BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0772.p (550 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 958 0.0 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 104 2e-23 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 103 3e-23 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 103 4e-23 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 87 2e-18 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 958 bits (2477), Expect = 0.0, Method: Compositional matrix adjust. Identities = 475/548 (86%), Positives = 503/548 (91%) Query: 1 MHTHDISNQAPDLCDYNLYRNDAALVEAVQREGAHWHDCTLASQGEALGTAEMSELAALA 60 MHTH+I+NQ PDL YNLYR+DAAL EAVQREGAHWHDCTLASQGE LG AEM E+A LA Sbjct: 1 MHTHEITNQVPDLSGYNLYRSDAALAEAVQREGAHWHDCTLASQGELLGGAEMREMAELA 60 Query: 61 NQHSPVLRTHDRTGHRIDVVDFHPAWHTLLEQLRRAGLHALPWMQPGDGAHAARAAGYFL 120 N+H+PVL THDR G RIDVV+FHPAWHTLL+QLRRAGLHALPWMQPGDGAHAARAAGYFL Sbjct: 61 NRHTPVLHTHDRCGRRIDVVEFHPAWHTLLDQLRRAGLHALPWMQPGDGAHAARAAGYFL 120 Query: 121 HAQVEAGSLCPTTMTFAAIPVLQQEAALFAQLRTRLFSREHDGRDLPMMHKRSILIGMGM 180 HAQ+EAGSLCPTTMTFAAI VLQQE ALF QLRTRLFSREHD RDLP+ KRSILIGMGM Sbjct: 121 HAQIEAGSLCPTTMTFAAIAVLQQEPALFEQLRTRLFSREHDARDLPIPQKRSILIGMGM 180 Query: 181 TEKQGGSDVRSNASVAMPADALQQGRGAAYLLTGHKWFFSAPMCDAHLMLARTENGLSCF 240 TEKQGGSDVRSNAS+AMP D L GRGAAYLL+GHKWFFSAPMCDAHLMLARTENGLSCF Sbjct: 181 TEKQGGSDVRSNASIAMPVDPLNDGRGAAYLLSGHKWFFSAPMCDAHLMLARTENGLSCF 240 Query: 241 FVPRWLPDGKRNPVLIQRLKDKLGNRSNSSSEVEFEEALGIMVGEDGRGIPTIIEMANHT 300 FVPRWLPDG RNP+LIQRLKDKLGNRSNSSSEVEFEEA+GIMVG++GRGIPTIIEMANHT Sbjct: 241 FVPRWLPDGTRNPILIQRLKDKLGNRSNSSSEVEFEEAMGIMVGDEGRGIPTIIEMANHT 300 Query: 301 RLDCVIGSTALMRQALVQAIHHARHRNAFGRLLAEQPLMRAVLADLALESEAATMLMLRL 360 RLDC+IGS ALMRQALVQAIHHARHR+AFGR LAEQPLMRAVLADLALESEAATMLMLR+ Sbjct: 301 RLDCIIGSAALMRQALVQAIHHARHRSAFGRRLAEQPLMRAVLADLALESEAATMLMLRV 360 Query: 361 AHAFDAPDDPLQRAWKRIVTPAAKFWICKRALEFTGECMEVWGGNGYVETGPMARLYREA 420 AHAFDAPDDPLQRAWKRI+TPAAKFWICKR LEFTGECMEVWGGNGYVET PMARLYREA Sbjct: 361 AHAFDAPDDPLQRAWKRIITPAAKFWICKRTLEFTGECMEVWGGNGYVETAPMARLYREA 420 Query: 421 PVNSIWEGSGNVMCLDVLRAIEHETEGFALLLMALDDAAAVHPALAQRVRDLKQWLAAPE 480 PVNSIWEGSGNVMCLDVLRA+ ET+G ALLL+ LDDAAA HPAL Q+V LKQ LAAPE Sbjct: 421 PVNSIWEGSGNVMCLDVLRAMARETQGLALLLLELDDAAAGHPALRQQVDALKQMLAAPE 480 Query: 481 EQRQAGARRLVQQLVLALQMMLMLRHAPGESAEAFLASRCDRDGGRVYGTLAVTSLAAQQ 540 E+R+AGARRLVQQLVLALQ MLMLRHAP SA+AFL SR DGGRVYGTL SLAAQ+ Sbjct: 481 EEREAGARRLVQQLVLALQGMLMLRHAPAGSAQAFLESRSQADGGRVYGTLGSASLAAQE 540 Query: 541 AILQRAWP 548 AILQRAWP Sbjct: 541 AILQRAWP 548 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 83/289 (28%), Positives = 129/289 (44%), Gaps = 31/289 (10%) Query: 175 LIGMGMTEKQGGSDVRSNASVAMPADALQQGRGAAYLLTGHKWFFSAPMCD-----AHLM 229 L M +TE Q GSD+ + A+P D +Y L+G K F S D HL+ Sbjct: 161 LATMCLTEAQAGSDLGQLRTRAVPQD------DGSYRLSGDKIFISGGEHDLTPNIVHLV 214 Query: 230 LARTEN------GLSCFFVPRWLPDGKRNPVLIQRLKDKLGNRSNSSSEVEFEEALGIMV 283 LAR + GLS F PR L DG RN V ++ K+G +++ + FE+A +V Sbjct: 215 LARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLV 274 Query: 284 GEDGRGIPTIIEMANHTRLDCVIGSTALMRQALVQAIHHARHR-------NAFGRLLAEQ 336 GE GRG+ + M N RL L A +A +AR R ++ L+ Sbjct: 275 GEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGNDLIVHH 334 Query: 337 PLMRAVLADLALESEAATMLMLRLAHAFD-APDDPLQRAWKR------IVTPAAKFWICK 389 P ++ +L + E A +L D A DP R +TP K ++ Sbjct: 335 PAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTD 394 Query: 390 RALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL 438 + + ++V+GG+GYV + + R+A + I+EG+ + +D L Sbjct: 395 QGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFL 443 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 103 bits (258), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 