BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0867.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 431 e-122 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 140 1e-34 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 103 2e-23 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 103 2e-23 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 103 2e-23 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 431 bits (1108), Expect = e-122, Method: Compositional matrix adjust. Identities = 240/336 (71%), Positives = 264/336 (78%) Query: 2 TEKACAPSEEITIEAAQWIVRLSAGNAAQQRQAAEEFARWKSESRQHAQAAHELHGVLEQ 61 T + A SE+IT AA WIVRLSAG+AAQQ++ A FARW+ +SRQHAQAA EL GVL Sbjct: 5 TAQPGASSEDITAMAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAH 64 Query: 62 INSLLDTSNGDPVPLCAGLKTAMTQPRLPDRMRRAVATLALICGIGLPLWLTYQAYPADY 121 LL ++ GDP PL AGL+ AM P R RRAVA LAL C IGLPLWL QAYPA Y Sbjct: 65 CEGLLHSAQGDPSPLLAGLEAAMAPSPRPGRGRRAVAALALACCIGLPLWLGLQAYPARY 124 Query: 122 LLADMRTATGAWAAQTLEDGSRITLGTRSAVNFRFDARHRTVELVKGEILVEVARDRARP 181 LLADMRTATG W A+TL DGSR+ L SA NF FDA+ RTVELV+GE+LVEVARD ARP Sbjct: 125 LLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPARP 184 Query: 182 FLIQTTDGSIEALGTRFLVRRDDSGTVVSMLESKVSIHAAGQQAQTPVILAAGQRIRLHG 241 F+IQT DGSI ALGTRFLVRRD +GT+VSMLESKVSI Q P++L AGQR+RLHG Sbjct: 185 FVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQPGADTTQAPLVLEAGQRVRLHG 244 Query: 242 DTVGVPEPIDAAALERAWRSHQLVSRQRPLTELLDELNRYRPGRILYDQAQLKGLEMTVV 301 G E IDAAALERAWR HQLVS RPLTE+LDELNR+RPGRILYDQAQL GL MTVV Sbjct: 245 GAAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVV 304 Query: 302 LPLDDTQRALQLLVDCIPGLRVRQFTPYLTYIDLVP 337 LPLDDTQRALQLLVD +PGLRVRQFTPYLTYIDL P Sbjct: 305 LPLDDTQRALQLLVDGVPGLRVRQFTPYLTYIDLAP 340 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 110/331 (33%), Positives = 160/331 (48%), Gaps = 25/331 (7%) Query: 10 EEITIEAAQWIVRLSAGNAAQQRQAAEEFARWKSESRQHAQAAHELHGVLEQINSLLDTS 69 E I EAA W++ + G R++ + A+W+ S +H +A + E +L Sbjct: 13 EAILTEAATWLMEMHEGPLDAARRS--KLAQWRQRSPEHERAWSRAALLQETFAAL---- 66 Query: 70 NGDPVPLCAGLKTAMTQPRLPDRMRRAVATLALICGIGLPLWLTYQAYPADYLLADMRTA 129 G TA+ Q P R+A+ LA +G WL Y A A + TA Sbjct: 67 -------PPGGATALKQVG-PAGRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIATA 118 Query: 130 TGAWAAQTLEDGSRITLGTRSAVNFRFDARHRTVELVKGEILVEVARDR---ARPFLIQT 186 G L+DGSR+ L T + + F R + L +GEI+V A DR RPFL+ T Sbjct: 119 IGERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVAT 178 Query: 187 TDGSIEALGTRFLVRRDDSGTVVSMLESKVSIHAAGQQAQTPVILAAGQRIRLHGDTVGV 246 GS+ LGTRF VR D V++L+ V I A QT +L +G + R Sbjct: 179 RQGSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQT--VLPSGMQTRFTRSGSAA 236 Query: 247 PEPIDAAALERAWRSHQLVSRQRPLTELLDELNRYRPGRILYDQAQLKGLEMTVVLPLDD 306 P P D + AW L++ + PL + + EL RYR G + D A + GLE++ V PL D Sbjct: 237 PRPCDDT--QAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPA-IAGLEVSGVFPLAD 293 Query: 307 TQRALQLLVDCIPGLRVRQFTPYLTYIDLVP 337 T R L+LL +P +RV+Q T Y ++ ++P Sbjct: 294 TDRVLRLLAQTLP-VRVQQLTRY--WVRVLP 321 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 100/328 (30%), Positives = 148/328 (45%), Gaps = 24/328 (7%) Query: 10 EEITIEAAQWIVRLSAGNAAQQRQAAEEFARWKSESRQHAQAAHELHGVLEQINSLLDTS 69 E+I +A +W+++L G+ +AA F +WK+ +HA A L ++Q + Sbjct: 5 EKIVEQAIEWMLQLEQGDLPPGERAA--FEQWKAADPRHAMAYANLSQSVQQFD------ 56 Query: 70 NGDPVPLCAGLKTAMTQPRLPDRMRRAVATLALICGIGLPLWLTY---QAYPADYLLADM 