BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0927.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 875 0.0 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 539 e-154 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 398 e-112 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 317 1e-87 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 270 2e-73 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 875 bits (2262), Expect = 0.0, Method: Compositional matrix adjust. Identities = 462/493 (93%), Positives = 474/493 (96%) Query: 1 MNSLELQRPDLMPGTQLIGAQWRAAADGRVLEVSNPATGQVFATVPDGNAEDARAAVDAA 60 M SL LQR DLMPG QLIGA WR AADGR L+VS+PATGQVFA+VPDG A DARAAV+AA Sbjct: 1 MTSLTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAA 60 Query: 61 VAAFPAWRATPAKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVE 120 VAAF AWRATPAKQRA IIKRWNDLLLAHQ+DLGRLISREQGKPLAEAKGEVAYAASYVE Sbjct: 61 VAAFAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVE 120 Query: 121 WFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTV 180 WFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTV Sbjct: 121 WFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTV 180 Query: 181 VCKPAEDTPLTSLAIIRLALEAGVPAGVINIVTASRERTPEVVDVWLADSRVRKISFTGS 240 VCKPAEDTPLTSLA++RLA EAGVP GVINIVTASRERTPEVVDVWLAD RVRKISFTGS Sbjct: 181 VCKPAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGS 240 Query: 241 TAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADLDAAIDGVMAAKFRNGGQTCVSPNR 300 TAVGKHLARHSADTLKKLSLELGGNAPFIVFDDAD+DAAIDGVMAAKFRNGGQTCVSPNR Sbjct: 241 TAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNR 300 Query: 301 IYVQEKVYDAFVAKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIERGARV 360 IYVQEKVYDAFV KLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAI RGARV Sbjct: 301 IYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARV 360 Query: 361 ITGGKRLQGPGFDSDNYYAPTVLANVTGQMQCSCEETFGPVAPVTRFATEEEVVTAANAT 420 ITGGKRLQGPGF SDNYYAPTVLA+VTG MQCSCEETFGPVAP+TRFATE+EV+ AANAT Sbjct: 361 ITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANAT 420 Query: 421 PFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLD 480 PFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLD Sbjct: 421 PFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLD 480 Query: 481 DYLHTKYVCQGQL 493 DYLHTKYVCQGQL Sbjct: 481 DYLHTKYVCQGQL 493 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 539 bits (1389), Expect = e-154, Method: Compositional matrix adjust. Identities = 270/489 (55%), Positives = 351/489 (71%), Gaps = 9/489 (1%) Query: 4 LELQRPDLMPGTQLIGAQWRAAADGRVLEVSNPATGQVFATVPDGNAEDARAAVDAAVAA 63 L+L+ P L+ + QW A G L V NPA G++ VP A + R A++AA AA Sbjct: 2 LQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAA 61 Query: 64 FPAWRATPAKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFG 123 +PAW+ AK+R+ I++RW +L+LA+ +DL +++ EQGKPLAEA+GE+ YAAS++EWF Sbjct: 62 WPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFA 121 Query: 124 EEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCK 183 EE R GD IP+P R++ +KE +GV AAITPWNFPAAMI RK PALAAGC +V K Sbjct: 122 EEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLK 181 Query: 184 PAEDTPLTSLAIIRLALEAGVPAGVINIVTASRERTPEVVDVWL-ADSRVRKISFTGSTA 242 PAE TP ++LA+ LA AG+PAGV ++VT TP+ + + ++ VRKISFTGST Sbjct: 182 PAEATPFSALALAVLAERAGIPAGVFSVVTG----TPKGIGGEMTSNPIVRKISFTGSTG 237 Query: 243 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADLDAAIDGVMAAKFRNGGQTCVSPNRIY 302 VGK L + SA ++KKLSLELGGNAPFIVFDDADLDAA++G +A+K+RN GQTCV NRIY Sbjct: 238 VGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIY 297 Query: 303 VQEKVYDAFVAKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIERGARVIT 362 VQ+ VYDAF AKL V KVG T+ GP+IN +A+ K++QHV DA+ +GARV+ Sbjct: 298 VQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLL 357 Query: 363 GGKRLQGPGFDSDNYYAPTVLANVTGQMQCSCEETFGPVAPVTRFATEEEVVTAANATPF 422 GGKR +++ PTVLA+VT MQ + EETFGP+AP+ RF T+EEV+ AN T F Sbjct: 358 GGKRHA----LGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEF 413 Query: 423 GLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDY 482 GLA+YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG GREGS +G+DDY Sbjct: 414 GLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDY 473 Query: 483 LHTKYVCQG 491 L KY+C G Sbjct: 474 LVVKYLCMG 482 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 398 bits (1022), Expect = e-112, Method: Compositional matrix adjust. Identities = 207/474 (43%), Positives = 291/474 (61%), Gaps = 7/474 (1%) Query: 17 LIGAQWRAAADGRVLEVSNPATGQVFATVPDGNAEDARAAVDAAVAAFPAWRATPAKQRA 76 I +W A+A GR ++V NPAT +V + + D A++AA F WR A +R+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 77 TIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPA 136 I++R DLL E++ RL++ EQGKPLAEAK E AA ++WF EEA R G ++PA Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 137 PVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLAII 196 V G M +KEPVG VAA TPWNFP + RK++ ALAAGC+++ K E+TP + +I Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 197 RLALEAGVPAGVINIVTASRERTPEVVDVWLADSRVRKISFTGSTAVGKHLARHSADTLK 256 R +AGVPAGVIN+V E+ + + ++KISFTGST VGK LA + +K Sbjct: 188 RAYADAGVPAGVINLVYGV---PAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 257 KLSLELGGNAPFIVFDDADLDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVAKLG 316 + ++ELGG+AP +VFDDAD+D A + AKFRN GQ CVSP R VQ+ V++AFV K Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 317 ARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIERGARVITGGKRLQGPGFDSDN 376 LKVG + +GP+ N R I ++ + DA+ +GA++ TGGKR+ G+ Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY---- 360 Query: 377 YYAPTVLANVTGQMQCSCEETFGPVAPVTRFATEEEVVTAANATPFGLAAYFYSTDVRRI 436 ++ PTVL N+ Q + EE FGP+A + F T +EV+T AN P+GLAAY ++ Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 437 HRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVCQ 490 H + +ESG++ +N LA PFGG+K+SGYG EG ++ Y++TK V Q Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQ 474 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 317 bits (812), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 189/481 (39%), Positives = 277/481 (57%), Gaps = 8/481 (1%) Query: 11 LMPGTQL-IGAQWRAAADGRVLEVSNPATGQVFATVPDGNAEDARAAVDAAVAAFPAWRA 69 + P QL I ++ R EV +PATG V +P D A+ AA AF WR Sbjct: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 Query: 70 TPAKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRA 129 +R+ I++R L + + I+ + GKPLA+A E+ AA +++W EE R Sbjct: 61 VSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 Query: 130 NGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTP 189 G ++ A R + ++EPVGV AAI+PWNFP +K+A A+AAGCTVV K ++P Sbjct: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 Query: 190 LTSLAIIRLALEAGVPAGVINIVTASRERTPEVVDVWLADSRVRKISFTGSTAVGKHLAR 249 + + +L +AGVPAGV NIV+ + E+ + VR ISFTGS VG LA Sbjct: 181 AAIVLMAQLFHDAGVPAGVFNIVSGV---SSEISQHLIESPLVRNISFTGSVPVGSKLAA 237 Query: 250 HSADTLKKLSLELGGNAPFIVFDDADLDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYD 309 +A +K++S+ELGG+AP +VFDDA+++ A + K RN GQ CVSP+R YVQ+ +Y Sbjct: 238 QAALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYA 297 Query: 310 AFVAKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIERGARVITGGKRLQG 369 FV + +KVGP +P Q+GP+I+ R + + DA+ +G+ ++TGG RL Sbjct: 298 RFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQ 357 Query: 370 PGFDSDNYYAPTVLANVTGQMQCSCEETFGPVAPVTRFATEEEVVTAANATPFGLAAYFY 429 G ++YAPT++ V + EE FGP+A VT F+ EE + ANA P GLA+Y + Sbjct: 358 RG----HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVF 413 Query: 430 STDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVC 489 + + HRV+ L++G+V +N +A PFGG+ ESG+G EG V G + +L TK + Sbjct: 414 TQSLDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 Query: 490 Q 490 Q Sbjct: 474 Q 474 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 270 bits (689), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 174/491 (35%), Positives = 255/491 (51%), Gaps = 15/491 (3%) Query: 3 SLELQRPDLMPGTQLIGAQW----RAAADGRVLEVSNPATGQVFATVPDGNAEDARAAVD 58 +L L PG + IG+ W A G ++++ NPATG+ + DG A AA Sbjct: 8 NLMLAFGHFFPGAKEIGS-WINGELVAGQGEIIQLYNPATGRASLSYRDGGAAAVEAAAV 66 Query: 59 AAVAAFPAWRATPAKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASY 118 AA A W A R + ++ A E L RL + GKP+ + + E+ A Sbjct: 67 AAQRAQRQWWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEM 126 Query: 119 VEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGC 178 E++ A + G++IP P + +EP G V +TPWN PA ++ PALA G Sbjct: 127 FEYYAGWADKFYGEVIPVP-SSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGN 185 Query: 179 TVVCKPAEDTPLTSLAIIRLALEAGVPAGVINIVTA-SRERTPEVVDVWLADSRVRKISF 237 V+ KP+E TP +SLAI RL +AG+PAG++N++ + P+ + W V+K+ F Sbjct: 186 AVLLKPSELTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWT----VKKVIF 241 Query: 238 TGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADLDAAIDGVMAAKFRNGGQTCVS 297 GS A G +A+ +A + LELGG + I+F+DADL A G AA F GQ+CV+ Sbjct: 242 VGSPATGALIAKAAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVA 301 Query: 298 PNRIYVQEKVYDAFVAKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIERG 357 +R+ VQ KVYD FV + A +++G D ++++GP+ N + I + V +E G Sbjct: 302 GSRLLVQRKVYDRFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAG 361 Query: 358 ARVITGGKRLQGPGFDSDNYYAPTVLANVTGQMQCSCEETFGPVAPVTRFATEEEVVTAA 417 A + G R G+ + PT+LA+ + M+ + E FGPVA F EEE + A Sbjct: 362 ATLAAGHTRYGEEGY----FVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIA 417 Query: 418 NATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVH 477 N + FGLA ++ DV R HRV ++ +G VN A+PFGG SGYGR + Sbjct: 418 NDSEFGLAGAVWTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGME 477 Query: 478 GLDDYLHTKYV 488 L DY TK V Sbjct: 478 ALYDYTQTKSV 488 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,540,575 Number of extensions: 65225 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 21 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)