BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0764.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 944 0.0 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 151 1e-37 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 105 6e-24 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 104 1e-23 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 88 2e-18 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 944 bits (2441), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/492 (93%), Positives = 476/492 (96%) Query: 1 MAYLLALDQGTSSSRSIIFDQSGTIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQR 60 MAYLLALDQGTSSSRSIIFDQSG IVATAQQEFPQIFPQPGWVEHDPMDLW SQLQTAQ+ Sbjct: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQK 60 Query: 61 VLAQSGIQASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 VLAQSGI+ASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE Sbjct: 61 VLAQSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 Query: 121 AIQNKTGLVLDPYFSGTKLRWILDNVAGLRERAERGELLFGTVDAWLVWNLTGGRLHVSD 180 AIQ KTGLVLDPYFSGTKLRWILD+V GLR RAERGELLFGTVD+WLVWNLTGGRLHVSD Sbjct: 121 AIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPASMLPQVHASSHLFGEIDAAHLGAAVKIGGIA 240 VSNASRTMLWNIHTGAWDEELLQWLGIPA+MLPQV S+HL+GEIDA HLGAAVKIGGIA Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVKIGGIA 240 Query: 241 GDQQSALFGQACLRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDAQKQYALE 300 GDQQSALFGQAC+RPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVD QKQYALE Sbjct: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQYALE 300 Query: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACTVEDSGGVVFVPSFTGLGAPYWNPTAKGA 360 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLAC+VEDSGGVVFVPSFTGLGAPYWNP+AKGA Sbjct: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGA 360 Query: 361 IVGLSRGSTVAHIARAALESIAFQSSALLNAMVRDAVSPITELRVDGGAAANNLLLQFQA 420 IVGLSRGSTVAHIARAALESIAFQS+ALLNAMVRDAVSPITELRVDGGAAANNLLLQFQA Sbjct: 361 IVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQFQA 420 Query: 421 DLLGIPVIRPQVTETTALGAAYLAGLATGVYRDVSELSSQWRMERRFEPGITRDEAEERI 480 DLLGIPVIRPQVTETTALGAAYLAG+ATGVYRD+SELSSQWRME FEP I RD+A+ RI Sbjct: 421 DLLGIPVIRPQVTETTALGAAYLAGIATGVYRDLSELSSQWRMEHTFEPSIGRDQAQARI 480 Query: 481 GAWEAAIRQVRA 492 AWEAAIRQV A Sbjct: 481 HAWEAAIRQVSA 492 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 151 bits (381), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 130/472 (27%), Positives = 208/472 (44%), Gaps = 28/472 (5%) Query: 3 YLLALDQGTSSSRSIIFDQSGTIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQRVL 62 Y+L +D GT+S++S++F G +VA +E+P + +PG E DP + + L + + Sbjct: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAV 68 Query: 63 AQSGIQASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAI 122 + E+ L + + + + A + + N++ W D RA V ++ G AI Sbjct: 69 KAAKAAPQEIALLSFSAAMHSVIALD-AENRLLSNSITWGDIRASVWAERIKHEHDGNAI 127 Query: 123 QNKTGLVLDPYFSGTKLRWILDNVAGLRERAERGELLFGTVDAWLVWNLTGGRLHVSDVS 182 +TG + P KL W+ + RA R F + +L++ L G V D S Sbjct: 128 YRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAAR----FVGIKEYLLYQLFGQW--VVDHS 181 Query: 183 NASRTMLWNIHTGAWDEELLQWLGIPASMLPQVHASSHLFGEID---AAHLGAAVKIGGI 239 AS T ++N+ AWD+ L LG+ LP ++H + A LG +V I Sbjct: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFI 241 Query: 240 AGDQQSAL--FGQACLRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDAQKQY 297 G L G + G T GT M D+ T G + C +K + Sbjct: 242 IGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRT-VIDEPRTDPQGRLF---CYALTEKHW 297 Query: 298 ALEGSVFIGGAVVQWLRDGLKAIKTST-------DVEGLACTVED----SGGVVFVPSFT 346 + G V GG + +W+RD L + + E L E + G++F P Sbjct: 298 VVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMA 357 Query: 347 GLGAPYWNPTAKGAIVGLSRGSTVAHIARAALESIAFQSSALLNAMVRDAVSPITELRVD 406 G AP WN +G+ GL+ H+ RAALE + F ++L A+ + V P + Sbjct: 358 GERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPAL-EELVGPTKRMMAT 416 Query: 407 GGAAANNLLLQFQADLLGIPVIRPQVTETTALGAAYLAGLATGVYRDVSELS 458 GG A + L Q AD+ V+ P+ E++ LGAA L A G+ +S +S Sbjct: 417 GGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVIS 468 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 105 bits (263), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 130/489 (26%), Positives = 214/489 (43%), Gaps = 41/489 (8%) Query: 5 LALDQGTSSSRSIIFDQSGTIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQRVLAQ 64 L +D GTS ++++ D G++VA A P P W E P D WQ+ T ++ Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 65 SGIQA-SELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAIQ 123 G + + A+G++ Q V+ + + + A++W D R+ CA L R + Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAELESR--APRLH 119 Query: 124 NKTGLVLDPYFSGTKLRWILDNVAGLRERAERGELLFGTV---DAWLVWNLTGGRLHVSD 180 G + P F+ KL W+ + L R TV WL +TG + VSD Sbjct: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMTGRK--VSD 170 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPASMLPQVHASSHLFGEI-----DAAHLGAAVK 235 S+A+ T+ ++ W +ELL G+ +P + S + G + A L + V Sbjct: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVI 230 Query: 236 IGGIAGDQQSALFGQACLRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDAQK 295 + G AGD ++ G ++PG + GT + + D+ + G A C Q+ Sbjct: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 Query: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKTSTDVEGLACTVED------SGGVVFVPSFTGL 348 + + SV + A ++W S D + L +E + +F+P +G Sbjct: 289 WHQM--SVMLSAASCLRWF-----CRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGE 341 Query: 349 GAPYWNPTAKGAIVGLSRGSTVAHIARAALESIAFQSSALLNAMVRDAVSPITELRVDGG 408 P+ +P A G GL+ A + A LE +AF L+A+ R A + + EL + GG Sbjct: 342 RTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGG 400 Query: 409 AAANNLLLQFQADLLGIPVIRPQVTET-TALGAAYLAGLATGVYRDVSELSSQWRMERRF 467 A + Q AD L + ++ +E ALGAA LA LA G D +E+ + + + Sbjct: 401 GARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG--GDEAEVCRKPAQQALY 458 Query: 468 EPGITRDEA 476 +P R A Sbjct: 459 QPDPARHAA 467 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 113/455 (24%), Positives = 194/455 (42%), Gaps = 23/455 (5%) Query: 1 MAYLLALDQGTSSSRSIIFDQSGTIVATAQQEFPQIFPQPGWVEHDPMDLWQSQLQTAQR 60 M+YL +D GTS + ++ D+ ++A+ P P W E P W + L Sbjct: 1 MSYL-GIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAA 59 Query: 61 VLAQSGIQASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 + Q+ L +G++ Q + +R G + A++W D RA C L E + + Sbjct: 60 LRTQAPQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL-EALVPD 117 Query: 121 AIQNKTGLVLDPYFSGTKLRWILDNVAGLRERAERGELLFGTVDAWLVWNLTGGRLHVSD 180 A+ TG P F+ KL W+ + ++ L +L W LTG VS+ Sbjct: 118 AVA-ITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLL----PKDFLGWKLTGE--FVSE 170 Query: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPASMLPQVHASSHLFGEIDAA-----HLGAAVK 235 +S+A+ T+ ++ W E +L G+ S +P++ S + G++ + V Sbjct: 171 MSDAAGTLWLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVV 230 Query: 236 IGGIAGDQQSALFGQACLRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDAQK 295 + G AGD ++ G +R G A + G+ + T + G+ + C Sbjct: 231 VAGGAGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWH 290 Query: 296 QYALEGSVFIGGAVVQWLRDGLKAIKTSTDVEGLA--CTVEDSGGVVFVPSFTGLGAPYW 353 Q ++ + A + WL G+ A + V G + + +F+P G P+ Sbjct: 291 QMSV---ILSAAASLHWL-SGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHN 346 Query: 354 NPTAKGAIVGLSRGSTVAHIARAALESIAFQSSALLNAMVRDAVSPITELRVDGGAAANN 413 + AKG + G++ AH+A A +E +AF + A A ++ A + + GG + + Sbjct: 347 DAAAKGVLFGMTPAHESAHLAYAVMEGVAF-AMADGYAALQAAGTTLESAAFVGGGSRSA 405 Query: 414 LLLQFQADLLGIPVIRPQVTET-TALGAAYLAGLA 447 A LGIP+ R E ALGAA L LA Sbjct: 406 FWGVLCATALGIPLRRHAGAEVGAALGAARLGRLA 440 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 129/507 (25%), Positives = 197/507 (38%), Gaps = 75/507 (14%) Query: 3 YLLALDQGTSSSRSIIFDQSGTIVATAQQEFPQIF--PQPGWVEHDPMDLWQSQLQTAQR 60 YL+ +D GT S+R+ +FD G ++A+A+ + +F + VE +W++ Q + Sbjct: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDI-DLFRDERRARVEQSSAQIWEAVCQAVRA 62 Query: 61 VLAQSGIQASELTALGITNQRETTVVWERA----TGKPVYNAVVWQDRRAEVVCAALRER 116 + +SGI + + +G+ V A G P + +VW D RA + A R Sbjct: 63 AVTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRA--LEQAQRIN 120 Query: 117 GLGEAIQNKTGLVLDPYFSGTKLRWILDNVAGLRERAERGELLFGTVDAWLVWNLTGGRL 176 +G A+ + G V+ P KL W+ +N L E F D +L W TG Sbjct: 121 RMGHAVLSYVGGVISPEMETPKLLWLKEN---LPEVYASAAHFFDLTD-FLTWKATGSLQ 176 Query: 177 HVSDVSNASRTMLWNIHTGAWDEELLQ--------------------WLGIP-ASMLPQV 215 S T L H WD + W G P A L Q Sbjct: 177 RSSCTVTCKWTYL--AHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQT 234 Query: 216 HASSHLF--------GEIDAAHLGAAVKIGGIAGDQQSA-----LFG-QAC--------- 252 A + G IDA H G + G +A +FG +C Sbjct: 235 AAQAMGLRPGIAVAAGLIDA-HAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPV 293 Query: 253 LRPGMAKNTY-GTGCFMLLHTGDQCATSHNGLISTAACQVDAQKQYALEGSVFIGGAVVQ 311 L PG+ Y M L+ G Q A G ++ A E + + Q Sbjct: 294 LVPGVWGPYYKAMAPGMWLNEGGQSAA---GAAIDHLLRLHPATPQAREEAARAQMELPQ 350 Query: 312 WL-RDGLKAIKTSTDVEGLACTVEDSGGVVFVPSFTGLGAPYWNPTAKGAIVGLSRGSTV 370 WL + L A+ ++ LA G + VP F G +P +P A+ A+VGL + Sbjct: 351 WLAQQALAAVDHPSEAVWLA------GQLTVVPEFLGNRSPLADPQARAALVGLGMEHDI 404 Query: 371 AHIAR---AALESIAFQSSALLNAMVRDAVSPITELRVDGGAAANNLLLQFQADLLGIPV 427 + A L S+ + ++ A V + + V GGA + L Q AD G+PV Sbjct: 405 DSLVALYVAGLCSLGYGLRQIIEAQAACGVR-VQSISVSGGAGVHPLTRQLLADATGMPV 463 Query: 428 IRPQVTETTALGAAYLAGLATGVYRDV 454 E LG+A LA +A G++ D+ Sbjct: 464 EITASPEPVLLGSAMLAAVAAGLHADL 490 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,521,645 Number of extensions: 63389 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 6 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)