BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0872.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 943 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 491 e-140 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 377 e-105 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 336 2e-93 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 304 1e-83 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 943 bits (2438), Expect = 0.0, Method: Compositional matrix adjust. Identities = 457/484 (94%), Positives = 470/484 (97%) Query: 2 LHQLKDPSLLRQQAYLNGAWCDADSGAKLEVHNPANGQLLGHVPLMGATETRRAIDAANA 61 + QLKDP+LLRQQA++NG WCDAD G +L VHNPANG+LLGHVPLMGA ETRRAI+AANA Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 62 AWPAWKKKTAKERSALLRRWYELMLANADDLALIMTAEQGKPLAEARGEIGYAASFIEWF 121 AWPAWKKKTAKERSA+LRRWYELMLAN DDLALIMTAEQGKPLAEARGEIGYAASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 122 AEEGKRTYGDTIPSPSPTNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 181 AEEGKRTYGDTIPSPSP+NRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 182 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKEIGGEMTSNPIVRKISFTGSTGVGK 241 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPK IGGEMTSNPIVRKISFTGSTGVGK Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 242 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 301 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 Query: 302 GVYDAFAAKLVEAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHVADALAKGARVLLGGK 361 GVYDAFAAKLVEAVKK KVG G E GVTQGPLINEQAV+KVEQHVADA+AKGARVLLGGK Sbjct: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 Query: 362 RHALGHGFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDDEALSLANDTEFGLASYFY 421 RHALGH FFEPTVLADVTPAMQVAREETFGPMAPLFRFKTD+E L+LANDTEFGLASYFY Sbjct: 361 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFY 420 Query: 422 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 481 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC Sbjct: 421 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 Query: 482 MGGI 485 MGGI Sbjct: 481 MGGI 484 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 491 bits (1263), Expect = e-140, Method: Compositional matrix adjust. Identities = 270/486 (55%), Positives = 352/486 (72%), Gaps = 13/486 (2%) Query: 11 LRQQAYLNGA------WCDADSGAKLEVHNPANGQLLGHVPLMGATETRRAIDAANAAWP 64 L++Q + GA W A G +L+V +PA GQ+ VP GA + R A++AA AA+ Sbjct: 6 LQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFA 65 Query: 65 AWKKKTAKERSALLRRWYELMLANADDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEE 124 AW+ AK+R+ +++RW +L+LA+ DDL +++ EQGKPLAEA+GE+ YAAS++EWF EE Sbjct: 66 AWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEE 125 Query: 125 GKRTYGDTIPSPSPTNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPA 184 R GD IP+P R++ +KE +GV AAITPWNFPAAMI RK PALAAGC +V KPA Sbjct: 126 ATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPA 185 Query: 185 EATPFSALALAVLAERAGIPAGVFSVVTGTPK---EIGGEMTSNPIVRKISFTGSTGVGK 241 E TP ++LAL LA+ AG+P GV ++VT + + E+ ++ VRKISFTGST VGK Sbjct: 186 EDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGK 245 Query: 242 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 301 L + SA ++KKLSLELGGNAPFIVFDDAD+DAA++G +A+K+RN GQTCV NRIYVQ+ Sbjct: 246 HLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQE 305 Query: 302 GVYDAFAAKLVEAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHVADALAKGARVLLGGK 361 VYDAF KL V LKVG + GP+IN +A+ K++QHV DA+A+GARV+ GGK Sbjct: 306 KVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGK 365 Query: 362 R-HALGHG---FFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDDEALSLANDTEFGLA 417 R G G ++ PTVLADVT AMQ + EETFGP+AP+ RF T+DE ++ AN T FGLA Sbjct: 366 RLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLA 425 Query: 418 SYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVV 477 +YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG GREGS +G+DDYL Sbjct: 426 AYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHT 485 Query: 478 KYLCMG 483 KY+C G Sbjct: 486 KYVCQG 491 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 377 bits (968), Expect = e-105, Method: Compositional matrix adjust. Identities = 199/469 (42%), Positives = 293/469 (62%), Gaps = 2/469 (0%) Query: 14 QAYLNGAWCDADSGAKLEVHNPANGQLLGHVPLMGATETRRAIDAANAAWPAWKKKTAKE 73 Q ++NG W + SG ++V NPA +++G + + RA++AA+ + W+ +A E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 74 RSALLRRWYELMLANADDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 133 RS ++RR +L+ A+++A +MT EQGKPLAEA+ E AA I+WFAEE +RTYG + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 