BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0813.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 550 e-158 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 148 5e-37 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 88 9e-19 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 74 2e-14 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 550 bits (1416), Expect = e-158, Method: Compositional matrix adjust. Identities = 285/319 (89%), Positives = 299/319 (93%) Query: 1 MTTLSQKQGVAVLGIYVADLAFRAPAMPGLGQTIAGNGFAMGPGGKGSNQAVAAARVGAA 60 MT+ KQGVAVLGIYVADLAFRAP MPGLGQTIAG GFAMGPGGKGSNQAVAAARVGA Sbjct: 1 MTSFPHKQGVAVLGIYVADLAFRAPNMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP 60 Query: 61 VRFISAIGKDSFGDFALSLWRQESIAPHVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAA 120 VRFISAIGKD+FGDFALSLWR+E+IAPHVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAA Sbjct: 61 VRFISAIGKDAFGDFALSLWRRETIAPHVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAA 120 Query: 121 SQLSSQDVQREHQAIATARVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALDFPSQI 180 SQLS QDV+RE QAI A+VFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPAL FP+Q+ Sbjct: 121 SQLSEQDVERERQAIEAAKVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALAFPAQV 180 Query: 181 YGLCDFITPNEHEAALLTGIAIDSVEDARRAADVLLERGVGCALITLGAQGSLLHSRSQS 240 YGLCDFITPNEHEAALLTGI I ++DARRAADVLLERGVGCALITLGAQGSLLHSRSQS Sbjct: 181 YGLCDFITPNEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQS 240 Query: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMAAEQAARLATAVAAISVTRAGTAPSMP 300 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGM+AE+AARLATAVAAISVTR GTAPSMP Sbjct: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMP 300 Query: 301 SRQEALALLQANSGALVQA 319 SRQEALALL ++ L QA Sbjct: 301 SRQEALALLDTHASVLAQA 319 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 148 bits (374), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 113/306 (36%), Positives = 158/306 (51%), Gaps = 18/306 (5%) Query: 10 VAVLGIYVADLAFRAPAMPGLGQTIAGNGFAMGPGGKGSNQAVAAARV---GAAVRFISA 66 + ++G DL R P MP G+T+AG+ F PGGKG+NQAVA AR+ G V ++ Sbjct: 2 IVIIGSVNMDLVLRVPRMPLPGETLAGDRFMTIPGGKGANQAVACARLAAPGTRVAMVAC 61 Query: 67 IGKDSFGDFALSLWRQESIAPHV--RVID---GAPTGAAFIYVNDASGDNAIIVVPGAAS 121 +G D+FG RQ A + R ID G TG A I V DA+ N+I++ GA Sbjct: 62 VGDDAFG----GQMRQSITACGIDDRYIDEVAGEATGIASIMV-DANAQNSIVIAAGANG 116 Query: 122 QLSSQDVQREHQAIATARVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALDFPSQIY 181 +L + ++R I A + + QLE P + +++A A G T V NPAPA P + Sbjct: 117 RLDVERIERARALIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRALL 176 Query: 182 GLCDFITPNEHEAALLTGIAIDSVEDARRAADVLLERGVGCALITLGAQGSL-LHSRSQS 240 D++ NE EAA+L A + ED A L + G ++TLG +G + Q Sbjct: 177 QKIDYLILNEIEAAML---AEEQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQ 233 Query: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMAAEQAARLATAVAAISVTRAGTAPSMP 300 HLPA V+T AGD F G F +A+G +A A A AA+SVTR G S+P Sbjct: 234 RHLPARKV-QAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIP 292 Query: 301 SRQEAL 306 +R E + Sbjct: 293 TRDEVV 298 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 88.2 bits (217), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 106/305 (34%), Positives = 144/305 (47%), Gaps = 23/305 (7%) Query: 26 AMPGLGQTI-AGNGFAMGPGGKGSNQAVAAARVGAAVRFISAIGKDSFGDFALSLWRQES 84 AM Q I A F G GG SN A+AAAR GAA ++SA+G D FG L+LW++E Sbjct: 11 AMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQDLLALWQREE 70 Query: 85 I-APHVRVIDGAPTGAAFIYVNDASGDNAIIVVPG-AASQLSSQDVQREHQAIATAR--- 139 + HV + G TG F+ +DA+G + G AAS ++ + E A A A Sbjct: 71 VDVRHVAIAPGGSTGIYFV-THDAAGHHFHYRRAGSAASAYQAKALPLEAIAAAGALHLS 129 Query: 140 -VFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALD-FP--------SQIYGLCDFITP 189 + + E EA LA + ARA+G TT + L +P + + LCD P Sbjct: 130 GISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEAFRLCDICLP 189 Query: 190 NEHEAALLTGIAIDSVEDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAG 249 + + +LLTG+ ED D LL GV LG +G + + Q + A Sbjct: 190 SWEDVSLLTGL-----EDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQR-RMVAPYPV 243 Query: 250 TVVETAGAGDAFNGAFAAGLAEGMAAEQAARLATAVAAISVTRAGTAPSMPSRQEALALL 309 ++ GAGD F GAF A L G A AAR A AA+S T G +P R + A+L Sbjct: 244 DSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVEAVL 303 Query: 310 QANSG 314 SG Sbjct: 304 AKTSG 308 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 84/289 (29%), Positives = 114/289 (39%), Gaps = 46/289 (15%) Query: 45 GKGSNQAVAAARVGAAVRFISAIGKDSFGDFALSLWRQESIAPHVRVIDGAPTGAAFIYV 104 G +N A+ AR+G V + S +G D+FG F QE + RVI A Sbjct: 37 GAETNVAIGLARLGLKVGWASRVGNDAFGRFIRQRVAQEGVEVS-RVITDMEFRTAIQLK 95 Query: 105 NDASG--DNAIIVVP--GAASQLSSQDVQ-------REHQAI-------ATARVFVTQLE 146 A G D AI AAS LS D R A AT F Q Sbjct: 96 AKAVGGADPAIEYYRKGSAASHLSVDDFDAGYFGAARHLHATGIAPALSATTMAFAHQ-- 153 Query: 147 QPPEAALAGLQIARAAGTTTVFNP--APALDFPSQ---------IYGLCDFITPNEHEAA 195 + R G T F+P P L +PSQ + D++ P E Sbjct: 154 --------AMDFMRGQGKTISFDPNLRPML-WPSQEVMAQQLNALAFKADWVLPGLSEGK 204 Query: 196 LLTGIAIDSVEDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAGTVVETA 255 +LTG +DAR A LERGV +I LGA+G+ + + + VV+T Sbjct: 205 ILTGH-----DDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQGEGRVAGVPVKEVVDTV 259 Query: 256 GAGDAFNGAFAAGLAEGMAAEQAARLATAVAAISVTRAGTAPSMPSRQE 304 GAGD F +G+ EG+ QA + A ++ G +P+R E Sbjct: 260 GAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEGLPTRAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 804,059 Number of extensions: 28793 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 159 Number of HSP's successfully gapped: 4 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)