQVHLNYPAWD-PQAPFGGYKQSGNGREYGVEGMEEYMEVKSI 483 A+AAR++SG + +N+ P+ PFGG K SG G E G E +E Y+ K + Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLV 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,560,393 Number of extensions: 67387 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 259 Number of HSP's successfully gapped: 21 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)