BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0755.p (341 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 672 0.0 gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogen... 152 4e-38 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 138 6e-34 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 88 1e-18 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 87 2e-18 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 672 bits (1735), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/341 (96%), Positives = 335/341 (98%) Query: 1 MTQMMKAAVVREFGKPLSIEQVPVPTPGPGQILVKFEASGVCHTDLHAAQGDWPVKPTPP 60 MTQMMKAAVVREFGKPLSIEQVPVPTP PGQILVKFEASGVCHTDLHAA GDWPVKPTPP Sbjct: 1 MTQMMKAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPP 60 Query: 61 FIPGHEGTGYVAALGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSV 120 FIPGHEGTGYVAA+GAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSV Sbjct: 61 FIPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSV 120 Query: 121 NGTFAEYGLADPNFVGRLPASLEFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGL 180 NG+FAEYGLADP FVG LP +L+FGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGL Sbjct: 121 NGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGL 180 Query: 181 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRDHNAVADVQRIIGGAHGALV 240 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGR+HNAVA+VQRIIGGAHGALV Sbjct: 181 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHGALV 240 Query: 241 TAVSPKAMEQAFGFLRSHGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEESL 300 TAVSPKAMEQAFGFLR+ GTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEESL Sbjct: 241 TAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEESL 300 Query: 301 VFAAEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDLK 341 VFAAEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDLK Sbjct: 301 VFAAEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDLK 341 >gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 152 bits (384), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 113/335 (33%), Positives = 160/335 (47%), Gaps = 14/335 (4%) Query: 15 KPLSIEQVPVPTPGPGQILVKFEASGVCHTDLHAAQGDWPVKPTPPFIPGHEGTGYVAAL 74 KPL + G + + G+CH+DLH + +W P +PGHE G VAA+ Sbjct: 13 KPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQIRSEW-AGTQYPCVPGHEIVGRVAAV 71 Query: 75 GAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNT---------GYSVNGTFA 125 GA V K GD VGV + +C C C G E C T G+++ G Sbjct: 72 GAHVSKFKVGDVVGVGCIVDSCQSCPDCDEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQ 131 Query: 126 EYGLADPNFVGRLPASLEFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMAV 185 + + + + + AP+LCAG+T Y L+ PG+ V + GIGGLGHM + Sbjct: 132 QIVVHERYVLNIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMGI 191 Query: 186 QYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRDHNAVADVQRIIGGAHGALVTAVSP 245 + A AMG HVVA + K A AK LGAD V R+ +A + L T +P Sbjct: 192 KIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFD---FILNTVAAP 248 Query: 246 KAMEQAFGFLRSHGTMALVGLPPGDISLP-VFNTVLKRITVRGSIVGTRQDLEESLVFAA 304 ++ L+ GTM LVG P P VFN +LKR ++ GS++G + +E L F A Sbjct: 249 HDLDAFLALLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPETQEMLDFCA 308 Query: 305 EGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLD 339 E + A + D IN + RM +G + R V+D Sbjct: 309 EHGIVADIELIRADEINTAYERMLKGDVKYRFVID 343 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 138 bits (348), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 118/368 (32%), Positives = 182/368 (49%), Gaps = 45/368 (12%) Query: 5 MKAAVVREFG--------KPLSIEQVPVPTPGPGQILVKFEASGVCHTDLHAAQGDWPVK 56 ++AAV+RE +PL IE++ + PGPG++LV+ +A+G+CH+DL G V+ Sbjct: 3 IQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITG---VR 59 Query: 57 PTP-PFIPGHEGTGYVAALGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLC---AE 112 