BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0592.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309962|ref|YP_003774054.1| response regulator [Herbaspiril... 728 0.0 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 192 4e-50 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 174 1e-44 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 174 1e-44 gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364... 171 1e-43 >gi|300309962|ref|YP_003774054.1| response regulator [Herbaspirillum seropedicae SmR1] Length = 420 Score = 728 bits (1879), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/417 (88%), Positives = 394/417 (94%) Query: 1 MPLENLNELIESALVVDDSALQRQHSVALLQELGVDLIYEAGNGNEALDLLAMLKLPPGL 60 MP +NLNELI+SALVVDDSA+QRQHSV LLQ+LG DLIYEAGNGNEALDLL +LKLPP L Sbjct: 1 MPADNLNELIKSALVVDDSAVQRQHSVQLLQQLGADLIYEAGNGNEALDLLGLLKLPPSL 60 Query: 61 VVIDLEMPGMDGIELIQQLQQKGIDIPLIVASSRETSLLLSVETMIQALGMNLIGVLQKP 120 +V+DLEMPGMDGIELIQQLQQKGID+PLIVASSRETSLLLSVETMI ALGMNLIGVLQKP Sbjct: 61 MVVDLEMPGMDGIELIQQLQQKGIDVPLIVASSRETSLLLSVETMIHALGMNLIGVLQKP 120 Query: 121 LNQGQLRAALQAFRPHGAAARAHDDPLPSMCESDLGAAIGGGQLLPYFQPKVDVQTGILK 180 LNQ QLRAAL++FRPHG + DP PSMCE DLGAAI GGQ+LPYFQPKVDVQTGILK Sbjct: 121 LNQHQLRAALESFRPHGTEHQPDHDPQPSMCEHDLGAAIEGGQVLPYFQPKVDVQTGILK 180 Query: 181 GVEALARWIHPERGMVPPDRFVPLAEQCGLIHDLTISMMDQSMAQAAVWHSRGLAIKVAV 240 GVEALARWIHPERGMV PDRF+PLAEQCGLIH LT++MMDQ+M QAA+WHSRGLAIKVAV Sbjct: 181 GVEALARWIHPERGMVSPDRFIPLAEQCGLIHPLTLAMMDQAMGQAAIWHSRGLAIKVAV 240 Query: 241 NLSPLSLEQPDFMHRILALVDKHALPAENVVLEITESSVVAHKGNSLGMLARLRLKGFGL 300 NLSPL LE+PDF+HRIL LVDKHALPAE+VVLEITESSVVAHKGNSLGMLARLRLKGFGL Sbjct: 241 NLSPLLLEKPDFLHRILELVDKHALPAEHVVLEITESSVVAHKGNSLGMLARLRLKGFGL 300 Query: 301 SIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHDRKNLRVILQSALDMAQRLELVTVAE 360 SIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHDRKNLRVILQSALDMAQRLELVTVAE Sbjct: 301 SIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHDRKNLRVILQSALDMAQRLELVTVAE 360 Query: 361 GVETMEDWRLLQDFGCSLGQGFLIGKPMPANELPLWLKGHHRRLNELRPLGKGAATG 417 GVETMEDWRLLQDFGCSLGQG+LIGKPMPA+ELPLWLKGHHRRL+ELRP+ +G A+G Sbjct: 361 GVETMEDWRLLQDFGCSLGQGYLIGKPMPAHELPLWLKGHHRRLHELRPVARGTASG 417 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 192 bits (488), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 97/234 (41%), Positives = 138/234 (58%) Query: 163 QLLPYFQPKVDVQTGILKGVEALARWIHPERGMVPPDRFVPLAEQCGLIHDLTISMMDQS 222 + + Y QPK+++ +G + G EAL RWIHPE+G + PD F+P AE G I LT+ MM+Q Sbjct: 546 EFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWMMEQV 605 Query: 223 MAQAAVWHSRGLAIKVAVNLSPLSLEQPDFMHRILALVDKHALPAENVVLEITESSVVAH 282 + +G+ +K +VNLS L + A++ +H L + LEITES+++ Sbjct: 606 ARLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITESAIMDD 665 Query: 283 KGNSLGMLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHDRKNLRVI 342 + L RL GF LSIDD+GTG+SS+ L R+P ELKID+SFV ++ I Sbjct: 666 PARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKI 725 Query: 343 LQSALDMAQRLELVTVAEGVETMEDWRLLQDFGCSLGQGFLIGKPMPANELPLW 396 ++S +D+ L L VAEGVE+ E W LL+ GC GQG+ +GKPMP W Sbjct: 726 VRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLAW 779 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 174 bits (442), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 