BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0793.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 823 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 148 8e-37 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 116 3e-27 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 113 2e-26 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 103 2e-23 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 823 bits (2126), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/415 (94%), Positives = 404/415 (97%) Query: 1 MPIDFQLSESQKALQAGARTFAADVLSKVAPTIAALVKPDERFYATRPFYQAMADAGFVK 60 M IDFQLSESQKALQAGAR FAADVL KV PTI LVKPDERFYATRPFYQAMADAGF+K Sbjct: 1 MSIDFQLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIK 60 Query: 61 ALLPESFGGTAMSTLDFALAAEELTAADVNLPSALLGTGLGLHAVVHHGTPEQKQRFLPD 120 ALLPESFGGTA+STLDFALAAEELTA DVN+PSALLGTGLGLHAVVHHGTPEQKQRFLPD Sbjct: 61 ALLPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHAVVHHGTPEQKQRFLPD 120 Query: 121 FIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGK 180 FIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGK Sbjct: 121 FIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGK 180 Query: 181 GAHLYSVICRTDPALPPQQSLAVIMVPGDAPGIEIVSLLDTMGHRATISPRIHFNNVRVP 240 GAHLYSVICRTDPALPPQQSLAVIMVPG+APGIEIV LLDTMGHRATISPR+HFNNVRVP Sbjct: 181 GAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNNVRVP 240 Query: 241 ASNIVGKPGDGITMLEGAFSWTAALIGAACVGVMRAAFDIALKFAHSDRRSGTVPVIEHQ 300 ASNIVGKPGDGI+MLE AFSWTAALIG+ACVGVMR AFDIAL+FA SDRRSGT+PVIEHQ Sbjct: 241 ASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPVIEHQ 300 Query: 301 NVGFMLADIKMRIEAARYLTWKSCHQLDLTEGRSRELAVITKTYCSELCVQVVYDAMRVV 360 NVGFMLADIKMRIEAARYLTWKSCHQLDLTEGRSRELA+ITKTYCSELCVQVVYDAMRVV Sbjct: 301 NVGFMLADIKMRIEAARYLTWKSCHQLDLTEGRSRELAIITKTYCSELCVQVVYDAMRVV 360 Query: 361 GVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRRRQLHTLLRSKDYDAMASAEARG 415 GVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRRRQLHTLLRSK+YDAMASAEARG Sbjct: 361 GVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRRRQLHTLLRSKEYDAMASAEARG 415 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 148 bits (374), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 100/340 (29%), Positives = 172/340 (50%), Gaps = 18/340 (5%) Query: 51 QAMADAGFVKALLPESFGGTAMSTLDFALAAEELTAADVNLPSAL-LGTGLGLHAVVHHG 109 + +A+ G + +PE +GG M + LA EE+ A D + + + L +G Sbjct: 41 KELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYG 100 Query: 110 TPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKH 169 +P QK+++L G L TE G++ + ++T A +GD++++NG K Sbjct: 101 SPTQKEQWLKPLAR-GEMLGCFCLTEPHTGSD------AAAIRTRAERDGDDFILNGTKQ 153 Query: 170 YTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGDAPGIEIVSLLDTMGHRATIS 229 + T+G K A + V TD + ++ ++ +VP D PG + + MG A+ + Sbjct: 154 FITSG-----KHAGVAIVFAVTDRS-AGKKGISCFLVPCDTPGFVVGRSEEKMGQHASDT 207 Query: 230 PRIHFNNVRVPASNIVGKPGDGITMLEGAFSWTAALIGAACVGVMRAAFDIALKFAHSDR 289 +I +N RVPA+ ++GK G+G + I A VG+ RAAF+ A+ +A R Sbjct: 208 VQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQ-R 266 Query: 290 RSGTVPVIEHQNVGFMLADIKMRIEAARYLTWKSCHQLDLTEGR-SRELAVITKTYCSEL 348 S VP+IEHQ V F LAD+ ++AAR + W++ D +GR + A + K + SE Sbjct: 267 ESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQLKD--QGRPCLKEASMAKMFASEA 324 Query: 349 CVQVVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGN 388 ++ DA+++ G YT +P+ + +D +Y+G N Sbjct: 325 AEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGAN 364 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 100/402 (24%), Positives = 183/402 (45%), Gaps = 37/402 (9%) Query: 3 IDFQLSESQKALQAGARTFAADVLSKVAPTIAALVKPDERFYATRPFYQAMADAGFVKAL 62 + F E AL+ FA S++AP A + + D+ ++ + D G + Sbjct: 7 LSFDHGEDIAALREAVAAFAH---SEIAPRAAEIDRSDQ---FPMDLWKKLGDLGVLGIT 60 Query: 63 LPESFGGTAMSTLDFALAAEELTAADVNLPSALLGTGLGLHA------VVHHGTPEQKQR 116 + E +GG + L +A EE++ A SA +G G H+ + +G EQK++ Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRA-----SASVGLSYGAHSNLCVNQIKRNGNEEQKRK 115 Query: 117 FLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTG 176 +LP I G + ALA +E G++ V ++ A +GD +V+NG+K + TNG Sbjct: 116 YLPRLIS-GDFIGALAMSEPNAGSDV------VSMKLRADKKGDRYVLNGSKMWITNGPD 168 Query: 177 WDGKGAHLYSVICRTDPALPPQQSLAVIMVPGDAPGIEIVSLLDTMGHRATISPRIHFNN 236 D + V +TD + + +V G + LD +G R + + + F + Sbjct: 169 AD-----VLVVYAKTDLE-AGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQD 222 Query: 237 VRVPASNIVGKPGDGITMLEGAFSWTAALIGAACVGVMRAAFDIALKFAHSDRRSGTVPV 296 VP N++G G G+ +L + +++ +G+M+A D+ + + H DR+ + Sbjct: 223 CEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVH-DRKQFGQAI 281 Query: 297 IEHQNVGFMLADI---KMRIEAARYLTWKSCHQLDLTEGRS--RELAVITKTYCSELCVQ 351 E Q + LAD+ M +A Y ++C + D + R+ A Y +E Sbjct: 282 GEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATW 341 Query: 352 VVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRR 393 + +A++ +G + Y + YP+ L +DA + + G +RR Sbjct: 342 MAGEAIQSLGGNGYINEYPVGRLWRDAKLYEI-GAGTSEIRR 382 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 102/390 (26%), Positives = 175/390 (44%), Gaps = 21/390 (5%) Query: 3 IDFQLSESQKALQAGARTFAADVLSKVAPTIAALVKPDERFYATRPFYQAMADAGFVKAL 62 +DF+L++ Q A Q AR FAA ++AP A K DE + + GF Sbjct: 1 MDFELNQDQLAFQQSARDFAA---GEMAPFAA---KWDEEAHFPLDVIAKAGELGFCGLY 54 Query: 63 LPESFGGTAMSTLDFALAAEELTAADVNLPSALLGTGLGLHAVVHHGTPEQKQRFLPDFI 122 PE+ GG +S LD + EEL A + + L + + G P K ++ + Sbjct: 55 TPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQ-L 113 Query: 123 EDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGA 182 G ++ + TE G++ + ++T ARLE +V+NG+K + + D Sbjct: 114 AAGRKIGSYCLTEPGSGSD------AASLKTTARLEDGHYVLNGSKAFISGAGSTD---- 163 Query: 183 HLYSVICRTDPALPPQQSLAVIMVPGDAPGIEIVSLLDTMGHRATISPRIHFNNVRVPAS 242 + V+ RT + ++ I+VPG+APG+ MG + + I F+NV+VPA Sbjct: 164 -VLVVMARTGG--EGARGVSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPAD 220 Query: 243 NIVGKPGDGITMLEGAFSWTAALIGAACVGVMRAAFDIALKFAHSDRRSGTVPVIEHQNV 302 +++G+ G G I VG +AA D A + ++ G P+ + Q + Sbjct: 221 HLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGR-PIADFQAL 279 Query: 303 GFMLADIKMRIEAARYLTWKSCHQLDLTEGRSRELAVITKTYCSELCVQVVYDAMRVVGV 362 F LAD++ + AAR + + +LD + + K ++L +V +A+++ G Sbjct: 280 QFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGG 339 Query: 363 DAYTDLYPLAGLMQDALCFPLYDGGNMGVR 392 Y YPL +D + +G N +R Sbjct: 340 YGYIREYPLERYFRDVRVHQILEGTNEIMR 369 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 81/301 (26%), Positives = 139/301 (46%), Gaps = 30/301 (9%) Query: 38 KPDERFYATRPFYQAMADAGFVKALLPESFGGTAMSTLDFALAAEELTAADVNLPSALLG 97 K DE F A+ AG++ AL+P+ +GG+ + + ++ EE+ N A G Sbjct: 31 KIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIMEEINRTGGN-SGACHG 89 Query: 98 TGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARL 157 + ++ HG+PEQK+R+LP RL ++A TE T + + ++T A Sbjct: 90 QMYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVTEPT------TGTDTTRIKTVAVK 143 Query: 158 EGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGDAPGIEIVS 217 +GD +VING K + + D L ++ RT P L + + I +V Sbjct: 144 KGDRYVINGQKVWISRVQHSD-----LMILLARTTP-------LEQVSRKSEGMSIFLVD 191 Query: 218 LLDTMGHRATISP----------RIHFNNVRVPASNIVGKPGDGITMLEGAFSWTAALIG 267 L + +GH + P + F+N+ +PA N++G+ G G + + LI Sbjct: 192 LKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTLIA 251 Query: 268 AACVGVMRAAFDIALKFAHSDRRSGTVPVIEHQNVGFMLADIKMRIEAARYLTWKSCHQL 327 A C+G D A ++A +R P+ +Q V F LA+ + +EAA + +++C Sbjct: 252 AECIGDGYWFIDKATQYAR-ERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRYQACALF 310 Query: 328 D 328 D Sbjct: 311 D 311 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,403,570 Number of extensions: 64311 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 8 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)