84/296 (28%), Positives = 140/296 (47%), Gaps = 41/296 (13%) Query: 178 MGMTEKQGGSDVRSNASVAMPADALQQGRGAAYLLTGHKWFFSAPMCDA-----HLMLAR 232 M +TE G+D+ S A P Q G+ Y +TG+K F SA D HL+LAR Sbjct: 165 MCLTEAHCGTDLGLLRSKAEP-----QADGS-YKITGNKIFISAGENDVSANIIHLVLAR 218 Query: 233 TEN------GLSCFFVPRWLPD-----GKRNPVLIQRLKDKLGNRSNSSSEVEFEEALGI 281 + G+S F VP++LP+ G RNP+ +++K+G N++ ++ + A G Sbjct: 219 LPDAPEGSKGISLFLVPKFLPNADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGW 278 Query: 282 MVGEDGRGIPTIIEMANHTRLDCVIGSTAL----MRQALVQAIHHARHRNAFGRLLAEQP 337 ++G+ +G+ + N RL + L + ALV A + R+ G A++P Sbjct: 279 LIGQPHKGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKP 338 Query: 338 ---------LMRAVLADLALESEA---ATMLMLRLAHAFDAPDDPLQRAWKRIV---TPA 382 + R +L A + + L L PD+ +++ IV TP Sbjct: 339 ADPIIVHPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPV 398 Query: 383 AKFWICKRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL 438 K ++ A T EC++V+GG+GY+ M + R+A +N I+EG+ + LD+L Sbjct: 399 VKAFLTDNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLL 454 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 103 bits (257), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 88/295 (29%), Positives = 143/295 (48%), Gaps = 40/295 (13%) Query: 178 MGMTEKQGGSDVRSNASVAMPADALQQGRGAAYLLTGHKWFFSAPMCD-----AHLMLAR 232 M +TE Q GSD+ + A+P +G G Y L G K F + D HL+LAR Sbjct: 161 MNLTEPQAGSDLALVRTRAVP-----EGDGT-YKLFGTKIFITYGEHDMAENIVHLVLAR 214 Query: 233 TEN------GLSCFFVPRWLPD-----GKRNPVLIQRLKDKLGNRSNSSSEVEFEE---A 278 T N G+S F VP++L + G RN V ++ KLG +++ ++ ++F + A Sbjct: 215 TPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGA 274 Query: 279 LGIMVGEDGRGIPTIIEMANHTRLDCVIGSTALMRQALVQAIHHARHRNAFGRL------ 332 +G +VGE+ RG+ + M N R I ++ +A +A+ +AR R L Sbjct: 275 IGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGP 334 Query: 333 --LAEQPLMRAVLADLALESEAATMLMLRLAHAFDA---PDDPLQR----AWKRIVTPAA 383 + QP +R +L + + EAA L A DA DD R A+ + P Sbjct: 335 VAIIHQPDVRRMLMSMRAQIEAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIV 394 Query: 384 KFWICKRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVL 438 K WI + +++ T ++V GG G++E A+ YR+A + I+EG+ + D++ Sbjct: 395 KGWITEMSIDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQANDLV 449 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 74/297 (24%), Positives = 134/297 (45%), Gaps = 21/297 (7%) Query: 158 SREHDGRDLPMMHKRSILIGMGMTEKQGGSDVRSNASVAMPADALQQGRGAAYLLTGHK- 216 + E + LP + + + M+E GSDV S+ + AD +G Y+L G K Sbjct: 109 NEEQKRKYLPRLISGDFIGALAMSEPNAGSDV---VSMKLRADK----KGDRYVLNGSKM 161 Query: 217 WFFSAPMCDAHLMLARTE-----NGLSCFFVPRWLPDGKRNPVLIQRLKDKLGNRSNSSS 271 W + P D ++ A+T+ G++ F V + G + + Q+L DKLG R + + Sbjct: 162 WITNGPDADVLVVYAKTDLEAGARGMTAFLVEK----GYKGFSVAQKL-DKLGMRGSHTG 216 Query: 272 EVEFEEAL---GIMVGEDGRGIPTIIEMANHTRLDCVIGSTALMRQALVQAIHHARHRNA 328 E+ F++ ++G GRG+ ++ + R G +M+ + + + R Sbjct: 217 ELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQ 276 Query: 329 FGRLLAEQPLMRAVLADLALESEAATMLMLRLAHAFDAPDDPLQRAWKRIVTPAAKFWIC 388 FG+ + E LM+ LAD+ A + + A D D + R A + Sbjct: 277 FGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSA 336 Query: 389 KRALEFTGECMEVWGGNGYVETGPMARLYREAPVNSIWEGSGNVMCLDVLRAIEHET 445 ++A GE ++ GGNGY+ P+ RL+R+A + I G+ + + + R + +T Sbjct: 337 EKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELFADT 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,742,766 Number of extensions: 73780 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 8 Length of query: 550 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 460 Effective length of database: 1,193,720 Effective search space: 549111200 Effective search space used: 549111200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)