134 PSPSPTNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 193 P+ P +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 194 LAVLAERAGIPAGVFSVVTGTPKEIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 253 L AG+PAGV ++V G P EI + +P+++KISFTGST VGK L + +K+ Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 254 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 313 ++ELGG+AP +VFDDAD+D A + +K+RNAGQ CV R VQ GV++AF K V Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 Query: 314 AVKKLKVGDGMENGVTQGPLINEQAVKKVEQHVADALAKGARVLLGGKRHALGHGFFEPT 373 ++LKVG+G+E G+T GP+ N++ + +E + DA+++GA++ GGKR FF+PT Sbjct: 306 YAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPT 365 Query: 374 VLADVTPAMQVAREETFGPMAPLFRFKTDDEALSLANDTEFGLASYFYSRDIGRIWRVAE 433 VL ++ + EE FGP+A + F+T DE ++ AN +GLA+Y +++ +A Sbjct: 366 VLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAA 425 Query: 434 GLESGMVGIN-TGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 481 +ESGM+ IN GL E+ PFGG+K SG G EG I+ Y+ K + Sbjct: 426 RVESGMLTINHLGLALPEL-PFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 336 bits (862), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 191/468 (40%), Positives = 284/468 (60%) Query: 14 QAYLNGAWCDADSGAKLEVHNPANGQLLGHVPLMGATETRRAIDAANAAWPAWKKKTAKE 73 Q Y++G + + EV +PA G +LG +P + AI AA+ A+ W++ + E Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 74 RSALLRRWYELMLANADDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 133 RS +LRR L A +A +T + GKPLA+A EI AA ++W AEEG+R YG + Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 134 PSPSPTNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 193 + P R +V++E +GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 Query: 194 LAVLAERAGIPAGVFSVVTGTPKEIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 253 +A L AG+PAGVF++V+G EI + +P+VR ISFTGS VG L Q+A +K+ Sbjct: 186 MAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKR 245 Query: 254 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 313 +S+ELGG+AP +VFDDA+++ A K RNAGQ CV +R YVQ G+Y F E Sbjct: 246 MSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAE 305 Query: 314 AVKKLKVGDGMENGVTQGPLINEQAVKKVEQHVADALAKGARVLLGGKRHALGHGFFEPT 373 A+ ++KVG G E GV GPLI+ + + V+ + DALA+G+ ++ GG R F+ PT Sbjct: 306 ALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAPT 365 Query: 374 VLADVTPAMQVAREETFGPMAPLFRFKTDDEALSLANDTEFGLASYFYSRDIGRIWRVAE 433 ++ V P+ +V +EE FGP+A + F +EA+++AN GLASY +++ + RV+ Sbjct: 366 LITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVSR 425 Query: 434 GLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 481 L++GMV IN +++ PFGG+ +SG G EG G + +LV K + Sbjct: 426 DLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 304 bits (779), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 176/469 (37%), Positives = 263/469 (56%), Gaps = 14/469 (2%) Query: 16 YLNGAWCDADSGAKLEVHNPANGQLLGHVPLMGATETRRAIDAANAAWPAWKKKTAKERS 75 Y++G W + V PA + + + L A + +A+ AA A PAW + ER+ Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 76 ALLRRWYELMLANADDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI-- 133 ALL R + L+L ++ A +T E G P I YA + A E R D + Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLAD 131 Query: 134 -PSPSPTNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSAL 192 P P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SAL Sbjct: 132 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 191 Query: 193 ALAVLAERAGIPAGVFSVVTGTPKEIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIK 252 A + AG+PAGVF++V G+ E+G ++++P V IS TGST G L+ Q +A ++K Sbjct: 192 LFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 253 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLV 312 +++ ELGG +P +V DADL+ A+ +A+ RN GQ+C R+ V + Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELAL 311 Query: 313 EAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHVADALAKGARVLLG--GKRHALGHGFF 370 + ++KVGD + T GPL N +V+Q + L GAR+L G G+ L G++ Sbjct: 312 ATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYY 371 Query: 371 -EPTVLADVTPAMQVAREETFGPMAPLFRFKTDDEALSLANDTEFGLASYFYSRDIGRIW 429 PT+ ++V M +A+EE FGP+ + + T +EA+++ANDT +GL ++ RD ++ Sbjct: 372 ARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVR 431 Query: 430 RVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVK 478 VA ++SG V +N + APFGG KQSG GRE G+++Y+ VK Sbjct: 432 AVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVEGMEEYMEVK 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,507,821 Number of extensions: 64481 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 21 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)