BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0889.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 522 e-149 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 144 7e-36 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 143 1e-35 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 142 2e-35 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 141 6e-35 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 522 bits (1344), Expect = e-149, Method: Compositional matrix adjust. Identities = 255/264 (96%), Positives = 260/264 (98%) Query: 1 MTIKTTPLPAGLQGFSLEGRLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAKVA 60 MT KTTPLPAGLQGFSLEG+LALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLA VA Sbjct: 1 MTTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA 60 Query: 61 AQLRAEGHTIHESSFDATSAEDVKAAVQKIESEIGAIDILVNNAGIQRRNPLEEFKESDW 120 AQLRAEGHTIHESSFDATS+E+VKAAVQKIESEIG I ILVNNAGIQRRNPLEEFKESDW Sbjct: 61 AQLRAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDW 120 Query: 121 HDLINTNLNSVFYAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 HDLI+TNLNSVF+AAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK Sbjct: 121 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 Query: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 240 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF Sbjct: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVF 240 Query: 241 LASDASRFVNGHILYVDGGVTATL 264 LASDASRFVNGHILYVDGGVTATL Sbjct: 241 LASDASRFVNGHILYVDGGVTATL 264 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 144 bits (363), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 82/245 (33%), Positives = 132/245 (53%), Gaps = 3/245 (1%) Query: 17 LEGRLALITGSSGGIGLALARGMAQAGAAIVLN-GRDAGKLAKVAAQLRAEGHTIHESSF 75 + R+A +TG GGIG A+ + + G +V G ++ + K A +R +G IH S Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 76 DATSAEDVKAAVQKIESEIGAIDILVNNAGIQRRNPLEEFKESDWHDLINTNLNSVFYAA 135 + ++ E KAA +K+ +EIG +D+LVNNAGI R + + DW +I+TNLNS+F Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 136 QAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNG 195 + V M RG G+IINI SV + G+ G Y+ K + FT +A + G+ VN Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 196 LGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILY 255 + PGY T+M A+ + + +V P +R G+ E++ ++AS + G Sbjct: 181 VSPGYIGTDMVRAI--RPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFS 238 Query: 256 VDGGV 260 ++GG+ Sbjct: 239 LNGGL 243 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 143 bits (361), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 94/249 (37%), Positives = 128/249 (51%), Gaps = 6/249 (2%) Query: 16 SLEGRLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAKVAAQLRAEGHTIHESSF 75 +L+G++ALITG+S GIG A A A+ GAA++L R L +VAA + A+G + Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAG 62 Query: 76 DATSAEDVKAAVQKIESEIGAIDILVNNAG-IQRRNPLEEFKESDWHDLINTNLNSVFYA 134 D A + V E G +DI +NNAG + PL E W D ++ NL S F Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 135 AQAVARHMIPRGAGKIINICS-VQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQV 193 A+ M+ RGAG I+ S V S G PG+A Y A K + KG+A D+ GI+ Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 194 NGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHI 253 N L PG T+M D + W G +R E++ AA+FLAS + FV G Sbjct: 183 NALLPGGVDTDMG----GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTA 238 Query: 254 LYVDGGVTA 262 LY DGG +A Sbjct: 239 LYADGGNSA 247 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 142 bits (359), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 88/255 (34%), Positives = 132/255 (51%), Gaps = 5/255 (1%) Query: 9 PAGLQGFSLEGRLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAKVAAQLRAEGH 68 P+ LQ F EG++ ++TG++ GIG A AR + GA ++L RDA L KV L E Sbjct: 6 PSRLQRF--EGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERT 63 Query: 69 TIHESSFDATSAEDVKAAVQKIESEIGAIDILVNNAGIQRRNPLEEFKESDWHDLINTNL 128 E+ D + E V+ V G +D+LV++AG+ + E DWH + TN+ Sbjct: 64 AARET--DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNV 121 Query: 129 NSVFYAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGK 188 N VFY A+ H+ + G I+N+ SV ++ Y A+KGAV T+ MA+D+G+ Sbjct: 122 NGVFYGAREALPHL-EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 Query: 189 YGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRF 248 G+++N + P T M + +D D R R D ++ FLAS + F Sbjct: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 Query: 249 VNGHILYVDGGVTAT 263 VNG L VDGG+ A+ Sbjct: 241 VNGVNLPVDGGLMAS 255 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 141 bits (355), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 80/243 (32%), Positives = 127/243 (52%), Gaps = 1/243 (0%) Query: 20 RLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAKVAAQLRAEGHTIHESSFDATS 79 R+AL+TG + GIG ++++ +A G +++ + +K A L A GH D S Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 80 AEDVKAAVQKIESEIGAIDILVNNAGIQRRNPLEEFKESDWHDLINTNLNSVFYAAQAVA 139 E + AA IE + G D+LVNNAG+ R ++ +W +N N+ V A Q A Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 140 RHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPG 199 R M+ + G+I+NI S+ G Y +K AV T+ MAI+ +YGI VN + PG Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 200 YFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGG 259 T M A+ ++ + P RR+G +++ A +LA++ S ++ GH++ VDGG Sbjct: 189 PVDTPMTQAMHSEQTRESYY-RLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 260 VTA 262 A Sbjct: 248 FVA 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 784,834 Number of extensions: 30861 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 49 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)