BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0859.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbas... 474 e-135 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 95 4e-21 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 91 1e-19 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 87 2e-18 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 85 5e-18 >gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 243 Score = 474 bits (1220), Expect = e-135, Method: Compositional matrix adjust. Identities = 228/243 (93%), Positives = 236/243 (97%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQVT 60 MTQSLAGKIAVITGAASGIGLATTEKLLAN TVVMVDWNAKAL+ELAARLGP+ IPQVT Sbjct: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQVIPQVT 60 Query: 61 NLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNV 120 NLLDA+SCAAM PE+L+KV HIDILYCNAGTYIGGELVDT+ EAIDKMLNLNVNAVMKNV Sbjct: 61 NLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV 120 Query: 121 HAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 HAVAPHM+ RK+GDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ Sbjct: 121 HAVAPHMMERKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF Sbjct: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 Query: 241 DRV 243 DRV Sbjct: 241 DRV 243 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 95.1 bits (235), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 74/244 (30%), Positives = 119/244 (48%), Gaps = 8/244 (3%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARL---GPKAIP 57 M+ ++ K+ VITGA+SG+G T L + A +V+ + LE+L A + G +AI Sbjct: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 60 Query: 58 QVTNLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVM 117 T++ D A+V + Q ID+L NAG + E D+M+++NV V+ Sbjct: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 Query: 118 KNVHAVAPHMIARKTGDIIVTCSVAG-HFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGV 176 V A P A+ +G II SVAG VY+ +K+A+ + +R + P GV Sbjct: 121 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEA-PDGV 179 Query: 177 RVGQVSPGPVISALLADWPEENLRKAK---ESGSLIEPSEVADAVEYMLTRNRNVTIRDM 233 R +SPG V S L ++ +A + I VA A+ Y + + NV I ++ Sbjct: 180 RTTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEV 239 Query: 234 LVLP 237 ++ P Sbjct: 240 VLRP 243 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 90.5 bits (223), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/209 (32%), Positives = 100/209 (47%), Gaps = 16/209 (7%) Query: 2 TQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQVTN 61 T LAGK +IT AA GIG A+TE A V+ D + L+ELA G + T+ Sbjct: 5 TGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVE-----TH 59 Query: 62 LLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSAEAIDKMLNLNVNAVMKNVH 121 LLD AA + ++ K+ ID+L+ AG G +++ +A D NLN A+ + Sbjct: 60 LLDVTDDAA-IKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIR 118 Query: 122 AVAPHMIARKTGDIIVTCSVAGHFPTYWEPV-YAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 AV P M+A+K G I+ S A Y SK A+ +++ + G+R Sbjct: 119 AVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNA 178 Query: 181 VSPGPVISALLADWPEENLR---KAKESG 206 + PG + S P N R +AKE+G Sbjct: 179 ICPGTIES------PSLNQRISTQAKETG 201 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 68/237 (28%), Positives = 105/237 (44%), Gaps = 26/237 (10%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARLGPKAIPQVT 60 M + K+ V+TG + GIGLAT + A A V + A LE+ +G A+ Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 62 Query: 61 NLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSA--------EAIDKMLNLN 112 + D + V +D+L+ NAGT T A E D + NLN Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGT------ASTEAKPLGMIGEEDFDGLFNLN 116 Query: 113 VNAVMKNVHAVAPHMIARKTGDIIVTCSVAG--HFPTYWEPVYAGSKWAITCFVQTMRRQ 170 V ++ +V P M + G I++ SVAG FP + +Y SK A+ F ++ Sbjct: 117 VRGLLFSVQKALPLM--SQGGAIVLNGSVAGVKGFP--GQSLYNASKAAVRSFARSWTAD 172 Query: 171 MIPHGVRVGQVSPGPVISALLADWPEEN------LRKAKESGSLIEPSEVADAVEYM 221 + G+RV V+PG + L+ D+ E L++ G L EP E+ AV ++ Sbjct: 173 LKERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFL 229 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 85.1 bits (209), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 68/237 (28%), Positives = 109/237 (45%), Gaps = 26/237 (10%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANDATVVMVDWNAKALEELAARL-----GPKA 55 M + L GKIA++TG SGIGLAT ++L A A V + L+ A + G +A Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 Query: 56 IPQVTNLLDADSCAAMVPEVLQKVAHIDILYCNAGTYIGGELVDTSA---EAIDKMLNLN 112 V + LD + ++ + +DIL+ NAG GG+++ A E D++ N Sbjct: 61 DASVLSELD-----TVYAQIAKSAGRLDILFANAG---GGDMMPLGAITEEHFDRIFGTN 112 Query: 113 VNAVMKNVHAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMI 172 V V+ V P ++ +I+T S T VY+ SK A+ F ++ + Sbjct: 113 VRGVLFTVQKALPLLV--DGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLK 170 Query: 173 PHGVRVGQVSPGPVISALLADWPEENLRKAKES--------GSLIEPSEVADAVEYM 221 +RV VSPGP+ + L + ++ R+ G L EP E+ AV ++ Sbjct: 171 DRAIRVNVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFL 227 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 728,380 Number of extensions: 29165 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 36 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)