BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0871.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 196 4e-51 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 195 5e-51 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 188 6e-49 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 185 6e-48 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 169 4e-43 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 196 bits (497), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 136/412 (33%), Positives = 217/412 (52%), Gaps = 29/412 (7%) Query: 7 RHQPVRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFLA 66 Q +A+ + ++G+ +E+YDF+IYATA++L+F +LFFP + +AS T+ VG++A Sbjct: 10 HQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVA 69 Query: 67 RPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQG 126 RP+G V G +GD GRKK L+ + +MG T+ +GLLPTY +G+WAP LV +R QG Sbjct: 70 RPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQG 129 Query: 127 IAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMF-KLVSGMDNAVFLD 185 AV GE GA M EHSP RR + +SF G AG ILA +F L M F Sbjct: 130 FAVAGEISGASSMILEHSP-FGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNV 188 Query: 186 WGWRLPFLMSALLLVVGFAIRLSVQESPDF----LDEQRRRASAPLAAKPASAPLAEVLR 241 WGWR+PFL+S L+++ G+ IR +V E+P F +E +RR +P+ + R Sbjct: 189 WGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRR-----------SPVVDAFR 237 Query: 242 G-APGLLVLAIGA--NVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFIT 298 +P +L + A NV+ + F + V + + + L +V +++ + Sbjct: 238 DCSPNMLRVVCMALMNVIPVVATIFGAAYA-VQPAYGIGFAKDVYLWIPVVGNIVAVLVI 296 Query: 299 PLAAHLARKVGNHRFLVMMSALSIVSPYAMFSLVDLGSLASVTLGVAIAVLFIG----AY 354 P L+ ++G +++ S L+ + +A + S+ +V L +++L G Y Sbjct: 297 PYVGKLSDRIGRRPPIIVGSLLAGLFAFAYLYAI---SIRNVELAFVMSILMWGVVYQGY 353 Query: 355 YAVIAGFVSDAFPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAEKFQGHWW 406 AV F + FP R R + +++A Q G + P L T +A + W Sbjct: 354 NAVFPSFYPELFPARARVSAMAIA-QNLGTAITAMLPALFTAVAPPGSSNIW 404 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 195 bits (496), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 127/384 (33%), Positives = 205/384 (53%), Gaps = 14/384 (3%) Query: 7 RHQPVRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFLA 66 Q RA +A +G +EW+DF +Y + A++ KLFFPT N L + TF VGF Sbjct: 16 NQQRRRAIVATVIGNGLEWFDFTVY-SFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 67 RPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQG 126 RP+GG + G DR+GRK +L +T+L+M T +IGL PTY SIG++AP+ +V R +QG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 127 IAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGMDNAVFLD- 185 + GGE G A +E++P+R R + +S+ Q ++L + V+ +A L Sbjct: 135 FSAGGEMGSATAFLTEYAPER-ERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALAS 193 Query: 186 WGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRGAPG 245 WGWRLPFL+ ++ VG+ IR ++E+P FL+E+ + S PL EV+R P Sbjct: 194 WGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDS----------PLKEVIRNFPR 243 Query: 246 LLVLAIGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFITPLAAHLA 305 + +L Y +M Y+ + L+L + +V + + P+ LA Sbjct: 244 ETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLA 303 Query: 306 RKVGNHRFLVMMSALSIVSPYAMFSLVDLG-SLASVTLGVAIAVLFIGAYYAVIAGFVSD 364 ++G L +AL ++ Y MF+ ++ SL+++ + + I AY I S+ Sbjct: 304 DRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSE 363 Query: 365 AFPTRIRYTGISMAYQVSGALFGG 388 FP ++ TG+S+AY ++ +FGG Sbjct: 364 LFPAKVLSTGLSVAYNLAVTIFGG 387 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 188 bits (478), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 126/400 (31%), Positives = 205/400 (51%), Gaps = 19/400 (4%) Query: 6 PRHQPVRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFL 65 P+ R +AA VG +EWYDF +Y SA++ +LFFP N + L + TF +GF Sbjct: 3 PQLNKQRQVIAAIVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFF 61 Query: 66 ARPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQ 125 RP+GG + G DR GRK ++ + +++M +I PTYA+IGI API +V R +Q Sbjct: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 Query: 126 GIAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGMDNAVFLD 185 G A GGE+ + E +P +R S+ +G + M LV+ + LD Sbjct: 122 GFATGGEYSSSTAFLVESAPAH-KRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALD 180 Query: 186 -WGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRGAP 244 WGWRLPF++ L+ VG IR + E+ DFL+ + +AP+ L +V++ Sbjct: 181 SWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIK-------LLDVVKTNL 233 Query: 245 GLLVLAIGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQF----FITPL 300 +++++G + G FY +LV + Q GL L+ +V ++ + P Sbjct: 234 RGVLVSMGQVINGTVAFY----VVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPF 289 Query: 301 AAHLARKVGNHRFLVMMSALSIVSPYAMFSLVDLG-SLASVTLGVAIAVLFIGAYYAVIA 359 A L+ ++G L+ S ++ Y +F V SL + + + + IGA + + Sbjct: 290 AGALSDRLGRRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMP 349 Query: 360 GFVSDAFPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAE 399 +S+ FPTR+R TG+++AY ++ LFGG P + T L + Sbjct: 350 TALSEQFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTK 389 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 185 bits (470), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 127/394 (32%), Positives = 200/394 (50%), Gaps = 18/394 (4%) Query: 9 QPVRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVGFLARP 68 Q RA +++ +G +EW+D IY A A+V K FFPT + L +F TF V F RP Sbjct: 16 QARRAIISSSIGNALEWFDILIYG-AFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRP 74 Query: 69 LGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRFIQGIA 128 LG V G DR GRK +L++++++M T +I +P+YASIG+ AP + + IQG + Sbjct: 75 LGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFS 134 Query: 129 VGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATLMFKLVSGMDNAVFL-DWG 187 GGE+G + EH+P RR F +S+ L+LA L +++ M L WG Sbjct: 135 AGGEFGSSTAFLVEHAPH--RRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWG 192 Query: 188 WRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRGAPGLL 247 WR+PF+ L+ G IR + E+P+F + + +APL + L Sbjct: 193 WRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEK----------TNAPLRDTFAHQKMRL 242 Query: 248 VLAIGANVLGIAGFYFTNTFMLVYTTQYLQLQRGLVLNGLLVVSVMQFFITPLAAHLARK 307 ++ G+ ++ Y + ++ Y + L L + V ++ F +PL L+ K Sbjct: 243 LIGAGSVIMATVSVYLS-LYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDK 301 Query: 308 VGNHRFLVMMSALSIVSPYAMFSLV--DLGSLASVTLGVAIAVLFIGAYYAVIAGFVSDA 365 +G F++ SAL IV Y MF + G L + L I VL + Y+A + ++D Sbjct: 302 IGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVL-MTMYFAAMPALLADI 360 Query: 366 FPTRIRYTGISMAYQVSGALFGGLTPLLGTVLAE 399 FP R TG+S+AY ++ +FGG L+ T L + Sbjct: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLID 394 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 169 bits (428), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 110/280 (39%), Positives = 165/280 (58%), Gaps = 16/280 (5%) Query: 4 TPPRHQPVRASLAAFVGTMIEWYDFYIYATASALVFGKLFFPTTNGFYGTLASFGTFAVG 63 TP + + AS +GT+ EWYDFY+Y + +A++ K FF T+ + + FA G Sbjct: 14 TPEEKKVIFASS---LGTVFEWYDFYLYGSLAAII-AKQFFAGTDPNTAFIFALLAFAAG 69 Query: 64 FLARPLGGAVFGTIGDRLGRKKSLVITLLMMGSVTVVIGLLPTYASIGIWAPIALVALRF 123 F+ RP G VFG +GD +GRK + ++T+L+MG+ T ++G+LP Y SIGI API L+ LR Sbjct: 70 FIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRI 129 Query: 124 IQGIAVGGEWGGAVLMASEHSPDRSRRTFLASFAQLGSPAGLILATL-MFKLVSGMDNAV 182 +QG+A+GGE+GGA +EH+P+ R +F A + Q + GL L+ L + + + + A Sbjct: 130 LQGLALGGEYGGAATYVAEHAPEGRRGSFTA-WIQTTATMGLFLSLLVILGVRTAVGEAA 188 Query: 183 FLDWGWRLPFLMSALLLVVGFAIRLSVQESPDFLDEQRRRASAPLAAKPASAPLAEVLRG 242 F DWGWR+PFL+S LLL + IR+S+ ESP FL + K + APL E Sbjct: 189 FADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAE-------GKTSKAPLTEAFGQ 241 Query: 243 APG--LLVLAIGANVLGIAGFYFTNTFM-LVYTTQYLQLQ 279 L++LA+ G A ++T F L + TQ L++ Sbjct: 242 WKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVD 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,280,996 Number of extensions: 51168 Number of successful extensions: 387 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 337 Number of HSP's successfully gapped: 18 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)