BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0752.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 797 0.0 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 385 e-108 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 369 e-103 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 66 5e-12 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 57 3e-09 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 797 bits (2059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/419 (93%), Positives = 406/419 (96%) Query: 1 MSAVLAPSQQQYSPASGPVPGLPRATQPAHVIRDDAEAIAVAHTLAQRLAEGASERDRLR 60 MSAVLAPS S A+ PVPGLPRA+QPAHVIRDDAEAIAVAH LA RLA+GASERD+LR Sbjct: 1 MSAVLAPSLHSTSSAAAPVPGLPRASQPAHVIRDDAEAIAVAHALAPRLAQGASERDQLR 60 Query: 61 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIIAAADPSLAQITQNHLGFVA 120 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAII+AADPSL QITQNHLGFVA Sbjct: 61 RWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVA 120 Query: 121 AVRTVSDAEQQQRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST 180 AVRTVSD EQQ+RFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST Sbjct: 121 AVRTVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDAGDHVIVTGKKFYST 180 Query: 181 GALLAHYVPIVANDEENRTWYVVADRNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKS 240 GALLAHYVPIVANDEENRTWYVVA+RNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPK+ Sbjct: 181 GALLAHYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKT 240 Query: 241 QLIPAYKGYQEPSADGAIFQIIQAAIDVGIARAAIADTQEFVRTKSRPWIDSQRDHAWED 300 LIPAYKGY EPSADGAIFQIIQAA+DVGIARAAIADTQ+FVRTKSRPWIDSQRDHAWED Sbjct: 241 HLIPAYKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWED 300 Query: 301 PYSIQAVGDLQIRLHASDALLERAGRAIDAAVAAPDAESVARAQILTAEAKVLSTEIAIH 360 PYSIQA+GDLQIRLHAS+ALLERAGRAIDAAVAAPDA+SVA AQILTAEAKVLSTEIAIH Sbjct: 301 PYSIQAIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIH 360 Query: 361 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYALIGNYYLNGVKPPFHAWS 419 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYA++GNYYLNGVKPPFHAWS Sbjct: 361 ATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 211/388 (54%), Positives = 269/388 (69%), Gaps = 2/388 (0%) Query: 32 IRDDAEAIAVAHTLAQRLAEGASERDRLRRWPVEEIEAYSQSGLWAINVPRAFGGAEVSY 91 IRDDAEAIA A LA+ + A+ RDR R+ P +E+E Y +SGLW I VP+ +GGA VSY Sbjct: 32 IRDDAEAIAAARKLAEDFSREAALRDRERKLPWDELERYVRSGLWGITVPKEYGGAGVSY 91 Query: 92 ATLAEVIAIIAAADPSLAQITQNHLGFVAAVRTVSDAEQQQRFFADFLRGVRWGNAFSES 151 TLAEVIA I+AAD S+ QI QNH V +R Q++ FF L+G R+GNA +E Sbjct: 92 GTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGSESQKRFFFERVLQGDRFGNALAEL 151 Query: 152 GSKRAADFETRFVDA--GDHVIVTGKKFYSTGALLAHYVPIVANDEENRTWYVVADRNAP 209 G+K + D +TR G + G+KFY+TGAL A +VP D++ + R+AP Sbjct: 152 GTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGALYADWVPTSVIDDDGVQQLAIVPRHAP 211 Query: 210 GLTVIDDWSAFGQRTTLSGTVIIDQVKVPKSQLIPAYKGYQEPSADGAIFQIIQAAIDVG 269 GLT+IDDWS FGQRTT SG+ + + V VP +I + P+ G + QI AAID+G Sbjct: 212 GLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRPTQIGPMAQIQHAAIDLG 271 Query: 270 IARAAIADTQEFVRTKSRPWIDSQRDHAWEDPYSIQAVGDLQIRLHASDALLERAGRAID 329 IARAA AD FVRT+SR W+DS + A +DP +I+ VGDL I+LHA++ALL RAGR +D Sbjct: 272 IARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIREVGDLTIQLHAAEALLRRAGRKVD 331 Query: 330 AAVAAPDAESVARAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNART 389 AA AAPD +SVA A I AEA+VL+TE+++ A +KLFELAG++STL ++NLDRHWRNART Sbjct: 332 AAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLAEHNLDRHWRNART 391 Query: 390 HTLHDPVRWKYALIGNYYLNGVKPPFHA 417 HTLHDPVRWKY IGNY LNG KPP H Sbjct: 392 HTLHDPVRWKYHAIGNYVLNGKKPPRHG 419 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 369 bits (948), Expect = e-103, Method: Compositional matrix adjust. Identities = 207/406 (50%), Positives = 272/406 (66%), Gaps = 4/406 (0%) Query: 18 PVPGLPRATQPAHVIRDDAEAIAVAHTLAQRLAEGASERDRLRRWPVEEIEAYSQSGLWA 77 P P +PA I ++ A+ VA LA A A++RDR R P E+IE +SQSGLWA Sbjct: 6 PEPIRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWA 65 Query: 78 INVPRAFGGAEVSYATLAEVIAIIAAADPSLAQITQNHLGFVAAVRTVSDAEQQQRFFAD 137 I VPR+ GG S+ T+AEVI +I+AADPS+ QI QNH G + + ++ EQQ+ FFA+ Sbjct: 66 ITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQRLFFAE 125 Query: 138 FLRGVRWGNAFSESGSKRAADFETRFV---DAGD-HVIVTGKKFYSTGALLAHYVPIVAN 193 L G R GNA E K D TR AGD ++ G +FYSTGAL AH++P A Sbjct: 126 VLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAI 185 Query: 194 DEENRTWYVVADRNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKSQLIPAYKGYQEPS 253 D++ + V R+APG+ VIDDWS+FGQRTT SGTV+ D V+VP +Q++ Q+P+ Sbjct: 186 DDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQPN 245 Query: 254 ADGAIFQIIQAAIDVGIARAAIADTQEFVRTKSRPWIDSQRDHAWEDPYSIQAVGDLQIR 313 G + Q+IQAAID GIA AA+ DT FV T+SRP++DS + A +DPY ++ +G+L +R Sbjct: 246 NIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLR 305 Query: 314 LHASDALLERAGRAIDAAVAAPDAESVARAQILTAEAKVLSTEIAIHATNKLFELAGTRS 373 LHA+DALLE A +D+ A E +A+A AEAKVLSTE+A+ A+ KLFEL+G+ + Sbjct: 306 LHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALLASEKLFELSGSAA 365 Query: 374 TLGQYNLDRHWRNARTHTLHDPVRWKYALIGNYYLNGVKPPFHAWS 419 TL +NLDRHWRNAR HTLHDPVRWKY +GNY LNG PP H+WS Sbjct: 366 TLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 92/382 (24%), Positives = 161/382 (42%), Gaps = 40/382 (10%) Query: 28 PAH-VIRDDAEAIAVAHTLAQRLAEGASERDRLRRWPVEEIEAYSQSGLWAINVPRAFGG 86 P H +IRD A+ H +RL A++ DR +P E ++ ++ G + VP +GG Sbjct: 5 PEHEMIRD-----AMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGG 59 Query: 87 AEVSYATLAEVIAIIAAADPSLAQI--TQNHLGFVAAVRTVSDAEQQQRFFADFLRGVRW 144 A + Y +L + IAA D + + I QN L Q++++ RG Sbjct: 60 AGMDYMSLVLALEEIAAGDGATSTIVSVQNSLA-CGITEKYGSPTQKEQWLKPLARGEML 118 Query: 145 GN-AFSE--SGSKRAADFETRFVDAGDHVIVTG-KKFYSTGALLAHYVPIVAND----EE 196 G +E +GS AA TR GD I+ G K+F ++G + D ++ Sbjct: 119 GCFCLTEPHTGSDAAA-IRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKK 177 Query: 197 NRTWYVVADRNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKSQLIPAY-KGYQEPSAD 255 + ++V + PG V GQ + + +++D +VP + L+ +GY+ ++ Sbjct: 178 GISCFLVPC-DTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSN 236 Query: 256 GAIFQIIQAAIDVGIARAAIADTQEFVRTKSR---PWIDSQRDHAWEDPYSIQAVGDLQI 312 +I AA VG+ARAA + R + P I+ Q + + D+ Sbjct: 237 LEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFR--------LADMNT 288 Query: 313 RLHASDALLERAGRAIDAAVAAPDAESVARAQILTAEAKVLSTEIAIHATNKLFELAGTR 372 L A+ ++ RA + D S+A+ A K+ S I IH G Sbjct: 289 LLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIH---------GGV 339 Query: 373 STLGQYNLDRHWRNARTHTLHD 394 + ++R +R+ R +++ Sbjct: 340 GYTSDFPVERIYRDVRICQIYE 361 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 57.0 bits (136), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 87/364 (23%), Positives = 149/364 (40%), Gaps = 41/364 (11%) Query: 46 AQRLAEGASERDRLRRWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIIAAAD 105 A +A A++ D +P++ I + G + P A GG +S V +A A Sbjct: 21 AGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATVVFEELARAC 80 Query: 106 PSLAQITQNHLGFVAAVRTVSDAEQQQRFFADFLRGVRWGN-AFSESGSKR-AADFETRF 163 S A H + + + ++ G + G+ +E GS AA +T Sbjct: 81 TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA 140 Query: 164 VDAGDHVIVTGKKFYSTGALLAHYVPIVA---NDEENRTWYVVADRNAPGLTVIDDWSAF 220 H ++ G K + +GA + ++A + +V NAPG++ S Sbjct: 141 RLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVSAIVVPGNAPGVSYGKKESKM 200 Query: 221 GQRTTLSGTVIIDQVKVPKSQL--------IPAYKGYQEPSADGAIFQIIQAAIDVGIAR 272 G + + T+ D VKVP L I A KG DG I A VG A+ Sbjct: 201 GWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGL-----DGGRINI--ATCSVGAAQ 253 Query: 273 AAIADTQEFVRTK---SRPWIDSQRDHAWEDPYSIQ-AVGDLQIRLHASDALLERAGRAI 328 AA+ +++ + RP D Q ++Q + D+Q L A+ ++ A + Sbjct: 254 AALDAAHAYMKERKQFGRPIADFQ---------ALQFKLADMQTELVAARQMVRLAATKL 304 Query: 329 DAAVAAPDAESVARAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNAR 388 DA +P+A + A AK L+T++ N+ +L G + +Y L+R++R+ R Sbjct: 305 DAG--SPEATTYC------AMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVR 356 Query: 389 THTL 392 H + Sbjct: 357 VHQI 360 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,293,116 Number of extensions: 52325 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 5 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)