BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0591.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 565 e-162 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 237 1e-63 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 165 7e-42 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 142 4e-35 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 86 5e-18 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 565 bits (1456), Expect = e-162, Method: Compositional matrix adjust. Identities = 298/368 (80%), Positives = 318/368 (86%), Gaps = 5/368 (1%) Query: 10 RQLVQQLLAQTTDIRVIGTAPDPVFALRKMQQEWPDVILLDIEMPRMDGITFLRQIMSTR 69 RQL+QQ+LAQT DIRVIGTAPDPVFALRKMQQ+WPDVI+LDI+MPRMDG++FLRQIM+TR Sbjct: 19 RQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATR 78 Query: 70 PTPVIMCSARAMGQGGLAAEALAAGAVSVIARPQLGVREFLHEAVAELAGAIRAAMLAGP 129 PTPVIMCSA LAAEALAAGAVSVIARPQLGVR+FL +AV ELA AIRAAM A Sbjct: 79 PTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQARE 138 Query: 130 GGRGTTMVTRSVPLTDDLRMVKAASREMLHDDGWASGLQRYTADAVLTPPGPQDGYCPPT 189 G R P L VKA +REMLHD W + LQRYTADAVL PPGP+DG P T Sbjct: 139 GQRAP-----KAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPAT 193 Query: 190 ARIVAIGASTGGPQALERVLGRLDGRCPGIVVVQHMPQRFTRSFAERLHRVSGAEVKEAE 249 ARIVAIGASTGGPQALERVLG+L RCPGIVVVQHMPQRFTRSFAERLHR+SGAEVKEAE Sbjct: 194 ARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRISGAEVKEAE 253 Query: 250 HQDLVLPGRVLIAPGGKHLVVRRDGQQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGRNA 309 H D +LPGRVLIAPGG+HLVVRR+G QYYVETLDGPLVSRHKPSVDVLFRSLAKAAG NA Sbjct: 254 HLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNA 313 Query: 310 VGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPKEAIHMGGVGDVLPLEHISA 369 VGIIMTGMGDDGARGMRE+ADCG ATYAQDEASSVVFGMPKEAI GGVGDV+ LEHISA Sbjct: 314 VGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHISA 373 Query: 370 VIEQYASR 377 VIEQYA R Sbjct: 374 VIEQYACR 381 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 237 bits (605), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 145/393 (36%), Positives = 211/393 (53%), Gaps = 59/393 (15%) Query: 1 MVVDDSSVVRQLVQQLLAQTTDIRVIGTAPDPVFALRKMQQEWPDVILLDIEMPRMDGIT 60 ++VDDS+++R ++++++ D+ V+G APDP+ A ++Q PDV+ LD+EMPRMDG+ Sbjct: 6 LIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLD 65 Query: 61 FLRQIMSTRPTPVIMCSARAMGQGGLAAEALAAGAVSVIARPQLGVREFLHEAVAELAGA 120 FL ++M RP PV+M S+ + AL GA+ + +P++ ++ + E +A Sbjct: 66 FLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADK 125 Query: 121 IRAAMLA------------GPGGRGTTMVTRSVPLTDDLRMVKAASREMLHDDGWASGLQ 168 IRAA A GG + PLT S E L G ++G Sbjct: 126 IRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLT---------SSEKLIIIGASTGGT 176 Query: 169 RYTADAVLTPPGPQDGYCPPTARIVAIGASTGGPQALERVLGRLDGRCPGIVVVQHMPQR 228 + ++ P CP GI++ QHMP+ Sbjct: 177 EAIKEFLIRMPSD----CP------------------------------GILITQHMPEG 202 Query: 229 FTRSFAERLHRVSGAEVKEAEHQDLVLPGRVLIAPGGKHLVVRRDGQQYYVETLDGPLVS 288 FTRSFA+RL+ + V E+ + VLPG IAPG HL + R G Y + GP V+ Sbjct: 203 FTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVN 262 Query: 289 RHKPSVDVLFRSLAKAAGRNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGM 348 RH+PSVDVLF+S A AG+NAVG+I+TGMG DGA+GM EM + GA +AQDEAS VVFGM Sbjct: 263 RHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGM 322 Query: 349 PKEAIHMGGVGDVLPLE----HISAVIEQYASR 377 P+EAI +G +VL L+ + A + ++ SR Sbjct: 323 PREAIAVGAAHEVLALQAMPGRVLAWLAEHGSR 355 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 165 bits (417), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 134/383 (34%), Positives = 184/383 (48%), Gaps = 46/383 (12%) Query: 1 MVVDDSSVVRQLVQQLLAQT--TDIRVIGTAPDPVFALRKMQQEWPDVILLDIEMPRMDG 