BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0615.p (328 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 598 e-172 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 146 2e-36 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 141 7e-35 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 115 6e-27 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 108 4e-25 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 598 bits (1542), Expect = e-172, Method: Compositional matrix adjust. Identities = 305/328 (92%), Positives = 315/328 (96%) Query: 1 MQRRHFLRLAPAALGATLLGSTPLIAGAAQPPKTLRLDYAYYSPPSLVLRKFGWLEEDLK 60 M RR FLRLAPAALG T LG PL++ AAQPPK +RLDYAYYSPPSLVLRKFGWLEEDLK Sbjct: 1 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 61 AQGTEVKWVLSQGSNRALEYLNSDSIDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 AQGTEVKWVLSQGSNRALEYLNSDS+DFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 Query: 121 AVGKDSPVRSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 AVGK+SPV+SI+ELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE Sbjct: 121 AVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 Query: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARYPDQVKRVLAAYE 240 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAAR+PDQVKRV+AAYE Sbjct: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE 240 Query: 241 RARHWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSHPQIGREHIEALRPAAPILLEE 300 RAR WIIAHPDETVALLSEESKLSAPVARLQLKRNDFS PQIGREHIEALR AAPILLEE Sbjct: 241 RARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE 300 Query: 301 DLVKSSTDLPRTINALIDPSYAQDVVKA 328 DLVKS TDLPRTINALIDPSYAQ VV+A Sbjct: 301 DLVKSGTDLPRTINALIDPSYAQGVVRA 328 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 146 bits (369), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 102/277 (36%), Positives = 147/277 (53%), Gaps = 7/277 (2%) Query: 2 QRRHFLRLAPAALGA--TLLGSTPLIAGAAQPPKTLRLDYAYYSPPSLVLRKFGWLEEDL 59 +RR RL+ ALGA LG++P+ AQ K LR+ Y + ++L+ G LE+ L Sbjct: 13 RRRAIQRLSAIALGALTIALGASPV---QAQETKLLRIGYQKAANTLVLLKAHGTLEKRL 69 Query: 60 KAQGTEVKWVLSQGSNRALEYLNSDSIDFGSTAGLAALLARANG-NPVKAVYIYSRPEWT 118 + G EVKW + LE LN S+DFG + A+A G + V Y P+ Sbjct: 70 QPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAE 129 Query: 119 ALAVGKDSPVRSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAA 178 L V K+SP++S++ELKGKKIA KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 130 GLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAA 189 Query: 179 LEQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARYPDQVKRVLA 237 E+G V AWA DP AA+E + G+R + V N + F + FAA+ P K +L Sbjct: 190 FERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILLE 249 Query: 238 AYERARHWIIAHPDETVALLSEESKLSAPVARLQLKR 274 R W+ H + A L+ L A + LKR Sbjct: 250 EISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKR 286 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 141 bits (356), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 104/310 (33%), Positives = 159/310 (51%), Gaps = 11/310 (3%) Query: 2 QRRHFLRLAPAALGATLLGS-TPLIAGAAQPPKTLRLDYAYYSPPSLVLRKFGWLEEDLK 60 Q RH RL L A L S TPL+ AA K LR+ Y ++ + +L+ G LE+ L+ Sbjct: 5 QPRHKRRLLLQGLAAGALASGTPLVRAAAD--KVLRIGYQKFNTLN-ILKGSGKLEQALQ 61 Query: 61 AQGTEVKWVLSQGSNRALEYLNSDSIDFGSTAGLAALLARANGNPVKAVYIYSR---PEW 117 QG VKW + E LN+++ID G A ++ A A+G V AVY+ + P+ Sbjct: 62 PQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASG--VNAVYLGAEQPYPKG 119 Query: 118 TALAVGKDSPVRSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQH-ADGR 176 + V ++SP+RS+++LKGKK+A +G + LL+AL E GL D+E V++ + AD R Sbjct: 120 IGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVR 179 Query: 177 AALEQGRVAAWAGLDPHLAASELEAGSRLIYRNVNF-NTYGFLNVSDSFAARYPDQVKRV 235 AA + G V A DP LA EL R++ N F SF R+ D V+ V Sbjct: 180 AAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTV 239 Query: 236 LAAYERARHWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSHPQIGREHIEALRPAAP 295 A ++ W +HP ET LL+ + + V RL +R +++ + + + A Sbjct: 240 FAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIAD 299 Query: 296 ILLEEDLVKS 305 L+ L+K+ Sbjct: 300 AFLKLGLIKN 309 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 115 bits (287), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 103/320 (32%), Positives = 146/320 (45%), Gaps = 22/320 (6%) Query: 3 RRHFLRLAPA-ALGATLLGSTPLIAGAAQPPKTLRLDYAYYSPPSLVLRKFGWLEEDLKA 61 RR L A A LG+ L ++ AQ K +R+ Y S +L+ G LE+ L Sbjct: 10 RRRLLHAATALGLGSALAPASVF----AQGNKAIRIGYQKSSTLLTILKANGTLEKLLAP 65 Query: 62 QGTEVKWVLSQGSNRALEYLNSDSIDFGS-TAGLAALLARANGNPVKAVYIYS-RPEWTA 119 G ++ W LE LN +D + A + A+A G + V S P A Sbjct: 66 TGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQA 125 Query: 120 LAVGKDSPVRSISELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAAL 179 + V DS +R S+LKGKKIA TK + L+ L + GLK SDV+ +L ADGRAA Sbjct: 126 IVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAAF 185 Query: 180 EQGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYG-FLNVSDSFAARYPDQVKRVLAA 238 E+G V AW DP LA + ++ R++ Y + S SFA P+ ++ V Sbjct: 186 ERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFDE 245 Query: 239 YERARHWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSHPQIGREHIEALRPAAPILL 298 ++ HW+ HP E A L+ L A L R + + A+RP A L Sbjct: 246 LQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSY--------EVRAVRPEA--LG 295 Query: 299 EE----DLVKSSTDLPRTIN 314 E+ D ++ LPR IN Sbjct: 296 EQQRIADTFFAAGLLPRKIN 315 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 108 bits (271), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 93/293 (31%), Positives = 143/293 (48%), Gaps = 16/293 (5%) Query: 29 AQPPKTLRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEVKWVLSQGSNRALEYLNSDSIDF 88 A P+ LR+ Y ++R G LE+ L G VKWV LE L + SIDF Sbjct: 47 AAAPRQLRV--GYQKGALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDF 104 Query: 89 GSTAGLAALLARANGNPVKAVYIYS---RPEWTALAVGKDSPVRSISELKGKKIAATKGT 145 G + A+A G P+ VY+ + RP A+ V DS ++ +LKGK+IA KG+ Sbjct: 105 GDVGEAPPIFAQAAGTPL--VYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGS 162 Query: 146 DPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRVAAWAGLDPHLAASELEAGSRL 205 + + FL++ L ++GLK DV V L AD RAA E+G V AWA +P+LAA++ +R+ Sbjct: 163 NVHYFLVKLLEKHGLKYGDVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARI 222 Query: 206 I--YRNVNFNTYGFLNVSDSFAARYPDQVKRVLAAYERARHWIIAHPDETVALLSEESKL 263 + V N + + D +A + D ++ L + W A PDE + L+ L Sbjct: 223 LADADGVVKNRAYYFSTRD-YARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGL 281 Query: 264 SAPVARLQLKRNDFSHPQIGREHIEALRPAAPILLEEDLVKSSTDLPRTINAL 316 V + ++R + I R ++ + A L+ L+ PR I+ L Sbjct: 282 PRSVLDVVVRRYHYGTLPITRAILDEQQQIADTFLDLRLI------PRKISLL 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 948,985 Number of extensions: 37339 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 7 Length of query: 328 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 242 Effective length of database: 1,212,660 Effective search space: 293463720 Effective search space used: 293463720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)