BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0590.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 426 e-120 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 141 7e-35 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 135 5e-33 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 77 2e-15 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 72 8e-14 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 426 bits (1094), Expect = e-120, Method: Compositional matrix adjust. Identities = 214/275 (77%), Positives = 228/275 (82%), Gaps = 3/275 (1%) Query: 27 VGAHG---GDEISDHDMLLFQQLFKRHIGLHLPLSKKALLQSRLNHRLHELGLRDLTQYF 83 G HG +E+SDHDMLLFQ+LFK+ IGLHLPLSKKALLQSRL RL+ELGLR L QY Sbjct: 2 TGPHGIALDEEVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYH 61 Query: 84 RRISGEHDVEERQRAIDLITTNETYFFREQGHFDFLKQELLPQWEQHKSLRVWSAACATG 143 IS E +ERQRAIDLITTNETYFFRE GHFDF+ + LPQ Q LR+WSAA ATG Sbjct: 62 ALISSEAGAQERQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASATG 121 Query: 144 EEAYTLAMLLDQQRAAGSWSVLGSDISLRVLRFARRALYPMARGQNIPRPYLRRYCLLGT 203 EEAYTLAMLLD R SWSV SDIS RVLRFARRALYPM RGQNIPR YLRRYCL+G Sbjct: 122 EEAYTLAMLLDHHRPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGV 181 Query: 204 GEYEGHFLVQSDLRAKVDFAQRNLTALHEHDDGLYDLIFLRNVIIYFDSDTKLKVLRDVI 263 GEYEGHFLVQ+DLRAKV FAQRNLT L EHDDG YDLIFLRNVIIYFD +TKL+VLRDVI Sbjct: 182 GEYEGHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVI 241 Query: 264 ARLRPGGYLVVGHSESLHGMELELLMHSPSIYRKP 298 ARLRPGGYLVVGHSESLHGM+L L M SPSIYRKP Sbjct: 242 ARLRPGGYLVVGHSESLHGMDLGLDMQSPSIYRKP 276 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 141 bits (355), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 92/278 (33%), Positives = 138/278 (49%), Gaps = 19/278 (6%) Query: 28 GAHGGDEI--SDHDMLLFQQLFKRHIGLHLPLSKKALLQSRLNHRLHELGLRDLTQYFRR 85 G G E + D + L + G+ L SK+ ++ SRL RL G+ QY Sbjct: 9 GESSGKEFDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDM 68 Query: 86 ISGEHDVEERQRAIDLITTNETYFFREQGHF----DFLKQELLPQWEQHKSLRVWSAACA 141 + HD EE + + +TTN T FFRE HF DFLK P +W +A + Sbjct: 69 LERTHDSEEWEAFTNALTTNLTSFFREAHHFPILADFLKTLKEPA-------TIWCSAAS 121 Query: 142 TGEEAYTLAMLLDQQRAAGSWS----VLGSDISLRVLRFARRALYPMARGQNIPRPYLRR 197 TGEE YT+AM + A GS + ++ +DI VL +YPM R + + +++ Sbjct: 122 TGEEPYTIAMTACE--AYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKK 179 Query: 198 YCLLGTGEYEGHFLVQSDLRAKVDFAQRNLTALHEHDDGLYDLIFLRNVIIYFDSDTKLK 257 + L G G +EG+ V+ +LR + F Q NL A G +D+IF RNV+IYFD T+ K Sbjct: 180 FFLRGKGSHEGYVRVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSK 239 Query: 258 VLRDVIARLRPGGYLVVGHSESLHGMELELLMHSPSIY 295 +L + ++P L GHSE+ + L + ++Y Sbjct: 240 ILSRFVPLMKPHALLFAGHSENFLYVSDALKLKGKTVY 277 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 135 bits (340), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 85/256 (33%), Positives = 131/256 (51%), Gaps = 16/256 (6%) Query: 