P P P GHE + V +G+GV ++ GD V + ++ +CG C PC G LC A Sbjct: 60 PRPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFV-PSCGHCLPCMEGRPALCEPGAA 118 Query: 113 QQNTGYSVNG------------------TFAEYGLADPNFVGRLPASLEFGPAAPVLCAG 154 G ++G FA++ + ++ A ++ AA CA Sbjct: 119 TNTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAV 178 Query: 155 VT-VYKGLKETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLG 212 +T V + EV+ G + G+GG+G A+ A A G V+A D+H+ KLALA+ LG Sbjct: 179 LTGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLG 238 Query: 213 ADLTVDGRDHNAVADVQRII-GGAHGALVTAVSPKAMEQAFGFLRSHGTMALVGLP-PGD 270 A TV+ RD +AV V+ + GG A A S +AME A+ R G GLP PG Sbjct: 239 ATATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQ 298 Query: 271 I-SLPVFNTVLKRITVRGSIVGT---RQDLEESLVFAAEGKVAAHFTWDK---LDNINAI 323 +L + V + TV+GS +G+ +D+ + GK+ + L +IN Sbjct: 299 HWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRG 358 Query: 324 FARMEEGK 331 F R+ G+ Sbjct: 359 FDRLHGGE 366 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 102/361 (28%), Positives = 167/361 (46%), Gaps = 39/361 (10%) Query: 6 KAAVVREFGKPLSIEQVPVPTPGPGQILVKFEASGVCHTDLHAAQGDWPVKPTPPFIPGH 65 +AAV + GKPL+IE+V + P G++LV+ +A+G+CHTD + G P + P I GH Sbjct: 4 RAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP-EGIFPAILGH 62 Query: 66 EGTGYVAALGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTG-------- 117 EG G V +G GVK +K+ D V +P C C C + LC + T Sbjct: 63 EGAGVVVDVGPGVKSLKKDDHV-IPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDA 121 Query: 118 ---YSVNG----------TFAEYGLADPNFVGRLPASLEFGPAAPVLCAGVTVYKG--LK 162 +S++G TF+ Y + + ++ + F + C GVT G L Sbjct: 122 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGC-GVTTGVGAVLF 180 Query: 163 ETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRD 221 +V G VV+ G+GG+G +Q AK +G + ++ D++ + A+A+K G ++ +D Sbjct: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 Query: 222 HNAVAD--VQRIIGGAHGALVTAVSPKAMEQAFGFL-RSHGTMALVGLPPG--DISLPVF 276 V D + GGA + + M Q+ + G ++G+ +IS F Sbjct: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 Query: 277 NTVLKRITVRGSIVG---TRQDLEESLVFAAEGKVAAHFTWDK---LDNINAIFARMEEG 330 V R+ +GS G R D+ + + + EGK+ L+ IN F M+ G Sbjct: 301 QLVTGRVW-KGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSG 359 Query: 331 K 331 + Sbjct: 360 E 360 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 84/301 (27%), Positives = 141/301 (46%), Gaps = 44/301 (14%) Query: 4 MMKAAVVREFGKPLSIEQVPVPT--PGPGQILVKFEASGVCHTDLHAAQGDWPVKPTPPF 61 M KA + + G P +E V V PG G++ ++ A G+ D + G +P +P P Sbjct: 1 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYP-QPLPAG 59 Query: 62 IPGHEGTGYVAALGAGVKHVKEGDRV---GVPWLHTACGCCSPCRTGWETLCAEQQNTGY 118 + G E G + A+GAGV K GDRV G P Sbjct: 60 L-GQEAAGTIEAVGAGVSGFKVGDRVAYAGRP---------------------------- 90 Query: 119 SVNGTFAEYGLADPNFVGRLPASLEFGPAAPVLCAGVTVYKGLKET-EVRPGEWVVI-SG 176 NG +AE + + + +LP ++ F AA ++ G+TV L++T V+PG+ ++ + Sbjct: 91 --NGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYLLRQTYAVQPGDTILFHAA 148 Query: 177 IGGLGHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRDHNAVADVQRIIGGAH 236 GG+G +A Q+A+A+G++++ ++K ALA + GA ++ + N V V+ + GG Sbjct: 149 AGGIGLIACQWARALGVNLIGTVSSDEKAALALQHGAAHVINYKTENFVERVKELTGGRG 208 Query: 237 GALV-TAVSPKAMEQAFGFLRSHGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQD 295 +V ++ + LR G M + G+ S PV + + RGS+ TR Sbjct: 209 VQVVYDSIGKDTFLGSLDCLRPRGMM----VSFGNASGPVAPFGVSELASRGSLFLTRPS 264 Query: 296 L 296 L Sbjct: 265 L 265 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,198,288 Number of extensions: 54321 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 10 Length of query: 341 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 255 Effective length of database: 1,212,660 Effective search space: 309228300 Effective search space used: 309228300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)