BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0865.p (244 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309390|ref|YP_003773482.1| glutamine amidotransferase [Her... 65 5e-12 gi|300309844|ref|YP_003773936.1| glutamine amidotransferase [Her... 62 6e-11 >gi|300309390|ref|YP_003773482.1| glutamine amidotransferase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 47/149 (31%), Positives = 71/149 (47%), Gaps = 8/149 (5%) Query: 50 IVILGSDHSVND--ALPWIAAEKSLLEDALARDVPVLGHCFGAQLLARAMGAKVTRNIC- 106 +++LG +V D A PW+ AE + L L D+P LG GAQL+A A+ A+V C Sbjct: 51 LIVLGGPIAVYDTEAYPWLRAEIAWLRARLLEDLPTLGLGLGAQLMAAALHARVYPGSCG 110 Query: 107 PNIGWGQVWITPPAQQRMRLPRL----AWLFNWHYDTFEIPTGAVRTMYGSHCLNKGFLR 162 IGW ++ P + L +L + +WH DTF++P GA ++ F Sbjct: 111 KEIGWSRLQAGPHLDEHAWLRQLVDQETRVLHWHGDTFDLPRGARHLASSDMYPHQAFAW 170 Query: 163 GRQ-WGFQGHMEVTEESIRRWCASGREEL 190 GR Q H EV ++ +W E+ Sbjct: 171 GRHCLALQFHPEVDVRTVEQWMLGHAHEI 199 >gi|300309844|ref|YP_003773936.1| glutamine amidotransferase [Herbaspirillum seropedicae SmR1] Length = 238 Score = 61.6 bits (148), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 60/203 (29%), Positives = 91/203 (44%), Gaps = 16/203 (7%) Query: 1 MRPIAIFQHEATQGPGVLLEHLQQHALPYRL-YHSAAQDDVPRDASQFAGIVIL--GSDH 57 M+ +H + G+L L H YRL Y A + A++ A ++I+ G Sbjct: 1 MKEAIALRHVHFEDVGILDSLLASHG--YRLRYIDATIEQADTAAAEQADLLIVLGGPIG 58 Query: 58 SVNDAL-PWIAAEKSLLEDALARDVPVLGHCFGAQLLARAMGAKVTRNICPNIGWGQVWI 116 + ++ L P++ E L+ L + P+LG C GAQL+ARA+GA+V IG+ + + Sbjct: 59 AYDEGLHPFLLRELDLIARRLQQQRPLLGICLGAQLIARALGAEVAPMGLKEIGYAPLDL 118 Query: 117 TPPAQQRMRLPRLAWL-----FNWHYDTFEIPTGAVRTMYGSHCLNKGFLRGRQ-WGFQG 170 + RL LA L +WH D F IP GA C ++ F G Q Sbjct: 119 STEG----RLSPLAALEGQPVLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQF 174 Query: 171 HMEVTEESIRRWCASGREELLRA 193 H+E I +W EL +A Sbjct: 175 HLEADPARINQWLVGHACELGQA 197 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 848,562 Number of extensions: 36771 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 2 Length of query: 244 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 161 Effective length of database: 1,226,865 Effective search space: 197525265 Effective search space used: 197525265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)