BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0807.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310345|ref|YP_003774437.1| UDP-glucose 4-epimerase [Herbas... 466 e-133 gi|300312369|ref|YP_003776461.1| nucleoside-diphosphate-sugar ep... 143 2e-35 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 62 7e-11 >gi|300310345|ref|YP_003774437.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 320 Score = 466 bits (1200), Expect = e-133, Method: Compositional matrix adjust. Identities = 243/319 (76%), Positives = 270/319 (84%), Gaps = 3/319 (0%) Query: 1 MKVLVTGAAGFIGATLLQVLQQAGRIGSHPITQLAALDQQLPATMEGVVRIEGVLQDAQV 60 MK+LVTGA GFIGATL+ +L+Q G+ G I+ LA LD+QL T +VRIEG LQDAQV Sbjct: 1 MKILVTGAGGFIGATLVALLRQRGQAGGRRISSLAVLDRQLGETAPDMVRIEGNLQDAQV 60 Query: 61 QARIAQFGADVCFHLAAVPGGAAEADPALSRRVNLDATLDLFELLAAQAASGGARRAPVV 120 QA+I FGAD+CFHLAA+PGGAAEADPALSRRVNLDATLDLFELLA + A PVV Sbjct: 61 QAQIVAFGADLCFHLAALPGGAAEADPALSRRVNLDATLDLFELLARRDP---ATPPPVV 117 Query: 121 VYASTIAVYGSPLPDPVTPNTPTRPALIYGAHKRACEILLADYTRRGLLDGRSLRLPGIV 180 VYASTIAVYG LPDPVTP TP RPALIYGAHK ACEILLADYTRRGLLDGRSLRLPGIV Sbjct: 118 VYASTIAVYGLDLPDPVTPATPPRPALIYGAHKLACEILLADYTRRGLLDGRSLRLPGIV 177 Query: 181 ARPRAPSGLVSAFMSDLLHALAAGESLSLPVGPQATAWWMSARRCAENLLHAATMDTSLD 240 ARPRA SGLVSAFMS+LLHALAAGE + PVGPQATAWWMSA+RCAENLLHAA+MD + D Sbjct: 178 ARPRAASGLVSAFMSELLHALAAGELFTCPVGPQATAWWMSAQRCAENLLHAASMDPTRD 237 Query: 241 REGEAERVWPLPVLRLSIGQLVQTLSAMYRVDGQALVQYVPQERVEAVFGRYPVLDDSAA 300 EG A RVWPLPVLRLSI Q+V TLSA+Y VDGQALV+Y PQ +VEAVFGRYP LDD AA Sbjct: 238 PEGAAARVWPLPVLRLSIAQVVATLSALYGVDGQALVRYAPQPQVEAVFGRYPRLDDRAA 297 Query: 301 RRLGLQDDGTAEQLVRRAL 319 R+LGL+DDG+ +++V RAL Sbjct: 298 RQLGLRDDGSVQEMVERAL 316 >gi|300312369|ref|YP_003776461.1| nucleoside-diphosphate-sugar epimerase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 143 bits (360), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 105/325 (32%), Positives = 166/325 (51%), Gaps = 19/325 (5%) Query: 1 MKVLVTGAAGFIGATLL-QVLQQA---GRIGS-HPITQLAALDQQLPATMEGVVRIEGV- 54 M +++TG AGF+G+ L Q+LQ+ GR G I ++ LD PA RIE V Sbjct: 1 MHIVITGGAGFLGSRLARQLLQRGTLTGRDGQPQAIKRITLLDVA-PAQGFDDPRIEAVT 59 Query: 55 --LQDAQVQARIAQFGADVCFHLAAVPGGAAEADPALSRRVNLDATLDLFELLAAQAASG 112 + DA V R+ FHLAA+ G AEAD L ++N DAT + E +A + Sbjct: 60 GDIADAAVIERVITAETQSIFHLAAIVSGHAEADFDLGMKINFDATRVILE----RARAL 115 Query: 113 GARRAPVVVYASTIAVYGSPLPDPVTPNTPTRPALIYGAHKRACEILLADYTRRGLLDGR 172 G++ P VV+ S++AV+G LP V NT P YGA K E+L+ DY+R+G +DGR Sbjct: 116 GSK--PRVVFTSSVAVFGGELPAVVPDNTLLMPQSSYGAQKVMGELLVQDYSRKGFIDGR 173 Query: 173 SLRLPGIVARPRAPSGLVSAFMSDLLHALAAGESLSLPVGPQATAWWMSARRCAENLLHA 232 +LR+P I RP AP+ S+F S ++ G+ PV P W MS R+ NL+H Sbjct: 174 ALRMPTISVRPGAPNKAASSFASGIIREPLNGQPSVCPVTPDTRMWLMSPRQAIANLIHG 233 Query: 233 ATMDTSLDREGEAERVWPLPVLRLSIGQLVQTLSAMYRVDGQALVQYVPQERVEAVFGRY 292 ++ + + R + L +++ ++V+ L + + L+++ E ++ + + Sbjct: 234 HEINGA---DLGLARFLSIDGLCVTVKEMVEALEQVAGKEVVQLIEWKEDEAIKRIVNSW 290 Query: 293 P-VLDDSAARRLGLQDDGTAEQLVR 316 P + A+ LG D ++R Sbjct: 291 PGAFEAKRAKALGFTADANFADIIR 315 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 62.0 bits (149), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 58/206 (28%), Positives = 90/206 (43%), Gaps = 25/206 (12%) Query: 19 VLQQAGRIGSHPITQLAALDQQLPATMEGVVR-----IEGVLQDAQV----------QAR 63 V+ AG IGSH + L D + + +R ++ L+D +V Q Sbjct: 10 VIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIMQTD 69 Query: 64 IAQF---GADVCFHLAAVPGGAAEADPALSRRVNLDATLDLFELLAAQAASGGARRAPVV 120 I Q GAD FHLAA+ P + VN+ T ++ E A+ G +R + Sbjct: 70 ILQSALEGADGVFHLAALWLLQCHEYPRSAFDVNVRGTFNVMEACVAK----GVKR---L 122 Query: 121 VYASTIAVYGSPLPDPVTPNTPTRPALIYGAHKRACEILLADYTRRGLLDGRSLRLPGIV 180 VY+S+ +VYG + +P+ + P YGA K A E +L + R L+ LR + Sbjct: 123 VYSSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVGLRYMNVY 182 Query: 181 ARPRAPSGLVSAFMSDLLHALAAGES 206 + G A + +L A+ GES Sbjct: 183 GPRQDYHGAYIAVIMKMLDAIDKGES 208 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,007,305 Number of extensions: 39509 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 3 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)