BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0841.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 495 e-141 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 103 2e-23 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 96 3e-21 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 95 5e-21 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 94 1e-20 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 495 bits (1274), Expect = e-141, Method: Compositional matrix adjust. Identities = 238/258 (92%), Positives = 248/258 (96%) Query: 1 VDLNLQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARSEPEPQFWSRLTALQPRAAWF 60 +DLNLQ+KVVIVTGGASGIG AIS+QLAAEGAIPVVFARS PE QFW RLTA+QPRAA F Sbjct: 1 MDLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPESQFWERLTAVQPRAALF 60 Query: 61 QLELQDAARCGEAVAQTVARFGRLDGLVNNAGVNDSVGLDAGRDEFVASLERNLIHYYVM 120 QLELQD ARC EAVA T+ RFGRLDGLVNNAGVNDSVGL+AGRDEFVASLERNLIHYYVM Sbjct: 61 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM 120 Query: 121 AHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 AHYCVPHLKATRGAI+NVSSKTA+TGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA Sbjct: 121 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 Query: 181 LIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTT 240 LIPAEVMTPLYEKWIATF+HPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLS RSSHTT Sbjct: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 Query: 241 GQWVFVDGGYTHLDRALT 258 GQW+FVDGGYTHLDRALT Sbjct: 241 GQWIFVDGGYTHLDRALT 258 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 76/246 (30%), Positives = 124/246 (50%), Gaps = 9/246 (3%) Query: 8 KVVIVTGGASGIGGAISMQLAAEG-AIPVVFARSEPEPQFWSRLTALQPRAAWFQLELQD 66 +V +VTGGA GIG +IS QLAA+G + V + + L A RAA Q++L Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 67 AARCGEAVAQTVARFGRLDGLVNNAGVNDSVG-LDAGRDEFVASLERNLIHYYVMA-HYC 124 A A +GR D LVNNAGV + LD + ++ ++ N+ + H Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 125 VPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPA 184 +K G I+N+SS + + + Y SK A + LTR+ A L G+ VN++ P Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 185 EVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQWV 244 V TP+ + H ++ ++ +P+ +R+ T++E+A +L + S++ TG + Sbjct: 189 PVDTPMTQAM-----HSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYITGHVI 242 Query: 245 FVDGGY 250 VDGG+ Sbjct: 243 PVDGGF 248 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 95.9 bits (237), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 123/250 (49%), Gaps = 13/250 (5%) Query: 5 LQEKVVIVTGGASGIGGAISMQLAAEGA-IPVVFARSEPEP-QFWSRLTALQPRAAWFQL 62 L KV ++TGG SGIG A+++ A EGA + +V+ E + Q + AL Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 63 ELQDAARCGEAVAQTVARFGRLDGLVNNAGVNDSVGL--DAGRDEFVASLERNLIHYYVM 120 ++ C AV + +GRLD LVNNA + + + + N+ + M Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 121 AHYCVPHLKATRGA-ILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 +P L TRGA I+N +S TA G + Y A+KGA ++ TR + L G+ VN Sbjct: 243 VKEAMPFL--TRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVN 300 Query: 180 ALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHT 239 A+ P + TPL A+F E++ + +PLG R +E+A VFL + +S+ Sbjct: 301 AVAPGPIWTPLIP---ASFS--AEEVASFGKNVPLG-RPGQPDEVAPAYVFLATEGASYM 354 Query: 240 TGQWVFVDGG 249 TGQ + +GG Sbjct: 355 TGQVLHPNGG 364 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 95.1 bits (235), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 120/247 (48%), Gaps = 9/247 (3%) Query: 5 LQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARSEPEPQFWSRLTALQP-RAAWFQLE 63 + KVVIVTG ASGIG A + + + EGA ++ R +L P R A + + Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAA--ALGKVFDSLPPERTAARETD 69 Query: 64 LQDAARCGEAVAQTVARFGRLDGLVNNAGVNDSVGL-DAGRDEFVASLERNLIHYYVMAH 122 + + + V + RFG+LD LV++AGV + + +++ N+ + A Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 123 YCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALI 182 +PHL+ TRG I+NV+S + L N S Y ASKGA +LTR A GVR+NA+ Sbjct: 130 EALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVC 189 Query: 183 PAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQ 242 P+ T + A LD +I LG R E+A + FL S +S G Sbjct: 190 PSLTHTAM----TADMADDPPLLDKFAERIALG-RGADPLEIAAVITFLASPDASFVNGV 244 Query: 243 WVFVDGG 249 + VDGG Sbjct: 245 NLPVDGG 251 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 78/251 (31%), Positives = 127/251 (50%), Gaps = 6/251 (2%) Query: 3 LNLQEKVVIVTGGASGIGGAISMQLAAEGAIPVVFARSEPEP-QFWSRLTALQPRAAWFQ 61 + LQ+KV IVTGG SG G I+ A EGA VV E + + A RA + + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 62 LELQDAARCGEAVAQTVARFGRLDGLVNNAGVN--DSVGLDAGRDEFVASLERNLIHYYV 119 ++ A + +A + FG+LD +VNNAG + L+ DEF N+ ++ Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 120 MAHYCVPHLKATRG-AILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRV 178 A VP+ + G A +N++S + + + Y SKGA ++ ++ AA L D +RV Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 179 NALIPAEVMTPLYEKWIATFEHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSH 238 N + P T L +++ + P+ + + IP+G RF+T E++A+ ++L S + Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENR-KKFVATIPMG-RFSTPEDIANACLYLGSDEAEF 238 Query: 239 TTGQWVFVDGG 249 TG + VDGG Sbjct: 239 VTGVCIEVDGG 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 776,155 Number of extensions: 30559 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 40 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)