BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0765.p (405 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 721 0.0 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 523 e-149 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 469 e-133 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 316 2e-87 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 308 5e-85 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 721 bits (1860), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/405 (85%), Positives = 369/405 (91%), Gaps = 1/405 (0%) Query: 1 MLKKIKTEDLVLGMYFCGFEAAWLDHPFWKNKFLLKHESELKQAHESGIAYCWIDPARGL 60 MLKKIK EDLVLGMYFCGFEAAWLDHPFWKNKFLLK++SEL+QAH SGIAYCWID RGL Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 61 DVAEQEPAQEQDESPASTEPEPLQE-PPEPDIDIALLDAAALRENAKKATMAMFSAARMG 119 DVA++E A + TEP P E P ID LLDA ALRE AKKAT AMFS ARMG Sbjct: 61 DVAQEEEAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEARMG 120 Query: 120 KVLDMDRCVSVVEEIAGSVVQSPNGLLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLD 179 KVLDM+RCVSVVEEIAGSV++SPNG LSVLRLKLADEYTYLHSVAVCALMVALGRTLGLD Sbjct: 121 KVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLGLD 180 Query: 180 ETACREAGLGGFLHDVGKAFLPQEVLNKPGRLTDEEFSQIKKHPELGYKYLSQDPNVPDY 239 ETACREAGLGGFLHDVGKAF+P E+LNKPG+L+DEEFSQIKKHPELGYKYLSQDPNVPDY Sbjct: 181 ETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDY 240 Query: 240 ARDVCLHHHEKMDGTGYPESLRAGNISLYSRMAAICDVYDALTSDRPYKRAWDPAEAISS 299 ARDVCLHHHEK+DG GYPE LRAG I+LYSRMAAICDVYDALTSDRPYKRAWDPAEAIS+ Sbjct: 241 ARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAISA 300 Query: 300 MATWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDN 359 MA+WTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVF Y+D+ Sbjct: 301 MASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYDDS 360 Query: 360 MEMSDTEILDLMATPEADTIIARGGEEWARLSHLDAMQLLSTGTP 404 M ++DTEILDL+A PEADTI+ARGGEEWARLSHLDAMQLLSTG P Sbjct: 361 MTVTDTEILDLLANPEADTIVARGGEEWARLSHLDAMQLLSTGVP 405 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 523 bits (1346), Expect = e-149, Method: Compositional matrix adjust. Identities = 256/395 (64%), Positives = 306/395 (77%), Gaps = 2/395 (0%) Query: 1 MLKKIKTEDLVLGMYFCGFEAAWLDHPFWKNKFLLKHESELKQAHESGIAYCWIDPARGL 60 MLKKIK DLVLGMYFCGFEA+WL HPFWK +FLL ES+L+QA SGIAYCWID +RG Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 61 DVAEQEPAQEQDESPASTEPEPLQEPPEPDIDIALLDAAALRENAKKATMAMFSAARMGK 120 DVA PA+ + + ++ + D D A A ALRE+A++ T MF+AARMGK Sbjct: 61 DVAGAAPAKAEAAAAPASPDQSAATHAGADADRA--SAVALREHAREVTRQMFNAARMGK 118 Query: 121 VLDMDRCVSVVEEIAGSVVQSPNGLLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDE 180 ++++ +CV++VEEIA SV++ PNGLLSVLRLKL DEYTYLHSV+VCAL++ LGRTLGL E Sbjct: 119 MVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSE 178 Query: 181 TACREAGLGGFLHDVGKAFLPQEVLNKPGRLTDEEFSQIKKHPELGYKYLSQDPNVPDYA 240 ACREAGL G+LHDVGKAF+ E+LNKPG+LT EEF+ IK+HPELGY+YL Q P++P YA Sbjct: 179 AACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYA 238 Query: 241 RDVCLHHHEKMDGTGYPESLRAGNISLYSRMAAICDVYDALTSDRPYKRAWDPAEAISSM 300 DVC HHHE++DG GYP++L A +I+LY+RM A+CDVYDALTS+RPYK WDPAEAIS M Sbjct: 239 SDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQM 298 Query: 301 ATWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDNM 360 A W GHFDR + LAFVKTLGIYPVGS+V+LESG SG VIRQN DLTRPVLKV Sbjct: 299 ARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPH 358 Query: 361 EMSDTEILDLMATPEADTIIARGGEEWARLSHLDA 395 E + +LDLMA P DTII RG EEW RL DA Sbjct: 359 ETTIGGVLDLMAWPRTDTIIGRGDEEWTRLRQQDA 393 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 469 bits (1208), Expect = e-133, Method: Compositional matrix adjust. Identities = 219/393 (55%), Positives = 283/393 (72%), Gaps = 9/393 (2%) Query: 2 LKKIKTEDLVLGMYFCGFEAAWLDHPFWKNKFLLKHESELKQAHESGIAYCWIDPARGLD 61 LKKI+ L +GMYF GF+ AW+D PFW+ FL++ SE+ +A +G YCWID +G+D Sbjct: 4 LKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGID 63 Query: 62 VAEQEPAQEQDESPASTEPEPL-QEPPEPDIDIALLDAAALRENAKKATMAMFSAARMGK 120 +A E D P + EPE ++D DA L ENAK+ T MF AR+GK Sbjct: 64 LA----GAEADRLPETEEPEAAGSSSSRSELD----DALRLYENAKQTTQQMFKQARLGK 115 Query: 121 VLDMDRCVSVVEEIAGSVVQSPNGLLSVLRLKLADEYTYLHSVAVCALMVALGRTLGLDE 