35 ISDHDMLLFQQLFKRHIGLHLPLSKKALLQSRLNHRLHELGLRDLTQYFRRISGEHDVEE 94 IS+ D L F++ F R G+ +K+ + RL R+ G YF + E D +E Sbjct: 6 ISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKE 65 Query: 95 RQRAIDLITTNETYFFREQGHFDFLKQELLPQWEQHK----SLRVWSAACATGEEAYTLA 150 Q +L+T NETYF RE+ F L +LP+ +++ +R+W C++GEEAYTLA Sbjct: 66 LQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLA 125 Query: 151 M-LLDQQRAAGSWSV--LGSDISLRVLRFARRALYPMARGQNIPRPYLRRYCLLGTGEYE 207 M L+++ W V + SDI VLR A+ Y Q++P ++ +Y L EY+ Sbjct: 126 MYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ 185 Query: 208 GHFLVQSDLRAKVDFAQRNLTALHEHDDGLY---DLIFLRNVIIYFDSDTKLKVLRDVIA 264 + LR V+F + NL D Y D+IF RN++IYFD ++ + Sbjct: 186 ----ICDALRGAVEFTRVNLA--KSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYE 239 Query: 265 RLRPGGYLVVGHSESL 280 L G++ +GHSES+ Sbjct: 240 ALNLNGFICLGHSESM 255 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 66/206 (32%), Positives = 101/206 (49%), Gaps = 16/206 (7%) Query: 96 QRAIDLITTNETYFFREQGHFDFLKQELLPQWEQHKS--LRVWSAACATGEEAYTLAM-L 152 Q I+L ET+FFR++ + + + + +R+ S C+TGEE Y+LAM L Sbjct: 58 QALIELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMAL 117 Query: 153 LDQQRAAGSWSVLGSDISLRVLRFARRALYPMA--RGQNIPRPYLRRYCLLGTGEYEGHF 210 LD G + + DI R L A LY RGQ + + R+ L G++ H Sbjct: 118 LDAGLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELA--FRDRHFDLEQGQWRLH- 174 Query: 211 LVQSDLRAKVDFAQRNLTA--LHEHDDGLYDLIFLRNVIIYFDSDTKLKVLRDVIARLRP 268 +R++V FA NL A H YD IF RNV+IYF+ D + +V++ + + ++P Sbjct: 175 ---ERIRSQVRFAPGNLLAPDFLRHSQP-YDFIFCRNVLIYFERDVQRQVVQLLASVMKP 230 Query: 269 GGYLVVGHSESLHGMELELLMHSPSI 294 + VG +E G+ L M S I Sbjct: 231 DALIFVGPAEG--GILLGPTMESAGI 254 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 71.6 bits (174), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 61/249 (24%), Positives = 112/249 (44%), Gaps = 18/249 (7%) Query: 61 ALLQSRLNHRLHELGLRDLTQYFRRISGEHDVEERQRAIDLITTNETYFFREQGHFDFLK 120 A L+ RL + + L+Q R+ HD ++ +T + FR+ +F L+ Sbjct: 39 ASLKRRLATAMIRFDCQTLSQLQDRVL--HDAAVFPLLMEYLTVQVSEMFRDPSYFLALR 96 Query: 121 QELLPQWEQHKSLRVWSAACATGEEAYTLAMLLDQQRAAGSWSVLGSDISLRVLRFARRA 180 ++++P + SL++W A C++GEE Y+LA+LL ++ + +DI+ L+ A Sbjct: 97 EKVVPLLRTYPSLKIWVAGCSSGEEVYSLAILLREEGLLERSIIYATDINQSALKKAEAG 156 Query: 181 LYPMARGQNIPRPYLRRYCLLGTGEY----EGHFLVQSDLRAKVDFAQRNLTALHEHDDG 236 +Y + R + + +Y G + LR V F+ +L D Sbjct: 157 VYDLERIAGFTNNHQKSGARTSLSDYYTAAYGAVVFDKSLRKNVVFSDHSLAT-----DS 211 Query: 237 LYD---LIFLRNVIIYFDSDTKLKVLRDVIARLRPGGYLVVGHSESL----HGMELELLM 289 ++ L+ RNV+IYF+ + + L L G+L +G ESL H + E ++ Sbjct: 212 VFAEMHLVSCRNVLIYFNRQLQERALGLFRESLVRKGFLGLGSKESLRFSSHAVAFEEVV 271 Query: 290 HSPSIYRKP 298 I++K Sbjct: 272 REDRIFQKK 280 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 891,397 Number of extensions: 35318 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 5 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)