BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0890.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 672 0.0 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 162 5e-41 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 85 7e-18 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 80 2e-16 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 72 9e-14 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 672 bits (1734), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/345 (94%), Positives = 339/345 (98%) Query: 1 MQQGLVIHAPHDLRIEPLDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 MQQGLVIHAPHDLRI+ LDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV Sbjct: 1 MQQGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 Query: 61 LGHEVSGQIAEVGSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCLDMRFYGSAMRTP 120 LGHEVSGQIAEVG+A++DIAPGTRIAISPSRPCG CKYCQEGKQNHCLDMRFYGSAMRTP Sbjct: 61 LGHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTP 120 Query: 121 HVQGAFRQEIIIERSQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGP 180 HVQGAFRQEIIIERSQAH+VADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGP Sbjct: 121 HVQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGP 180 Query: 181 IGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMAETPDALKAYAADKGTFDV 240 IGALVVVAARRAGALEVVVTDVA MPL+ AL+VGAD AINMAETP ALKAYAADKGTFDV Sbjct: 181 IGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDV 240 Query: 241 LFEASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELDWRGAFRFHEEFAMA 300 LFEASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKEL+WRGAFRFHEEFAMA Sbjct: 241 LFEASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMA 300 Query: 301 VELMNRKLVDVKPLISATYPISQAVDAFEIAGDRSQMMKVQISFD 345 VELMNRKLVDVKPLI+ATYPI+QAV+AFEIAGDRS+MMKVQISFD Sbjct: 301 VELMNRKLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISFD 345 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 162 bits (409), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 117/351 (33%), Positives = 176/351 (50%), Gaps = 16/351 (4%) Query: 3 QGLVIHAPHDLRIEPLDTGALQPQQL-----KVKVKAGGICGSDLHYYHHGGFGVVRIKE 57 Q LV+ A +L++ +D PQQ+ ++++ GICGSDLHYY HG G +++ Sbjct: 2 QALVLEATRELKLREIDL----PQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEA 57 Query: 58 PMVLGHEVSGQIAEVGSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCLDMRFYGSAM 117 PMVLGHE SG + EVGSA+S + G R+ + P P G N +RF+ Sbjct: 58 PMVLGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWA--- 114 Query: 118 RTPHVQGAFRQEIIIERSQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTG 177 TP + G ++ + + + D+VS E A+ EPLS+ L A +A G +V G Sbjct: 115 -TPPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIG 173 Query: 178 CGPIGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMA-ETPDALKAYAADKG 236 G IGA+ +AA GA V++ DV L A +++ ET + A D Sbjct: 174 AGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGW 233 Query: 237 TFDVLFEASGNERALVGALEALRPQAIIVQVGL-GGDIQFPANLLVGKELDWRGAFRFHE 295 DV+FEASG+ L+ + P V VG+ + + KE+ FR+ Sbjct: 234 GADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYAN 293 Query: 296 EFAMAVELMNRKLVDVKPLISATYPISQAVDAF-EIAGDRSQMMKVQISFD 345 F A+ L++ ++DVKP IS +P SQ++ AF E A R Q +K+QI + Sbjct: 294 IFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEME 344 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 85.1 bits (209), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 81/276 (29%), Positives = 117/276 (42%), Gaps = 24/276 (8%) Query: 13 LRIEPLDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 L+IE LD P ++ V++KA G+C SDL GV PM LGHE S ++ EV Sbjct: 23 LKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVI----TGVRPRPVPMALGHEASAEVVEV 78 Query: 73 GSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCL--------------DMRFYGSAMR 118 GS ++D+ G + + CG C C EG+ C R Sbjct: 79 GSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDKTQP 138 Query: 119 TPHVQG--AFRQEIIIERSQAHIVADHVSDGEAAM--AEPLSVALHAVRRAGPLVGKRVL 174 H G AF ++ R + + +AA+ L+ AV A G Sbjct: 139 VHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGTTAA 198 Query: 175 VTGCGPIGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMAETPDAL-KAYAA 233 V G G +G ++ A +GA EV+ D+ L A +GA +N A PDA+ K A Sbjct: 199 VLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVN-ARDPDAVEKVRAL 257 Query: 234 DKGTFDVLFEASGNERALVGALEALRPQAIIVQVGL 269 +G D FE +G+ +A+ A R + V GL Sbjct: 258 TRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGL 293 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 76/282 (26%), Positives = 126/282 (44%), Gaps = 12/282 (4%) Query: 13 LRIEPLDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 L IE + P Q+ VK +A G+C +DLH H G V+ P + GHE +G +A V Sbjct: 17 LSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAH--GDWPVKPTPPFIPGHEGTGYVAAV 74 Query: 73 GSAISDIAPGTRIAISP-SRPCGLCKYCQEGKQNHCLDMRFYGSAMRTPHVQGAFRQEII 131 G+ + + G R+ + CG C C+ G + C + + G + V G+F + + Sbjct: 75 GAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYS-----VNGSFAEYGL 129 Query: 132 IERSQAHIVADHVSDGEAAMAEPLSVALH-AVRRAGPLVGKRVLVTGCGPIGALVVVAAR 190 + + D++ G AA V ++ ++ G+ V+++G G +G + V A+ Sbjct: 130 ADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMAVQYAK 189 Query: 191 RAGALEVVVTDVARMPLETALKVGADRAINMAETPDALKAYAADKGTFDVLFEASGNERA 250 G + VV D+ L A K+GAD ++ + G L A + +A Sbjct: 190 AMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHGALVTAV-SPKA 247 Query: 251 LVGALEALRPQAIIVQVGL-GGDIQFPANLLVGKELDWRGAF 291 + A LR + + VGL GDI P V K + RG+ Sbjct: 248 MEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSI 289 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 71.6 bits (174), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 87/317 (27%), Positives = 125/317 (39%), Gaps = 56/317 (17%) Query: 3 QGLVIHAPHDLRIEPLDTGALQ-PQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 + L+ H+ HD+R+E + +Q + +KV A ICGSDLH Y + K +L Sbjct: 2 RALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGK---IPATKAGDIL 58 Query: 62 GHEVSGQIAEVGSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCLDMRFYGSAMRTPH 121 GHE G + E G AI + G R+ + CG C +C D Y + T Sbjct: 59 GHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFC---------DQHLYAACETTNP 109 Query: 122 VQGAFRQEIIIERSQAHIVADHVSDG---------------------EAAMAEP------ 154 +GA I A H+ G ++A+A+ Sbjct: 110 DRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLS 169 Query: 155 --LSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALEVVVTDVARMPLETA-- 210 L AV+ AG G V + G GP+G + +AR GA + + D L A Sbjct: 170 DILPTGYQAVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQE 229 Query: 211 ----LKVGADRAINMAETPDALKAYAADKGTFDVL-FEASGN--ERALVG-ALEALRPQA 262 + V D+ ++ AE A A G D + FEA G+ E A+ LE QA Sbjct: 230 QYDVIPVNFDQ-VDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQA 288 Query: 263 I---IVQVGLGGDIQFP 276 + I V GG I P Sbjct: 289 LRHCIASVRRGGVISVP 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,036,361 Number of extensions: 42068 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 7 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)