BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0686.p
         (446 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioaden...   894   0.0  
gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase ...   144   1e-35

>gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioadenine
           synthetase [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  894 bits (2311), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 434/446 (97%), Positives = 439/446 (98%)

Query: 1   MQKKVFIKTFGCQMNEYDSDKMADVLNVAEGLIKTDRPEEADVILLNTCSIREKAQEKVF 60
           MQKKVFIKTFGCQMNEYDSDKMADVLN ++GLIKTDRPEEADVILLNTCSIREKAQEKVF
Sbjct: 1   MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60

Query: 61  SDLGRLRALKKDKPDLLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120
           SDLGRLRALKK+ P LLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY
Sbjct: 61  SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120

Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180
           SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED
Sbjct: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180

Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGTMEDGEIADFALLIEYIAELPGIERIRFVTSH 240
           VLTEVAGLADQGVKEIMLLGQNVNAYRG MEDGEIADFALLIEYIAELPGIERIRFVTSH
Sbjct: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240

Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300
           PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI
Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300

Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAANLQDDTPAEVKLA 360
           TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFS RPGTPAANL DDTP EVKLA
Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360

Query: 361 RLQRLQAALNDNAAKISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420
           RLQRLQAALNDNAA+ISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR
Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420

Query: 421 LIGQIIEVSITEAFPFSLRGQIVTQH 446
           LIGQIIEVSITEAFPFSLRG+IVTQH
Sbjct: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446


>gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 458

 Score =  144 bits (364), Expect = 1e-35,   Method: Compositional matrix adjust.
 Identities = 133/461 (28%), Positives = 214/461 (46%), Gaps = 43/461 (9%)

Query: 4   KVFIKTFGCQMNEYDSDKMADVLNVAEGLIKTDRPEEADVILLNTCSIREKAQEKVFSDL 63
           KV   + GC     DS+++   L  AEG       + AD++++NTC   + A ++    +
Sbjct: 14  KVGFVSLGCPKALVDSEQILTQLR-AEGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAI 72

Query: 64  GRLRALKKDKPDLLIGVGGCVASQEGEAIIKR-APFVDMVFGPQTLHRLPQMISERRYSG 122
           G   AL ++   ++ G  G     EG+ II++  P V  V GP  +  + Q + +     
Sbjct: 73  GE--ALNENGKVIVTGCLGAKKDAEGDDIIQKIHPKVLEVTGPHAVGEVMQAVHKH---- 126

Query: 123 RPQVDISFPEIEKFDHLPPARVEGAT---AYVSIMEGCSKYCSYCVVPYTRGEEVSRRFE 179
                +  P     D +PP  V+      AY+ I EGC+  CS+C++P  RG+ VSR   
Sbjct: 127 -----LPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIA 181

Query: 180 DVLTEVAGLADQGVKEIMLLGQNVNAY------RGTMEDGEIADFAL--LIEYIAELP-- 229
           DV+ E   L   GVKE++++ Q+ +AY      R    +G+     +  L+  + EL   
Sbjct: 182 DVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPVKTHMTQLVGALGELAQQ 241

Query: 230 --GIERIRFVTSHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYK 287
                R+ +V  +P     ++I   A+  K+  +L +P QH     L  MKR     +  
Sbjct: 242 YGAWVRLHYVYPYPH--VDQIIPMMAE-GKILPYLDVPLQHAHPDVLKRMKRPANGEKNI 298

Query: 288 SVIRRMRKVRPDITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAA 347
             I+  R + PD+T++S FI GFPGETE +FE L+  + +   D    F +SP  G  A 
Sbjct: 299 ERIQAWRAMCPDLTIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATAN 358

Query: 348 NLQDDTPAEVKLARLQRLQAALNDNAAKISAAQVGTVQRVLVEGVSKRREHELQGRTESN 407
            + +  P EV+  R  R+     + + K   A+VG   RVL++ V +       GRT S+
Sbjct: 359 EIANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGG---TGRTYSD 415

Query: 408 RVVNFDG--------GPNGQRLIGQIIEVSITEAFPFSLRG 440
                DG         P+ +  +GQ ++V IT A    L G
Sbjct: 416 -APEIDGVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWG 455


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,305,819
Number of extensions: 53226
Number of successful extensions: 166
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 164
Number of HSP's successfully gapped: 2
Length of query: 446
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 358
Effective length of database: 1,203,190
Effective search space: 430742020
Effective search space used: 430742020
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)