BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0747.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 710 0.0 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 140 2e-34 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 109 4e-25 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 79 7e-16 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 63 5e-11 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 710 bits (1833), Expect = 0.0, Method: Compositional matrix adjust. Identities = 341/352 (96%), Positives = 348/352 (98%) Query: 32 VKFAYWVPNVSGGLVVSKIPQRTEWSLEYNQRLAVAAEKAGFEYALSQIRFTAGYGAEYQ 91 VKFAYWVPNVSGGLVVS IPQRT+WSLEYNQRLAV AEKAGFEYALSQIRFTAGYGAEYQ Sbjct: 17 VKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQ 76 Query: 92 HESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE 151 HESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE Sbjct: 77 HESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE 136 Query: 152 FQAIGEPWLEHDERYRRSHEFIQALKGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEI 211 FQAIGEPWLEHDERYRRS+EFI+ALKGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEI Sbjct: 137 FQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEI 196 Query: 212 FQGGSSRAARDNAASVSDWYFTNGNTVEALKAQIDDIQAKAAQNGHKVRIGVNAFVIARD 271 FQGGSSRAARDNAASVSDWYFTNGNT+E LKAQIDDIQAKAAQNGHKVRIGVNAFVIARD Sbjct: 197 FQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARD 256 Query: 272 TEEEAQAVLEDIIKHAHVEAVHAFGDAVKQAGKASPEGEGNWAKSSFEDLVQYNDGFKTN 331 TEEEAQAVL+DII HAHVEAVHAFGDAVKQAGKASPEGEGNWAKS+FEDLVQYNDGFKTN Sbjct: 257 TEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWAKSTFEDLVQYNDGFKTN 316 Query: 332 LIGTPQQIAERIVALKAVGVDLVLTGFLHFIEEVEYFGQKVLPLVRELEARQ 383 LIGTPQQIAERIVALKA+GVDLVLTGFLHFIEEVEYFGQKVLPLVRELEARQ Sbjct: 317 LIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLPLVRELEARQ 368 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 106/331 (32%), Positives = 167/331 (50%), Gaps = 20/331 (6%) Query: 57 SLEYNQRLAVAAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 116 +LEY +++AV +E G++ L GA V + +L+ T ++K+L A+ Sbjct: 28 TLEYMRQIAVTSETLGYDGLLIPT------GASCLDPWV-VAASLVPVTQRIKLLVALRT 80 Query: 117 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSHEFIQAL 176 P A+Q AT+D NGR+ +NVV G E +A G +L+HDERY + E++ Sbjct: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 Query: 177 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 236 + + + ++G +R N L VQ+P+P ++ GGSS AA D AA D Y T G Sbjct: 140 RKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 Query: 237 TVEALKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLEDIIKHAHVEAV---- 292 A++ +IDD++ +AA+ G VR GV I R+T EA E +I E + Sbjct: 200 PPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ 259 Query: 293 --HAFGDAVKQAGKASPEG----EGNWAKSSFEDLVQYNDGFKTNLIGTPQQIAERIVAL 346 +A D+V Q A+ G + A + + + G T+L+G +AER+ Sbjct: 260 QNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDATTVAERLQEY 319 Query: 347 KAVGVD-LVLTGFLHFIEEVEYFGQKVLPLV 376 +GVD VL+G+ H +EE F + V PL+ Sbjct: 320 ADLGVDTFVLSGYPH-LEEAIRFAELVFPLL 349 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 109 bits (272), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 102/341 (29%), Positives = 165/341 (48%), Gaps = 40/341 (11%) Query: 59 EYNQRLAVAAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGP 118 +Y +++AVAA+ G++ L G E ++ + +L+ AT +LK L A+ PG Sbjct: 28 DYMKQVAVAADTLGYDGVL----LPTGRSCE---DAWVAAASLIDATRRLKFLVAVRPGL 80 Query: 119 WHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSHEFIQALKG 178 P + A+ AT D +S GR+ INVV+G E ++ G + H ERY S EF++ + Sbjct: 81 MAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGV-FGSHAERYEISDEFLKIWRD 139 Query: 179 IWTQDDFTFKGDFYRFNNYTLSPKPVQ-------KPHPEIFQGGSSRAARDNAASVSDWY 231 + T+ T G+ F LS K + +P+P ++ GGSS A D AA D Y Sbjct: 140 VLTK---TRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGGSSDEAIDLAAEQVDVY 196 Query: 232 FTNGNTVEALKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLEDIIKHAHVEA 291 T G A+ +I ++ +AA+ G +R G+ VI R+T E A +++I E Sbjct: 197 LTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEAAWRAADELISRLDDET 256 Query: 292 VHAFGDAVKQAGKASPEGEGNWAKSSF----EDLVQYN-----------DGFKTNLIGTP 336 + A QA A + EG ++ D ++ + G T L+G Sbjct: 257 I-----AKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVRGGAGTALVGDG 311 Query: 337 QQIAERIVALKAVGVD-LVLTGFLHFIEEVEYFGQKVLPLV 376 +AER+ A+G+D +L+G+ H +EE F + V PL+ Sbjct: 312 PTVAERLQEYAALGIDTFILSGYPH-LEESYRFAELVFPLL 351 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 79.0 bits (193), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 71/307 (23%), Positives = 137/307 (44%), Gaps = 51/307 (16%) Query: 121 PAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSHEFIQALKGI 179 P + A++++T+DH+S GR+ N+V+G+ +A+G LEHD+RY R+ EF++ + + Sbjct: 113 PFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKL 172 Query: 180 WT---QDDFTFK-------------------GDFYRFNNYTLSPKPVQKPHPEIFQGGSS 217 W +D + GD+Y LS +P ++ P ++Q GSS Sbjct: 173 WEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLS-EPSRQRTPVLYQAGSS 231 Query: 218 RAARDNAASVSDWYFTNGNTVEALKAQIDDIQAKAAQNGHK---VRIGVNAFVIARDTEE 274 + A ++ F + + +A + + I+ A + G ++I + V+ TE+ Sbjct: 232 GRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQ 291 Query: 275 EAQAVLEDIIKHAHVEA--VH--------------------AFGDAVKQAGKASPEGEGN 312 +AQ + ++A EA H A +A++ K + E N Sbjct: 292 QAQERYREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELN 351 Query: 313 WAKSSFEDLVQYNDGFKTNLIGTPQQIAERIVALKA-VGVDLVLTGFLHFIEEVEYFGQK 371 W K ++ G +++G P ++A+ + + A G+D E E F + Sbjct: 352 WTKRKLLQQLELG-GRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 410 Query: 372 VLPLVRE 378 V+P+++E Sbjct: 411 VIPVLQE 417 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 57/193 (29%), Positives = 90/193 (46%), Gaps = 29/193 (15%) Query: 121 PAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSHEFIQALKGI 179 P +A++ AT+DH+S GR A NVV+ +GE G + L HD RY R+ EF++ + Sbjct: 109 PYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKL 168 Query: 180 W---TQDDFTFKGDFYRFNNYTLSPK----------PVQKP-----HPEIFQGGSSRAAR 221 W D + D Y + + P+ P HP I Q GSS + Sbjct: 169 WHSWQPDALRLQQDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQ 228 Query: 222 DNAASVSDWYFTNGNTVEALKAQIDDIQAKAAQNGHK---VRI--GVNAFVI-----ARD 271 D AA ++ F + L+A D++ +A Q G + +RI V FV AR+ Sbjct: 229 DFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARE 288 Query: 272 TEEEAQAVLEDII 284 +E+A A+ + ++ Sbjct: 289 LQEQANALADPLV 301 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,186,589 Number of extensions: 48868 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 5 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)