91/251 (36%), Positives = 148/251 (58%), Gaps = 1/251 (0%) Query: 152 ESDLGAAIGGGQLLPYFQPKVDVQTGILKGVEALARWIHPERGMVPPDRFVPLAEQCGLI 211 ES L A+ GG L FQP++ + L GVEALARW HP+ G + P RF+P+AE+CGLI Sbjct: 733 ESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIPIAEECGLI 792 Query: 212 HDLTISMMDQSMAQAAVWHSRGLAI-KVAVNLSPLSLEQPDFMHRILALVDKHALPAENV 270 +L ++++ Q + W RG+ + ++VNLS + D + L+ ++AL ++ Sbjct: 793 GELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFVTGLLRRYALEPSDL 852 Query: 271 VLEITESSVVAHKGNSLGMLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFV 330 LEITE+ ++ ++ +A + G L++DD+GTG+SS+ L ++P +ELK+D+SFV Sbjct: 853 TLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFV 912 Query: 331 HGAHDRKNLRVILQSALDMAQRLELVTVAEGVETMEDWRLLQDFGCSLGQGFLIGKPMPA 390 + + + + + + L+L VAEGVE E + LL GC + QG+L +PM A Sbjct: 913 RDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRPMSA 972 Query: 391 NELPLWLKGHH 401 + L W++ H Sbjct: 973 DALENWVRDLH 983 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 174 bits (441), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 101/248 (40%), Positives = 146/248 (58%), Gaps = 1/248 (0%) Query: 152 ESDLGAAIGGGQLLPYFQPKVDVQTGILKGVEALARWIHPERGMVPPDRFVPLAEQCGLI 211 E DL A+ G+ L ++QPKVD+ +G + G EAL RW HP+ GMV P RF+ LAE+ GLI Sbjct: 695 EGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLI 754 Query: 212 HDLTISMMDQSMAQAAVWHSRGLA-IKVAVNLSPLSLEQPDFMHRILALVDKHALPAENV 270 + ++ + Q W G A I V+VNLS Q D + I +++ LP E++ Sbjct: 755 LPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESL 814 Query: 271 VLEITESSVVAHKGNSLGMLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFV 330 LE+TES ++ +++G+LA L+ G LSIDD+GTG+SS+ L R P LKIDRSFV Sbjct: 815 ELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFV 874 Query: 331 HGAHDRKNLRVILQSALDMAQRLELVTVAEGVETMEDWRLLQDFGCSLGQGFLIGKPMPA 390 + I S + +A+ L+L +AEGVET E L+ +GC QG+L P+PA Sbjct: 875 RDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPA 934 Query: 391 NELPLWLK 398 ++ L+ Sbjct: 935 DKFEQILR 942 >gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364 [Herbaspirillum seropedicae SmR1] Length = 606 Score = 171 bits (433), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 91/242 (37%), Positives = 139/242 (57%) Query: 156 GAAIGGGQLLPYFQPKVDVQTGILKGVEALARWIHPERGMVPPDRFVPLAEQCGLIHDLT 215 A + QL FQPKV + TG GVEAL RW HP G++ P F+PLAE+ LI +T Sbjct: 361 AALLSTDQLWLEFQPKVSLSTGRCAGVEALLRWRHPIHGVISPALFIPLAEKTALIRQVT 420 Query: 216 ISMMDQSMAQAAVWHSRGLAIKVAVNLSPLSLEQPDFMHRILALVDKHALPAENVVLEIT 275 + ++ + + QA W RG+AI VA+N+S + D + + L+++H +PA + +E T Sbjct: 421 LWVLQRGIEQATAWARRGVAIPVAINVSARDFDNDDLLQLLGRLLEQHRIPAGLIEIEFT 480 Query: 276 ESSVVAHKGNSLGMLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHD 335 ES++ + G + ++ G ++IDD+G GFS++ L IP LK+D+SF+ Sbjct: 481 ESAMSRNPEKLYGRIQEIKRLGVKVAIDDFGAGFSNLSYLKHIPADYLKLDQSFILRMES 540 Query: 336 RKNLRVILQSALDMAQRLELVTVAEGVETMEDWRLLQDFGCSLGQGFLIGKPMPANELPL 395 + + I+ S + + QRL VAEGVE + R+LQ GC GQGF I +PM A + Sbjct: 541 DYSDQQIVPSMIHLGQRLGFAVVAEGVENEKTRRILQHLGCDFGQGFGIARPMNAADCWD 600 Query: 396 WL 397 W+ Sbjct: 601 WI 602 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,395,964 Number of extensions: 62093 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 19 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)