BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0683.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 573 e-165 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 103 2e-23 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 77 2e-15 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 75 9e-15 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 573 bits (1478), Expect = e-165, Method: Compositional matrix adjust. Identities = 284/292 (97%), Positives = 289/292 (98%) Query: 1 MQDHPSSVKSDAKPHRSLFERLTALISPEPENRAELLEVLHDAHERNLIDADALSMIEGV 60 MQDHPSSVKSDAKPHRSLFERLTALISPEPENRAELLEVLHDAHERNLIDADALSMIEGV Sbjct: 1 MQDHPSSVKSDAKPHRSLFERLTALISPEPENRAELLEVLHDAHERNLIDADALSMIEGV 60 Query: 61 FQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAIDGERDKVIGILLAKD 120 FQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPA+DGERDKV+GILLAKD Sbjct: 61 FQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVGILLAKD 120 Query: 121 LLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGVAGLITIED 180 LLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGVAGLITIED Sbjct: 121 LLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGVAGLITIED 180 Query: 181 VLEQIVGDIEDEYDFDEAEDNVLALKDGPNGACWRVKALTEIEQFNEEVGSRFSDEDADT 240 VLEQIVGDIEDEYDFDEAEDNVLALKDGP+GA WRVKALTEIEQFNEEVGSRFSD+DADT Sbjct: 181 VLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNEEVGSRFSDDDADT 240 Query: 241 IGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLPPTPVADE 292 IGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLPP P +DE Sbjct: 241 IGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLPPAPASDE 292 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 55/229 (24%), Positives = 119/229 (51%), Gaps = 9/229 (3%) Query: 55 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAIDGERDKVIG 114 S++ +F + ++V D+M PRSQ++ ++++ P++ + ++ H++ P +GE + ++G Sbjct: 194 SILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGEINHIVG 253 Query: 115 ILLAKDLLRYYAEESF----DVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYG 170 IL + + + E D+R++L ++P + L+ F+ N + VVVDEYG Sbjct: 254 ILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGVVVDEYG 313 Query: 171 GVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPNGACWRVKALTEIEQFNEEVG 230 V GL+T+ED++E+++G+ E +GAC ++ T + N+ +G Sbjct: 314 EVQGLVTLEDIIEEMIGEFTTSSPGSSGE----GFGWDKDGACL-LEGSTTLRDINKRLG 368 Query: 231 SRFSDEDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVL 279 + + TI GL+ ++ +P +DG E+++ + + ++ Sbjct: 369 LQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMV 417 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 67/249 (26%), Positives = 119/249 (47%), Gaps = 21/249 (8%) Query: 52 DALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAIDGERDK 111 D +++ ++L V DIM P S++ + K +E + + +SR+P D ER + Sbjct: 205 DEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYYDAERQQ 264 Query: 112 VIGILLAKDLLRYYAEES----FDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVD 167 V+G++ KD+ + A++ +++D LRP +I + LLR FR H AV+ Sbjct: 265 VLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPALPALDLLRRFRTGSPHFAVIGK 322 Query: 168 EYGGVAGLITIEDVLEQIVGDIEDEYDFDEAE----DNVLALKDGPNGACWRVKALTEIE 223 + AG IT++++L +VG+I DE+ + E D+ L G + L + Sbjct: 323 KGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGTLLGKG----SLPIVTLENML 378 Query: 224 QFNEEVGSRFSDEDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQ 283 + E D+ D++GGLV +G +P++G + D F+V+ ++LV Sbjct: 379 GIDIEY-----DDSIDSVGGLVMEKLGDLPKEGQKIDFPA--FDVVIKRMSGPKIVLVKV 431 Query: 284 LPPTPVADE 292 P A E Sbjct: 432 YPKLQEARE 440 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 74.7 bits (182), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 63/245 (25%), Positives = 114/245 (46%), Gaps = 34/245 (13%) Query: 56 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAIDGERDKVIGI 115 +IE VF++ V M PR + ++ T +++ HSRF DG D V+G Sbjct: 202 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSRFLVCDGHIDAVLGY 261 Query: 116 LLAKDLL-RYYAEESFDVRD--MLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGV 172 + +KD+L R E F + D ++ P + IP+S L +L +A +A+VV+EY V Sbjct: 262 IDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 321 Query: 173 AGLITIEDVLEQIVGDI----EDEYDFDEAEDNVLALKDGPNGACWRVKALT-------- 220 G+I+ DV ++G++ E+E ++N W + LT Sbjct: 322 VGIISTTDVTGVLMGNLLAITEEEQQIVRRDEN-----------SWLMDGLTPLNDVMSE 370 Query: 221 -EIEQFNEEVGSRFSDEDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVL 279 +I+++ VG +T+ G + + ++P+K D FEV+ + +++ L Sbjct: 371 LDIDEYPNPVGY-------ETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRINQL 423 Query: 280 LVSQL 284 LV++ Sbjct: 424 LVTRF 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 919,289 Number of extensions: 38565 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 4 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)