58 +++DDS+++R+ + Q+ Q DI + + V R PDV+ LDI MP MDG Sbjct: 5 LIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFA---PDVVTLDINMPEMDG 61 Query: 59 ITFLRQIMSTRPTPVIMCSARAMGQGGLAA-EALAAGAVSVIARPQLGVREFLHEAVAEL 117 ++ L +M RP PV+M S+ QG LA EA+ GAV +A+P + LHE +L Sbjct: 62 LSALSLMMLDRPVPVVMVSSLT-EQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 118 AGAIRAAMLAGPGGRGTT--MVTRSVPLTDDLRMVKAASREMLHDDGWASGLQRYTADAV 175 +R A A R + PL + +AA R DG Sbjct: 121 LAKVRVAAKARIRSRAPAPELARAREPLAAPV--AEAARRGRPASDG------------- 165 Query: 176 LTPPGPQDGYCPPTARIVAIGASTGGPQALERVLGRLDGRCPG-IVVVQHMPQRFTRSFA 234 +V IG STGGP LE +L L P I+V QHMP FTRSFA Sbjct: 166 ----------------LVLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFA 209 Query: 235 ERLHRVSGAEVKEAEHQDLVLPGRVLIAPGGKHLVVRRDGQQYYVETLDGPLVSRH--KP 292 ER+ ++ V E +V G V IA GG ++V + G V L P H P Sbjct: 210 ERMDKLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRGSHLSV--LPKPESPTHLWHP 267 Query: 293 SVDVLFRS-LAKAAGRNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPKE 351 SV++L RS L A R +G+++TGMG+DGA E+ G T A+ E+S VVFGMPK+ Sbjct: 268 SVELLARSALQSMAARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKD 327 Query: 352 AIHMGGVGDVLPLEHISAVIEQY 374 I GG VLP I+ + + Sbjct: 328 LIDRGGASVVLPASKIATQLRVW 350 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 112/380 (29%), Positives = 185/380 (48%), Gaps = 55/380 (14%) Query: 2 VVDDSSVVRQLVQQLLAQTTDIRVIGTAPDPVFALRKMQQEWPDVILLDIEMPRMDGITF 61 +V+D+ ++ +++++++A++ +I A D A++ + PDV+L+D+ MP++DG Sbjct: 5 IVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVDGAEA 64 Query: 62 LRQIMSTRPTPVIMCSARAMGQGGLAAEALAAGAVSVIARPQL----GVREF--LHEAVA 115 R+IM P P+++ ++ EA+ GA+ P L G R+ L E + Sbjct: 65 TRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALIEKID 124 Query: 116 ELAGAIRAAMLAGP--GGRGTTMVTRSVPLTDDLRMVKAASREMLHDDGWASGLQRYTAD 173 L G + M++ P GGR T+ D L Sbjct: 125 NL-GRLSPEMVSPPVRGGRAETLAPAQPARADSL-------------------------- 157 Query: 174 AVLTPPGPQDGYCPPTARIVAIGASTGGPQALERVLGRL-DGRCPGIVVVQHMPQRFTRS 232 PP +VA+GAS GGP AL +L + G +V+VQH+ F Sbjct: 158 -------------PP---LVAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPG 201 Query: 233 FAERLHRVSGAEVKEAEHQDLVLPGRVLIAPGGKHLVVRRDGQQYYVETLDGPLVSRHKP 292 A+ L + S +V A+ G VL+A +HLVV+ G+ YVE G ++P Sbjct: 202 MAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVKEGGRLGYVE---GAPNDVYRP 258 Query: 293 SVDVLFRSLAKAAGRNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPKEA 352 SVDV F+S+ + G+++TGMG DGA G++ + D GA T AQD+ S V+GMPK A Sbjct: 259 SVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAA 318 Query: 353 IHMGGVGDVLPLEHISAVIE 372 + + +VLP+ ++A ++ Sbjct: 319 VALNAAVEVLPVTGMAARLQ 338 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 61/184 (33%), Positives = 94/184 (51%), Gaps = 9/184 (4%) Query: 193 VAIGASTGGPQALERVLGRLD-GRCPGIVVVQHMPQR----FTRSFAERLHRVSGAEVKE 247 + IGAS GG +AL +L L G +VV H+P+ + FA+R + V E Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQ----PVVE 72 Query: 248 AEHQDLVLPGRVLIAPGGKHLVVRRDGQQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGR 307 A+ ++ G + AP HL+V R + + LV+ +P++DVLF S A G Sbjct: 73 AQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGP 132 Query: 308 NAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPKEAIHMGGVGDVLPLEHI 367 +GI++TG DGA GM+ +A G T Q + V MP +A+ MG + VL L+ I Sbjct: 133 RLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQI 192 Query: 368 SAVI 371 + ++ Sbjct: 193 ADLL 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,234,347 Number of extensions: 55118 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 10 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)