BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0604.p
         (218 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb...   442   e-126
gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb...   172   2e-44

>gi|300309973|ref|YP_003774065.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 218

 Score =  442 bits (1138), Expect = e-126,   Method: Compositional matrix adjust.
 Identities = 212/218 (97%), Positives = 216/218 (99%)

Query: 1   MPKTTLRITSKNYSSWSLRGWLMAKFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH 60
           MPKTTLRITSKNYSSWSLRGWLMA+FAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH
Sbjct: 1   MPKTTLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH 60

Query: 61  HGNVLVWDTLAIAEYLNEIRPGAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG 120
           HG+VLVWDTLAIAEYLNEIRP AKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG
Sbjct: 61  HGDVLVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG 120

Query: 121 HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRTMADAMYAPVVTRFMTYDVKL 180
           HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRT+ADAMYAPVVTRFMTYDVKL
Sbjct: 121 HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRTVADAMYAPVVTRFMTYDVKL 180

Query: 181 DPICTAYAKRIMAMPEMQQWIMAAKKEPEDIDELDVEF 218
           DPICTAYAKRIMAMPEMQ+WI AAKKEPEDIDELDVEF
Sbjct: 181 DPICTAYAKRIMAMPEMQEWITAAKKEPEDIDELDVEF 218


>gi|300313568|ref|YP_003777660.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 233

 Score =  172 bits (436), Expect = 2e-44,   Method: Compositional matrix adjust.
 Identities = 92/206 (44%), Positives = 127/206 (61%), Gaps = 2/206 (0%)

Query: 5   TLRITSKNYSSWSLRGWLMAKFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGNV 64
           TL I +KNYSSWS+R W+      + F+E  +  D     AEI   + S  VP L  G++
Sbjct: 23  TLVIANKNYSSWSMRPWVAMVAFDIPFKEVRLLLDQADTAAEIARYTGSGRVPVLLDGDI 82

Query: 65  LVWDTLAIAEYLNEIRPGAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKGHFPN 124
            +WD+L+I EYL E  P   L PA   ARA  RS+C EMHSGF+ LR+A+ M+++G +P 
Sbjct: 83  AIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAEMHSGFTGLRSAMSMDIRGRYPG 142

Query: 125 FKVWSRAEADIVRITTIWKECLAKYG-GPFLFGEQRTMADAMYAPVVTRFMTYDVKLDPI 183
               + A+ADI R+  IW+ECL+++G   FLFG+  ++ADA YAPVV RF++Y V L P 
Sbjct: 143 QGRTAEAQADIARVCEIWEECLSQFGHHDFLFGD-FSIADAFYAPVVMRFISYQVPLAPA 201

Query: 184 CTAYAKRIMAMPEMQQWIMAAKKEPE 209
             AYA R+   P + +WI  A  E E
Sbjct: 202 LQAYADRVREHPAVARWIAEALAEKE 227


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.135    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 675,611
Number of extensions: 24006
Number of successful extensions: 58
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 3
Length of query: 218
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 136
Effective length of database: 1,231,600
Effective search space: 167497600
Effective search space used: 167497600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 128 (53.9 bits)