180 LD++ C S+VEEIA SV + N LLS++RLK DEYTYLHS+AVC LMVAL RTLG+D+ Sbjct: 116 TLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDD 175 Query: 181 TACREAGLGGFLHDVGKAFLPQEVLNKPGRLTDEEFSQIKKHPELGYKYLSQDPNVPDYA 240 AC+EAGL G+LHDVGKAF+P E+LNKP RL+++E+ I++HP LGY YL VPDY Sbjct: 176 IACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYV 235 Query: 241 RDVCLHHHEKMDGTGYPESLRAGNISLYSRMAAICDVYDALTSDRPYKRAWDPAEAISSM 300 DVCLHHHEK+DG+GYP + IS+YSRMAA+CDVYDA+TS+RPYK WDPAE++S M Sbjct: 236 LDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRM 295 Query: 301 ATWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRYNDNM 360 A+W GHFDR +FLAFVK LGIYP+GSIVKL+SGR+ VIRQN DLT+PV+K F ++ Sbjct: 296 ASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSI 355 Query: 361 EMSDTEILDLMATPEADTIIARGGEEWARLSHL 393 E+ ++EI+DL A + D I R G++W + + L Sbjct: 356 EVVESEIIDLSAPGQTDAIAERSGQDWLKSNGL 388 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 316 bits (809), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 166/395 (42%), Positives = 231/395 (58%), Gaps = 8/395 (2%) Query: 1 MLKKIKTEDLVLGMYFCGFEAAWLDHPFWKNKFLLKHESELKQAHESGIAYCWIDPARGL 60 MLK I + L +GMY F AW P W+ FLL+ + +L S I ID A+G Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 61 DVAEQEPAQEQDESPA---STEPEPLQEPPEP----DIDIALLDAAALRENAKKATMAMF 113 DV E Q + + E E Q PE DI AA + +++K + MF Sbjct: 61 DVQTDEAEQGNGVESSLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLTMF 120 Query: 114 SAARMGKVLDMDRCVSVVEEIAGSVVQSPNGLLSVLRLKLADEYTYLHSVAVCALMVALG 173 S ARMGK +D+ +V EI SV ++ + ++S++RLK AD YTY+HSVAVCA+M++L Sbjct: 121 SQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLA 180 Query: 174 RTLGLDETACREAGLGGFLHDVGKAFLPQEVLNKPGRLTDEEFSQIKKHPELGYKYLSQD 233 LGL ++AG+ G LHD+GK +P +LNKP +LT+EEF ++ HP G++ L + Sbjct: 181 DQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQEL 240 Query: 234 PNVPDYARDVCLHHHEKMDGTGYPESLRAGNISLYSRMAAICDVYDALTSDRPYKRAWDP 293 P + D DV LHHHEKMDGTGYP L +IS+ +RM AICDVYDA+TSDRPYK WDP Sbjct: 241 PGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDP 300 Query: 294 AEAISSMATWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKV 353 + +I MA GHFD + AFVK +GIYPVGS V +++G V+ Q L P++++ Sbjct: 301 SRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRI 360 Query: 354 FRYNDNMEMSDTEILDLMATPEADTIIARGGEEWA 388 F Y+ N +++ T L + + A E+W Sbjct: 361 F-YSTNKKIAVTPTLIDLRRSANKIVGAVDPEKWG 394 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 308 bits (789), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 160/374 (42%), Positives = 224/374 (59%), Gaps = 4/374 (1%) Query: 1 MLKKIKTEDLVLGMYFCGFEAAWLDHPFWKNKFLLKHESELKQAHESGIAYCWIDPARGL 60 MLK I + L LGMY W+ HPF + F ++ +L+ + I I+ A+G Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 61 DVA--EQEPAQEQDESPASTEPEPLQEPPEPDIDIALLDAAALR--ENAKKATMAMFSAA 116 DV E E ++PA+ + P D A+ A R ++K + MFS A Sbjct: 61 DVTREELEEDDADQDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMFSEA 120 Query: 117 RMGKVLDMDRCVSVVEEIAGSVVQSPNGLLSVLRLKLADEYTYLHSVAVCALMVALGRTL 176 RMGK +D+ +V EI SV ++ + L+S+ RLK D+YTYLHSVAVCA+M++L L Sbjct: 121 RMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQL 180 Query: 177 GLDETACREAGLGGFLHDVGKAFLPQEVLNKPGRLTDEEFSQIKKHPELGYKYLSQDPNV 236 G+ ++AG+ G LHDVGK +P ++LNKP +LT+EEF+ ++ H G+ L Q + Sbjct: 181 GMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGI 240 Query: 237 PDYARDVCLHHHEKMDGTGYPESLRAGNISLYSRMAAICDVYDALTSDRPYKRAWDPAEA 296 + A DV LHHHEK+DG+GYP +L+A +IS+ ++M A+CDVYDA+TS+RPYK WDPA + Sbjct: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 Query: 297 ISSMATWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFRY 356 I MA GHFD AFVK +GIYP GS V L+SGR G V+ Q L P +KVF Sbjct: 301 IRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYS 360 Query: 357 NDNMEMSDTEILDL 370 N ++EI+DL Sbjct: 361 TANRMPLESEIIDL 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,230,385 Number of extensions: 48876 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 10 Length of query: 405 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 318 Effective length of database: 1,207,925 Effective search space: 384120150 Effective search space used: 384120150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)