BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0861.p (241 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 387 e-109 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 123 1e-29 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 122 4e-29 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 110 8e-26 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 107 8e-25 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 387 bits (994), Expect = e-109, Method: Compositional matrix adjust. Identities = 203/241 (84%), Positives = 219/241 (90%) Query: 1 MQFGGTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLESLREEIGGRSVQVDLAD 60 M F G +VIITGAG+GIGRACA L+AQRGA+VIAL+RTASDL L+EEIG R + VDL+D Sbjct: 1 MSFAGKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVIAVDLSD 60 Query: 61 PAAARRAMAEAGSADYLINSAGINVLQSTLAMSDDAYEAVLGVNLRAALITCQAFAQARI 120 PAAARRAMAEAG+AD+LINSAGINVL+S L MSD+ YEAVLGVNLRAALITCQAFA+ RI Sbjct: 61 PAAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRI 120 Query: 121 AAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL 180 A GGGGA+VNITSIAGHRGF +HLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL Sbjct: 121 AQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL 180 Query: 181 TELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSEDAAMMTGAVVPVDGGFLA 240 TELAEAAWSDPAKS PMM RHP RFAS EDVARSIAMLLS DAAM+ GAVVPVDGGFLA Sbjct: 181 TELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLA 240 Query: 241 V 241 V Sbjct: 241 V 241 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 123 bits (309), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 88/242 (36%), Positives = 124/242 (51%), Gaps = 19/242 (7%) Query: 5 GTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLESLREEIGGRSV---QVDLADP 61 G + ++TG +GIG A AR + GA VIA A EE G V ++++A Sbjct: 10 GKTALVTGGTQGIGAAVARQLVALGARVIA----AGLPPFAHEEFAGSEVTLVEMNVASE 65 Query: 62 AAARRAMAEAGSADYLINSAGINVLQSTLAMSDDAYEAVLGVNLRAALITCQA----FAQ 117 + R A+A D ++N AGI ++ + + +E V+ VNL + C A Q Sbjct: 66 ESVRAALAGLTQLDIVVNCAGI--IKRGAELETEVFEQVIAVNLTGTMRVCAAARELLRQ 123 Query: 118 ARIAAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPT 177 +R G IVN S+ G Y+ASK G+ T+ LA GIRVNAVAP Sbjct: 124 SR------GCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 178 ITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSEDAAMMTGAVVPVDGG 237 T L +A DP ++ P++ R PL R+ EDV + +A L S A+ MTGA+VPVDGG Sbjct: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 Query: 238 FL 239 +L Sbjct: 238 YL 239 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 122 bits (305), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 84/248 (33%), Positives = 135/248 (54%), Gaps = 21/248 (8%) Query: 5 GTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLESLREEIG------GRSVQVDL 58 G ++TGA +GIGRA A +A++GA+VI + + S ++ E + GR +++ Sbjct: 8 GQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVLNV 67 Query: 59 ADPAAA----RRAMAEAGSADYLINSAGINVLQSTLAMSDDAYEAVLGVNL----RAALI 110 D A+ + + GS L+N+AGI Q + M D+ +++V+ NL R + Sbjct: 68 NDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRA 127 Query: 111 TCQAFAQARIAAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLARELGPHGIR 170 + +AR G I+NITS+ G G + YAA+KAG+ G +R LARE+G I Sbjct: 128 VLRGMMKART-----GRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNIT 182 Query: 171 VNAVAPTITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSEDAAMMTGA 230 VN VAP T++ A + +SA ++ + PL R +AE++A ++A L S AA +TG Sbjct: 183 VNCVAPGFIDTDMTRAL--NEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 Query: 231 VVPVDGGF 238 + V+GG Sbjct: 241 TLNVNGGM 248 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 110 bits (276), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 84/244 (34%), Positives = 131/244 (53%), Gaps = 15/244 (6%) Query: 7 SVIITGAG-KGIGRACARLMAQRGAEVIALSRTASDLESLREEIGGRSVQV--DLADPAA 63 +VI GAG G+G A ARLMA++GA V+ L +D + IG + + + D+ D A+ Sbjct: 8 AVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDKAS 67 Query: 64 ARRA----MAEAGSADYLINSAGINVLQSTLAMSDDAYEAVLGVNLRAALITCQAFAQAR 119 A + + G D L+N+AGI TL ++ Y+ +L V+LR L QA A Sbjct: 68 CETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQAVLPA- 126 Query: 120 IAAGGGGAIVNITSIAGHRGFA----EHLCYAASKAGLEGATRVLARELGPHGIRVNAVA 175 + A G+I+ I+S++ RG H Y+A+KAG+ G R +ARE GP GIRVN++ Sbjct: 127 MRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFGPEGIRVNSIT 184 Query: 176 PTITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSEDAAMMTGAVVPVD 235 P + T++ + ++ K A + PL R A+D+ + L S+ + TG + V+ Sbjct: 185 PGLIGTDIIKGKLTEE-KKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVN 243 Query: 236 GGFL 239 GG L Sbjct: 244 GGML 247 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 107 bits (267), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 122/247 (49%), Gaps = 11/247 (4%) Query: 2 QFGGTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLESLREEIGGRSVQVDLADP 61 + G +V+IT A +GIGRA L A+ GA VIA + L+ L G + +D+ D Sbjct: 7 RLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVETHLLDVTDD 66 Query: 62 AAARRAMAEAGSADYLINSAGINVLQSTLAMSDDAYEAVLGVNLRAALITCQAFAQARIA 121 AA + +A+ G+ D L N AG + L D A++ +N +A T +A +A Sbjct: 67 AAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAVLPGMLA 126 Query: 122 AGGGGAIVNITSIAGH-RGFAEHLCYAASKAGLEGATRVLARELGPHGIRVNAVAP-TI- 178 G+IVNI S A +G A Y ASKA + G T+ +A + GIR NA+ P TI Sbjct: 127 K-KAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICPGTIE 185 Query: 179 -------TLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSEDAAMMTGAV 231 T+ E S+ A + R P+ R AE+VA L S+++ TG++ Sbjct: 186 SPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNFTTGSI 245 Query: 232 VPVDGGF 238 +DGG+ Sbjct: 246 HMIDGGW 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 620,618 Number of extensions: 20668 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 52 Length of query: 241 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 158 Effective length of database: 1,226,865 Effective search space: 193844670 Effective search space used: 193844670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)