126 VP G + + L + +R A + GL + + P L AD+ Sbjct: 57 ----VPRQVGAAPVILRNTLQQKSKRRKALKRIAALAGLAVGAAALLDRVTPVAALTADL 112 Query: 127 RTATGAWAAQTLEDGSRITLGTRSAVNFRFDARHRTVELVKGEILVEVARDRARPFLIQT 186 T TG TL DGS ITL RSA++ R V L++GEI V++A A PF I+T Sbjct: 113 HTGTGQRQRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAAANALPFSIET 172 Query: 187 TDGSIEAL-GTRFLVRRDDSGTVVSMLESKVSIHAAGQQAQTPVILAAGQRIRLHGDTVG 245 GS+ A GT L + +V+ + + GQ A LAAGQR D +G Sbjct: 173 VHGSVSATAGTLMLGLQPLRTDLVAQQAAALLSTRNGQHA----TLAAGQRTWFDQDEIG 228 Query: 246 VPEPIDAAALERAWRSHQLVSRQRPLTELLDELNRYRPGRILYDQAQLKGLEMTVVLPLD 305 P A E +W + L E++ L YR G + D A + L ++ PLD Sbjct: 229 APRA--AGGAENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLDPA-VAQLRISGAFPLD 285 Query: 306 DTQRALQLLVDCIPGLRVRQFTPYLTYI 333 DT RAL L +P + V + +PY I Sbjct: 286 DTDRALSALASALP-ITVNRVSPYWVSI 312 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 95/308 (30%), Positives = 137/308 (44%), Gaps = 19/308 (6%) Query: 36 EEFARWKSESRQHAQAAHELHGVLEQINSLLDTSNGDPVPLCAGLKTAMTQPRLPDRMRR 95 E + W+S H +A + V ++ +L P+ A L A P R+ Sbjct: 42 EAWEAWRSAHPDHERAWRRIESVRGRLQALAS-------PVNAALAQAALTP--SGNRRQ 92 Query: 96 AVATLALICGIGLPLWLTYQAYPADYLLADMRTATGAWAAQTLEDGSRITLGTRSAVNFR 155 + TLAL+ G W ++ P AD+RT G LEDGS++ L T SAVN Sbjct: 93 VIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLA 152 Query: 156 FDARHRTVELVKGEILVEVAR-DRARPFLIQTTDGSIEALGTRFLVRRDDSGTVVSMLES 214 F A R + L+ GE+LV + RPFL++T G ALGTR+ VR+ T VS+ E Sbjct: 153 FSAEERRLRLISGELLVTTGKAGGGRPFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEG 212 Query: 215 KVSIHAAGQQAQTPVILAAGQRIRLHGDTVGVPEPIDAAALERAWRSHQLVSRQRPLTEL 274 V + Q AQ +L AGQ+ VG D + AW +V+R L Sbjct: 213 AVRL-VPRQAAQQARVLQAGQQAAFSAQGVGDVTTADETRM--AWTDGVIVARGMRLDAF 269 Query: 275 LDELNRYRPGRILYDQAQLKGLEMTVVLPLDDTQRALQLLVDCI---PGLRVRQFTPYLT 331 L EL RY R+ A + G+ ++ P+ D+ + L + LR R + Sbjct: 270 LAELGRYTTDRLSCAPA-VSGVRISGSYPVADSDAVIATLAAALGLQAQLRQRFWGGRQW 328 Query: 332 YIDLVPTP 339 Y L P+P Sbjct: 329 Y--LAPSP 334 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 101/317 (31%), Positives = 150/317 (47%), Gaps = 20/317 (6%) Query: 15 EAAQWIVRLSA--GNAAQQRQAAEEFARWKSESRQHAQAAHELHGVLEQINSLLDTSNGD 72 +A W+V+L A G+AA + A W++ +H Q EL EQ L+ Sbjct: 15 QAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRLVAL---- 70 Query: 73 PVPLC---AGLKTAMTQPRLPDRMRRAVATLALICG-IGLPLWLTYQAYPADYL-LADMR 127 PVP LK + + RR + L G I L QA + L A Sbjct: 71 PVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGASYA 130 Query: 128 TATGAWAAQTLEDGSRITLGTRSAVNFRFDARHRTVELVKGEILVEVARD----RARPFL 183 T TG L DG+ + L T S+V RFD R + L +GEI ++ D RPF Sbjct: 131 TRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHRPFW 190 Query: 184 IQTTDGSIEALGTRFLVRRDDSGTVVSMLESKVSIHAAGQQAQTPVILAAGQRIRLHGDT 243 +QT ++ALGTRF VR+ GT +++LE +V++H AG P++ G+ + +H D Sbjct: 191 VQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGVD---PLVAQPGEIVDIHPDG 247 Query: 244 VGVPEPIDAAALE-RAWRSHQLVSRQRPLTELLDELNRYRPGRILYDQAQLKGLEMTVVL 302 V A+A++ AW LV RQ L +++ +L RYR G + D A + +++ V Sbjct: 248 RLVRRAARASAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPA-VADWQVSGVF 306 Query: 303 PLDDTQRALQLLVDCIP 319 L DT AL+ L +P Sbjct: 307 QLQDTDVALEALAQALP 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,032,549 Number of extensions: 41818 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 5 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)