BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0783.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 780 0.0 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 149 4e-37 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 143 3e-35 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 128 1e-30 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 125 8e-30 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 780 bits (2014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/453 (92%), Positives = 431/453 (95%) Query: 24 RAGMLPLVALAIGFVMAMLDVTVVNVGLSSIASSLDAPLATLVWIVDGYTLTFAAMLLVG 83 RAGMLPLVALAIGFVMAMLDVTVVNVGLS+IA SLDAPL+TLVWIVDGYTLTFAAMLLVG Sbjct: 26 RAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVG 85 Query: 84 GALADRFGARRLYLTGLLLFVLASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLTH 143 GALADRFGARRLYLTGL LFVLASLLCGAAPNGPFLI ARLLQGLGAAFFMPSSLSLLTH Sbjct: 86 GALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTH 145 Query: 144 VYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLLMARKL 203 VYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGL+MARKL Sbjct: 146 VYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKL 205 Query: 204 IPQVAGHARALHLFSHLAGVVMLAALSFVLIQGPVYGWISPRILVGLAVAVLAAVALVRH 263 IPQV GHARAL+L SHLAGVVMLA LSFVLIQGPVYGW SPRIL GLAVA+ A AL+RH Sbjct: 206 IPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 264 ELRGIAPLLPRALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 323 ELRGIAPLLP+ALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 324 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGAAFTAVAISFRPETPYWILALVCAAAN 383 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVG FTA+A+ F P TPYW+LALVCA+AN Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASAN 385 Query: 384 LGVSTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVQAWSLRNG 443 LG+STAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSV W+LRN Sbjct: 386 LGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNLRNP 445 Query: 444 LAFGVMAAGYGTALWLVWRFVGVVKEQWVTRRA 476 LAFGVMAAGY +ALWLVWRFVGVVKEQW RRA Sbjct: 446 LAFGVMAAGYASALWLVWRFVGVVKEQWQVRRA 478 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 149 bits (377), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 127/430 (29%), Positives = 207/430 (48%), Gaps = 17/430 (3%) Query: 20 SSGHRAG-MLP-----LVALAIGFVMAM--LDVTVVNVGLSSIASSLDAPLATLVWIVDG 71 S H +G +LP L L I FV+ + LD T+V L +I + L W+ Sbjct: 6 DSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVATA 64 Query: 72 YTLTFAAMLLVGGALADRFGARRLYLTGLLLFVLASLLCGAAPNGPFLIAARLLQGLGAA 131 Y LT + + G L D FG + + +++FV AS LCG A + FL+ AR LQG+G Sbjct: 65 YLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGG 124 Query: 132 FFMPSSLSLLTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVP 191 + + + + ++ D RVR R + S + G+A AVGP +GG L LGWR VF N+P Sbjct: 125 MLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLP 184 Query: 192 VGLVGLLMARKLIPQVAGHARALHLFSHLAGVVMLAALSFVLIQG----PVYGWISPRIL 247 +G++ L + +P + A + G ++++ L P G ++ +L Sbjct: 185 IGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRG-VTSGML 243 Query: 248 VGLAVAVLAAVALVRHELRGIAPLLPRALFATPQFAAANGIGFLINFGVYGNLFYLALFL 307 V LAV+V A AL + E R P+LP + A+ + L F ++ L Y L Sbjct: 244 VLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLF 303 Query: 308 QQGRGADALQTGLQLVPMMAVIFFGNLMSGRMSAHW-GPRVPLLLGLS-VGAAFTAVAIS 365 Q G G + GL + P++A I ++++GR+ P + + LG + + A+ + + Sbjct: 304 QGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLC 363 Query: 366 FRPETPYWILALVCAAANLGVSTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGV 425 R ++LA V A G+ +P +T Q A + H A A L + R IG ++G Sbjct: 364 DRVTGKGFLLA-VMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGT 422 Query: 426 ALMGAILHSV 435 A+ G ++ + Sbjct: 423 AITGTLVSQM 432 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 143 bits (361), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 104/292 (35%), Positives = 149/292 (51%), Gaps = 1/292 (0%) Query: 34 AIGFVMAMLDVTVVNVGLSSIASSLDAPLATLVWIVDGYTLTFAAMLLVGGALADRFGAR 93 + G M +LD VV + L ++A L A + + W+V Y L FAA+LL GA ADR+G R Sbjct: 42 SFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRR 101 Query: 94 RLYLTGLLLFVLASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLTHVYEDDRVRAR 153 R+ L G+ LF L SL C AP+ L AR LQG+GAAF + +L+++ H + D+ R R Sbjct: 102 RVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRER 161 Query: 154 MLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLLMARKLIPQVAGH-AR 212 +W +GL + PL+GGV+ LGWR F +N+P L+ + + +P+ R Sbjct: 162 AWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPR 221 Query: 213 ALHLFSHLAGVVMLAALSFVLIQGPVYGWISPRILVGLAVAVLAAVALVRHELRGIAPLL 272 L L+ + L++ LI GP +GW S + L V + + R P+L Sbjct: 222 PLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQRRAAHPML 281 Query: 273 PRALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLVP 324 +LF F AA F L LFLQ RG A QTGL ++P Sbjct: 282 ELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLP 333 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 98/299 (32%), Positives = 151/299 (50%), Gaps = 6/299 (2%) Query: 31 VALAIGFVMAMLDVTVVNVGLSSIASSLDAPLATLVWIVDGYTLTFAAMLLVGGALADRF 90 +++++ M +LD ++ NV + +I+ +L + W++ + A + + G L R Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 91 GARRLYLTGLLLFVLASLLCGAAPNGPFLIAARLLQGLGAAFFMPSSLSLLTHVYEDDRV 150 GA RL++T +LLFVLAS LCG AP P L+AAR+LQGL A +P S SLL Y + Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSK- 145 Query: 151 RARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLV--GLLMARKLIPQVA 208 + L +W T +A GP +GG + W +F IN+PVGL+ G+ +A + Sbjct: 146 SSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETP 205 Query: 209 GHARALHLFSHLAGVVMLAALSFVLIQGPVYGWI-SPRILVGLAVAVLAAVALVRHELRG 267 H + V +AA +L +G W SP I+ +A++A V EL Sbjct: 206 THKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNN 265 Query: 268 IAPLLPRALFATPQFAAANGIGFLINFGV-YGNLFYLALFLQQGRGADALQTGLQLVPM 325 P++ LF FA I I +GV +GNL L +LQQ G ++ +GL P+ Sbjct: 266 DHPVVDLRLFGRRNFAGGT-IAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPI 323 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 133/415 (32%), Positives = 196/415 (47%), Gaps = 29/415 (6%) Query: 33 LAIGFVMAMLDVTVVNVGLSSIASSLDAPLATLVWIVDGYTLTFAAMLLVGGALADRFGA 92 +A GF M LD T+VN L ++A SL P+ + +V YTLT A++ G LADRFG Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 93 RRLYLTGLLLFVLASLLCGAAPNGPFLIAARLLQGLGAAFFMP-SSLSLLTHVYEDDRVR 151 RR+Y +LLF L SLLC A + P L+AAR++QGLG + +P L++L V + Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 152 ARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLLMARKLIPQ--VAG 209 A L S L VGP++GG L+ L W +FLIN+PVG++G+ +P +AG Sbjct: 178 A--LASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAG 235 Query: 210 HARALHLFSHLAGVVMLAALSFVLIQGPVYGWISPRILVGLAVAVLAAVALV---RHELR 266 R L G V+L+A + S R +A+ V+AA+ ++ RH Sbjct: 236 TPRF-----DLLGCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVLGYWRHASV 290 Query: 267 GIAPLLPRALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLVPMM 326 PL LF P F+ + G F L L LQ G L G+ L+P Sbjct: 291 HPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAA 350 Query: 327 AVIFFGNLMSGRMSAHWGPRVPLLLG----LSVGAAFTAVAI-SFRPETPYWIL-ALVCA 380 ++G ++ W + G L V A AI SF TP W L A + Sbjct: 351 --------IAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQ 402 Query: 381 AANLGVSTAIP--AMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILH 433 A G S ++ AM +V ++ K A S + + + + +GV + G +++ Sbjct: 403 LAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,377,443 Number of extensions: 55858 Number of successful extensions: 496 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 472 Number of HSP's successfully gapped: 26 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)