BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0881.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 847 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 100 2e-22 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 95 9e-21 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 76 6e-15 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 847 bits (2187), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/440 (93%), Positives = 428/440 (97%) Query: 1 LENILLIVAAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGLRGRILAVVHNQLDAYL 60 +ENILLIV AFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQG+RGRILAVVHNQLDAYL Sbjct: 1 MENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 Query: 61 SACQLGITLASLGLGWIGEPAFARLLEPIFTLLGVSNQELIHGISFVFAFLVISFLHIVA 120 SACQLGITLASLGLGWIGEPAFAR+LEP+F+L GV+NQELIHG+SFVFAF VISFLHIVA Sbjct: 61 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA 120 Query: 121 GELAPKSMAIRSPEKLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAAHGHDAH 180 GELAPKSMAIRSPEKLGLWCA+PLYGFYWGMYPLIWVLNASSNWLLRVAGLGA HGHDAH Sbjct: 121 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 Query: 181 YSSDELKLILRAGGKSGKSGKFTRDEWNVLVQSLNFAELDVADIMRPASEIVALGDDKSL 240 YSSDELKLILRAG KSGK+GKFTRDEWNVL QSLNFAELDVADIMRPASEIVALGDDKSL Sbjct: 181 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL 240 Query: 241 EENLDIIYRNRYSRYPYFDAERQQVLGLVHLKDVFLAQQDGRAISNLKDYLRPVQFISPA 300 EENLDIIYRNRYSRYPY+DAERQQVLGLVHLKDVFLAQQDGRAI+NLKDYLRPVQ+ISPA Sbjct: 241 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA 300 Query: 301 LPALDLLRRFRTGMPHFAIIGKKGQSPLGFITLDNMLSLLVGEIRDEFRHNTGEWSRQDD 360 LPALDLLRRFRTG PHFA+IGKKGQ P GFITLDNMLSLLVGEIRDEFRHNTGEW+RQDD Sbjct: 301 LPALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD 360 Query: 361 GTLLGKGSLPIVTLENMLGIDIDYDDSIDSVGGLVMEKLGDLPKEGQKIEFAAFDVVIKR 420 GTLLGKGSLPIVTLENMLGIDI+YDDSIDSVGGLVMEKLGDLPKEGQKI+F AFDVVIKR Sbjct: 361 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 421 MSGPKIVLVKVYPKVGEVRE 440 MSGPKIVLVKVYPK+ E RE Sbjct: 421 MSGPKIVLVKVYPKLQEARE 440 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 95/435 (21%), Positives = 193/435 (44%), Gaps = 35/435 (8%) Query: 7 IVAAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGLRGRILAVVHNQLDAYLSACQLG 66 ++A F L+ L+ FF E +L+ + R+R +AK QG R I + L Sbjct: 10 VLALFILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAITIL-----------WLLD 57 Query: 67 ITLASLGLGWIGEPAFARLLEPIFTLLGVS----NQELIHGISFVFAFLVISFLHIVAGE 122 T L L I A L + T L ++ +Q +I + A L+I F E Sbjct: 58 RTERLLSLVLIANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIF-----AE 112 Query: 123 LAPKSMAIRSPEKLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGA-AHGHDAHY 181 + PK + PE++ L+ L PLIW +N + +LR+ + A + Sbjct: 113 IVPKVIGATYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRI 172 Query: 182 SSDELKLILRAGGKSGKSGKFTRDEWNVLVQSLNFAELDVADIMRPASEIVALGDDKSLE 241 S +EL+ I+ GG + ++L+ + ++ V D+M P S++ AL ++ Sbjct: 173 SPEELRSIVLEGGSF-----IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVD 227 Query: 242 ENLDIIYRNRYSRYPYFDAERQQVLGLVHLKDV--FLAQQDGRAISNLKDYLRPVQFISP 299 + L + +++ P F+ E ++G++H++ +++ + ++++ L ++ Sbjct: 228 DILHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPV 287 Query: 300 ALPALDLLRRFRTGMPHFAIIGKKGQSPLGFITLDNMLSLLVGEIRDEFRHNTGE---WS 356 L+ F+ ++ + G +TL++++ ++GE ++GE W Sbjct: 288 ETDVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWD 347 Query: 357 RQDDGTLLGKGSLPIVTLENMLGIDIDYDDSIDSVGGLVMEKLGDLPKEGQKIEFAAFDV 416 + DG L +GS + + LG+ + D ++ GL++E L D+P+ ++ + Sbjct: 348 K--DGACLLEGSTTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCVI 404 Query: 417 VIKRMSGPKIVLVKV 431 I ++ I +VK+ Sbjct: 405 EIVQVQNQAIRMVKL 419 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 95.1 bits (235), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 94/415 (22%), Positives = 179/415 (43%), Gaps = 16/415 (3%) Query: 17 NGFFVAAEFSLVKLRQTRIRAIAKTQGLRG--RILAVVHNQLDAYLSACQLGITLASLGL 74 + F AE S+ R+ R+ ++K +G+ R+L++ Q + + Q+G+ ++ Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSK-EGVHNADRVLSL-QAQPGNFFTVVQIGLNAVAILG 74 Query: 75 GWIGEPAFARLLEPIFTLLGVSNQELIHGISFVFAFLVISFLHIVAGELAPKSMAIRSPE 134 G +GEPA + I + + + SF +FLV++ I+ +L PK +A+ +PE Sbjct: 75 GVVGEPALTPHMREIISSFYIG--PWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 135 KLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAAHGHDAHYSSDELKLILRAGG 194 + + P+ PL+W N+ S W+ + GL + +E+ ++ AG Sbjct: 133 RAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQE--VITPNEIHAMVDAG- 189 Query: 195 KSGKSGKFTRDEWNVLVQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSR 254 ++G E +++ V M P IV S I + +SR Sbjct: 190 --AQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSR 247 Query: 255 YPYFDAERQQVLGLVHLKDVFLAQQDGRAIS-NLKDYLRPVQFISPALPALDLLRRFRTG 313 + D VLG + KDV G S K + P I +L ++L R + Sbjct: 248 FLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAM 307 Query: 314 MPHFAIIGKKGQSPLGFITLDNMLSLLVGEIRDEFRHNTGEWSRQDDGTLLGKGSLPIVT 373 AI+ + +G I+ ++ +L+G + + R+D+ + L G P+ Sbjct: 308 DEDLAIVVNEYALVVGIISTTDVTGVLMGNLL-AITEEEQQIVRRDENSWLMDGLTPLND 366 Query: 374 LENMLGIDIDYDDSI--DSVGGLVMEKLGDLPKEGQKIEFAAFDVVIKRMSGPKI 426 + + L ID +Y + + +++ G +M L +PK+ + FA F + + G +I Sbjct: 367 VMSELDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 75.9 bits (185), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 61/226 (26%), Positives = 112/226 (49%), Gaps = 13/226 (5%) Query: 217 AELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYFDAERQQVLGLVHLKDV-- 274 ++L V DIM P S++ + K +E + + +SR+P D ER +V+G++ KD+ Sbjct: 64 SDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVGILLAKDLLR 123 Query: 275 FLAQQDGRAISNLKDYLRPVQFISPALPALDLLRRFRTGMPHFAIIGKKGQSPLGFITLD 334 + A++ +++D LRP FI + LLR FR H A++ + G IT++ Sbjct: 124 YYAEES----FDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGVAGLITIE 179 Query: 335 NMLSLLVGEIRDEFRHNTGEWS--RQDDGTLLGKGSLPIVTLENMLGIDID---YDDSID 389 ++L +VG+I DE+ + E + DG + + +T ++ DD D Sbjct: 180 DVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNEEVGSRFSDDDAD 239 Query: 390 SVGGLVMEKLGDLPKEGQKIEFAA--FDVVIKRMSGPKIVLVKVYP 433 ++GGLV +G +P++G + + F+V+ ++LV P Sbjct: 240 TIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,298,691 Number of extensions: 54579 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 4 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)