BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0734.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 847 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 395 e-111 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 358 e-100 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 311 9e-86 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 296 2e-81 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 847 bits (2187), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/437 (95%), Positives = 433/437 (99%) Query: 1 VQPQLSKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGF 60 +QPQL+KQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGF Sbjct: 1 MQPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGF 60 Query: 61 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML 120 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML Sbjct: 61 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML 120 Query: 121 QGFATGGEYSSSTAFLVESAPANQRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPQALD 180 QGFATGGEYSSSTAFLVESAPA++RGLYGSWQLVGQCLAVFCGAGMGALVTRNLSP+ALD Sbjct: 121 QGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALD 180 Query: 181 NWGWRLPFVIGLLIGPVGLWIRRHMHETDDFMESANKPDAAPIKLLDVVKTNLRGVLVSM 240 +WGWRLPFV+GLLIGPVGLWIRRHMHETDDF+ESANKP+AAPIKLLDVVKTNLRGVLVSM Sbjct: 181 SWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSM 240 Query: 241 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTLIIPFAGALSDRIGRR 300 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLT +IPFAGALSDR+GRR Sbjct: 241 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRR 300 Query: 301 TVLLYSSLAFLLTVYPLFVWVATAPSLPRLLVMQVLLCIMIGANYGPMPTALSEQFPTRV 360 TVLLYSSLAFLL VYPLFVWV+ APSLPRLLVMQVLLCI+IGAN+GPMPTALSEQFPTRV Sbjct: 301 TVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRV 360 Query: 361 RSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLLAACIGVAAAWAMRDQSP 420 RSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVL AACIGV+AAWAMRDQSP Sbjct: 361 RSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRDQSP 420 Query: 421 AAKNRQTEVFAGIGELK 437 AAKNRQTEVFAGIGE+K Sbjct: 421 AAKNRQTEVFAGIGEIK 437 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 395 bits (1014), Expect = e-111, Method: Compositional matrix adjust. Identities = 202/411 (49%), Positives = 278/411 (67%), Gaps = 3/411 (0%) Query: 7 KQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPVG 66 ++R ++A ++GN LEW+DF VY FF+ IIA+LFFP N+ TSLL+A+ATFG+GFFMRPVG Sbjct: 19 RRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPVG 78 Query: 67 GVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATG 126 G+LLG+YADR GRKAA+ V I+LM + +I PTY +IG+ AP++IVVAR+LQGF+ G Sbjct: 79 GILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAG 138 Query: 127 GEYSSSTAFLVESAPANQRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPQALDNWGWRL 186 GE S+TAFL E AP +R Y SW +AV GA +G VT +L AL +WGWRL Sbjct: 139 GEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRL 198 Query: 187 PFVIGLLIGPVGLWIRRHMHETDDFMESANKPDAAPIKLLDVVKTNLRGVLVSMGQVING 246 PF++G++IGPVG +IR H+ ET F+E +K D +P+K +V++ R S VI Sbjct: 199 PFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD-SPLK--EVIRNFPRETSASFSMVILW 255 Query: 247 TVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTLIIPFAGALSDRIGRRTVLLYS 306 TV YV+L MPT++ K L LP F+ M+ + ++ P G L+DRIGRR +L S Sbjct: 256 TVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSGS 315 Query: 307 SLAFLLTVYPLFVWVATAPSLPRLLVMQVLLCIMIGANYGPMPTALSEQFPTRVRSTGLA 366 + L+ YP+F ++ +APSL LL+ Q++ +I A GP+ A SE FP +V STGL+ Sbjct: 316 AALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLS 375 Query: 367 VAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLLAACIGVAAAWAMRD 417 VAYNLA +FGGFA F +TWL +GS +APA+YV++AA I +R+ Sbjct: 376 VAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVRE 426 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 358 bits (919), Expect = e-100, Method: Compositional matrix adjust. Identities = 176/415 (42%), Positives = 278/415 (66%), Gaps = 7/415 (1%) Query: 2 QPQLSKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFF 61 PQ +R +I++ +GNALEW+D ++YG F+ +IA+ FFP ++ SLL+ ATFG+ FF Sbjct: 14 HPQ--ARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFF 71 Query: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 MRP+G V+LG Y+DR GRKAA+ + I+LMT+ A+IAF P+YA+IG+ AP I + +M+Q Sbjct: 72 MRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQ 131 Query: 122 GFATGGEYSSSTAFLVESAPANQRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPQALDN 181 GF+ GGE+ SSTAFLVE AP ++RG + SWQ+ Q +++ A GA++ L+P+ L + Sbjct: 132 GFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLAS 190 Query: 182 WGWRLPFVIGLLIGPVGLWIRRHMHETDDFMESANKPDAAPIKLLDVVKTNLRGVLVSMG 241 WGWR+PF+ GLLI P G++IRRH+ E +F ES+ K + AP++ D +L+ G Sbjct: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTN-APLR--DTFAHQKMRLLIGAG 247 Query: 242 QVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTLIIPFAGALSDRIGRRT 301 VI TV+ Y+ L +PT+A KQLGLP +F +A ++ + P GALSD+IGR Sbjct: 248 SVIMATVSVYLSLY-IPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTP 306 Query: 302 VLLYSSLAFLLTVYPLFVWVATAPSLPRLLVMQVLLCIMIGANYGPMPTALSEQFPTRVR 361 ++ SS +++ YP+FV++ +P +LL++Q ++ +++ + MP L++ FP R Sbjct: 307 FMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATR 366 Query: 362 STGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLLAACIGVAAAWAMR 416 TG+++AYN+A +FGGFA I+TWL +G ++ +++V+ A + + A+ + R Sbjct: 367 GTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 311 bits (796), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 168/421 (39%), Positives = 247/421 (58%), Gaps = 5/421 (1%) Query: 4 QLSKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMR 63 + SK V+ GN LE +DF +YGF+++ I++ FFP+DNE+ SL++ TFG GF MR Sbjct: 9 KHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMR 68 Query: 64 PVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGF 123 P+G ++LG Y DR GR+ + V + +M + +IAF P YATIG+AAPI+++ R+LQGF Sbjct: 69 PLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGF 128 Query: 124 ATGGEYSSSTAFLVESAPANQRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPQALDNWG 183 + G E + +L E A +G Y SWQ Q A+ A +G LV+++LSP + +WG Sbjct: 129 SAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWG 188 Query: 184 WRLPFVIGLLIGPVGLWIRRHMHETDDFMESANKPDAAPIKLLDVVKTNLRGVLVSMGQV 243 WR+PF IG +I P IRR + ETD+F+ P I + + N + V+ M V Sbjct: 189 WRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEI--MSSIAQNWKIVVAGMMMV 246 Query: 244 INGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTLI-IPFAGALSDRIGRRTV 302 + TV+FY++ V PTF L L + +V V L +P GALSDR+GRR V Sbjct: 247 VMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTF-CVGLSNFCWLPIMGALSDRVGRRPV 305 Query: 303 LLYSSLAFLLTVYPLFVWVATAPSLPRLLVMQVLLCIMIGANYGPMPTALSEQFPTRVRS 362 L+ +L LLT YP+ W+ + S LL+ ++ L + + G AL+E P VR+ Sbjct: 306 LVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRT 365 Query: 363 TGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLLAACIGVAAAWAM-RDQSPA 421 G ++AY+LA +FGGF P + TWL +TSG AP +++ AA G+ A + R +S A Sbjct: 366 VGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKSAA 425 Query: 422 A 422 A Sbjct: 426 A 426 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 296 bits (759), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 156/407 (38%), Positives = 240/407 (58%), Gaps = 4/407 (0%) Query: 6 SKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 SK V+ GN +E +DF ++GF++ I++ FFP+DNE+ SL++ TFG GF MRP+ Sbjct: 9 SKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPL 68 Query: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 G ++LG Y DR GR+ + V + LM L +IAF P+YATIG AP+++++ R+LQGF+ Sbjct: 69 GAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSA 128 Query: 126 GGEYSSSTAFLVESAPANQRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPQALDNWGWR 185 G E + +L E A +G Y SWQ Q +A+ A +G L++ +L+P + WGWR Sbjct: 129 GVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWR 188 Query: 186 LPFVIGLLIGPVGLWIRRHMHETDDFMESANKPDAAPIKLLDVVKTNLRGVLVSMGQVIN 245 +PF IG LI PV IRR + ET++FM ++P ++ + N + V+ M V Sbjct: 189 IPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTG--QIFRSMIENWKLVIAGMMLVSM 246 Query: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTLI-IPFAGALSDRIGRRTVLL 304 TV+FY++ V PTF L L + + +V + V L I +P GALSDR+GRR +L+ Sbjct: 247 TTVSFYLITVYTPTFGKNVLKLSTEASLIVTL-CVGLSNFIWLPIMGALSDRVGRRPLLV 305 Query: 305 YSSLAFLLTVYPLFVWVATAPSLPRLLVMQVLLCIMIGANYGPMPTALSEQFPTRVRSTG 364 ++ +LT YP W+ S ++L++++ L + + G M AL+E P VR+ G Sbjct: 306 TFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAG 365 Query: 365 LAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLLAACIGVAA 411 ++AY+LA +FGGF P I T L + + AP ++ AA G+ A Sbjct: 366 FSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIA 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,437,228 Number of extensions: 64001 Number of successful extensions: 417 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 384 Number of HSP's successfully gapped: 15 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)