BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0587.p (731 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 1159 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 321 1e-88 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 245 1e-65 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 235 7e-63 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 177 3e-45 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 1159 bits (2997), Expect = 0.0, Method: Compositional matrix adjust. Identities = 594/732 (81%), Positives = 645/732 (88%), Gaps = 10/732 (1%) Query: 1 MKKDSARDAVVQEARELLVAMESALLQIEVEGPSRDRINAIFRAAHTIKGSAGLFAFDSI 60 M KDSAR A+VQEARELLVAME+ALLQ E+EGPSRD I+AIFRAAHTIKGSAGLFAFDSI Sbjct: 1 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI 60 Query: 61 VQFTHQVEHVLDLVREERLALNGPLMSLLLQCGDYIGELVDAIERGQEAEEPNAQRRAAL 120 VQFTH +EHVL+ VREERLAL+ LMSLLLQC DYIGELVDAIERGQEAEEPNA+RRAAL Sbjct: 61 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAAL 120 Query: 121 LAELEQVALSAPAQAVTRVDPQMAVAVAVQGPSATLAPELEASAQLSVDCPYWHLSLQFN 180 LAEL+ VA + V +A A++G + + E + Q + PYWHLSLQ N Sbjct: 121 LAELDAVAAFSVDHGPASVPVPLA---ALRGAAGS---ESATADQAATRVPYWHLSLQLN 174 Query: 181 QNVLRDGLDPLSFLHYLRSLGRIVAILPVESSIPEAAAMDAESCYLGVEICLASDSTRQT 240 +NVLRDGLDPLSFLHYLRSLGRIVA++ VES+IPEAAAMDAESCYLG+E+CLASDST QT Sbjct: 175 ENVLRDGLDPLSFLHYLRSLGRIVALVTVESAIPEAAAMDAESCYLGMEVCLASDSTLQT 234 Query: 241 LEDVFEFVRDDSRIDILPPHSRLADYATVLQRLAPEEAVRLASLWRGLGLFDDAQWRCLG 300 LE+VFEFVRDDSRIDILPP + L+ YA L RLAP++A RLA+ WRGL LFD QW+ L Sbjct: 235 LENVFEFVRDDSRIDILPPRAPLSAYAERLARLAPQDAARLAAAWRGLALFDQTQWQSLE 294 Query: 301 AIGL--KDSAAPPLLSSAIADVVQRPRPLEERRAQEQKFIKIEVSKLDQLIDLVGELVIA 358 A D+ A + A V+QRPR L+ERRAQEQKFIKIEVSKLDQLIDLVGELVIA Sbjct: 295 AASATPSDALAAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGELVIA 354 Query: 359 GASARLIARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDL 418 GASARLIARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDL Sbjct: 355 GASARLIARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDL 414 Query: 419 DKDIELSMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERIAAGKPAKATLRLNA 478 KDI+L MTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAER+AAGKPAKA+L+LNA Sbjct: 415 GKDIDLVMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNA 474 Query: 479 MHESGSVVVEVMDDGRGMDRDRILEKAVAQGLVAPDAELTDAEIYRCVFEPGFSTAQQVT 538 +HESGSVV+EVMDDGRGMDR+ IL KAVAQGL +PDAEL+DAEIYR VFEPGFSTA+QVT Sbjct: 475 IHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVT 534 Query: 539 ELSGRGVGMDVVKRNIDALHGEVAIDTERGRGTVVRLRLPLTLAIISGFQVVVGNAVFVI 598 ELSGRGVGMDVV+RNI+ALHGEV+IDT RGRGTVVRLRLPLTLAIISGFQVVVGNAVFVI Sbjct: 535 ELSGRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVI 594 Query: 599 PLDMVVECIDMPPQHNGTHIVSVRGEPLPFVPLRELFDLPAREQGRARKSLVIVQYGQLR 658 PLDMVVECIDMPPQ G+HIV+VR EPLPFVPLRELFDLPARE+G R SLVIVQYG LR Sbjct: 595 PLDMVVECIDMPPQQPGSHIVNVRDEPLPFVPLRELFDLPARERG--RNSLVIVQYGPLR 652 Query: 659 AGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIPHLVHHTQQQEQGN 718 AGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDI HLVHHTQQQEQG+ Sbjct: 653 AGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQQEQGS 712 Query: 719 TAAYAERGVASA 730 AYAERGVASA Sbjct: 713 AQAYAERGVASA 724 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 321 bits (823), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 171/390 (43%), Positives = 251/390 (64%), Gaps = 9/390 (2%) Query: 332 AQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDSQFEEATQAIDTLMEQIRD- 390 E I++ V K+DQLI+LVGELVI A + E ++ L RD Sbjct: 301 GHESSSIRVSVEKVDQLINLVGELVITQAMIEQRVGKLDPMTHEHLLNSVSQLSRNTRDL 360 Query: 391 --AALTLRMVQINEVFQRFPRIVRDIARDLDKDIELSMTGSDTELDKSMVERLADPLMHI 448 + +++RM+ ++ VF RFPR+VRD+A L K +E G+ TELDK ++ER+ DPL H+ Sbjct: 361 QESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHL 420 Query: 449 VRNAIDHGIEPAAERIAAGKPAKATLRLNAMHESGSVVVEVMDDGRGMDRDRILEKAVAQ 508 VRN+IDHGIE R A+GK TL L+A H+ G++++EV DDG G++R++IL KA Q Sbjct: 421 VRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQ 480 Query: 509 GLVAPDAELTDAEIYRCVFEPGFSTAQQVTELSGRGVGMDVVKRNIDALHGEVAIDTERG 568 G+ D L+DAE+++ +FEPGFSTA+QVT++SGRGVGMDVVKRNI A+ G V I + +G Sbjct: 481 GMPVTD-NLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKG 539 Query: 569 RGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECI-----DMPPQHNGTHIVSVRG 623 GT + + LPLTLAI+ G + +G V+++PL VVE + D+ ++ VRG Sbjct: 540 FGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVLRVRG 599 Query: 624 EPLPFVPLRELFDLPAREQGRARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGK 683 E LP +PL ++F++ ++ +VI++ +A L VD L+G+ Q V+K L + K Sbjct: 600 EYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLESNYRK 659 Query: 684 VKGLSGSTILGDGRVALILDIPHLVHHTQQ 713 V G+SG+TILGDG VALI+D+ L+ ++Q Sbjct: 660 VAGISGATILGDGGVALIVDVAALLRSSRQ 689 Score = 63.9 bits (154), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 34/106 (32%), Positives = 55/106 (51%) Query: 21 MESALLQIEVEGPSRDRINAIFRAAHTIKGSAGLFAFDSIVQFTHQVEHVLDLVREERLA 80 ME LL + V P + +NA+FRAAH+IKG + F I + TH +E +LD +R+ ++A Sbjct: 1 MEKLLLAVNVASPDPEDLNAVFRAAHSIKGGSSTFGLTDIAEVTHVLESLLDRIRQGQMA 60 Query: 81 LNGPLMSLLLQCGDYIGELVDAIERGQEAEEPNAQRRAALLAELEQ 126 L + L D + ++D + G + ++ LL L Q Sbjct: 61 LTSEHVDAFLAAKDVLKSMLDGHKHGADVDQGAVADVRRLLEALSQ 106 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 245 bits (625), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 138/394 (35%), Positives = 243/394 (61%), Gaps = 20/394 (5%) Query: 330 RRAQE----QKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDS--------QFEEA 377 RRA+E K +K+E K+D+L++L+GE+V++ + +A+R +D + + Sbjct: 439 RRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIKAQ 498 Query: 378 TQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLDKDIELSMTGSDTELDKSM 437 I+ + E+++DA + +RM+ ++ VFQRFPR+VRDI+R L K+++L + G +TE DK++ Sbjct: 499 YAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNI 558 Query: 438 VERLADPLMHIVRNAIDHGIEPAAERIAAGKPAKATLRLNAMHESGSVVVEVMDDGRGMD 497 +E LADPL+HIVRN++DHG+E +R A+GKPA +L ++A HE VV+ + DDG+G+D Sbjct: 559 IEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGID 618 Query: 498 RDRILEKAVAQGLV--APDAELTDAEIYRCVFEPGFSTAQQVTELSGRGVGMDVVKRNID 555 I KA +GL+ A ++D + VF GFSTA+ V++LSGRGVGMDVV+ ++ Sbjct: 619 PALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVE 678 Query: 556 ALHGEVAIDTERGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQH-- 613 ++G +A+ + G+GT +++ LPL++A+ + +F +P++ V+E + +P Sbjct: 679 KVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLH 738 Query: 614 --NGTHIVSVRGEPLPFVPLRELFDLPAREQGRA--RKSLVIVQYGQLRAGLLVDGLLGE 669 N + + +RG L + ++ + A ++ A ++++V+ G GL++D Sbjct: 739 FANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRET 798 Query: 670 CQAVIKPLGRLFGKVKGLSGSTILGDGRVALILD 703 + KPL + G + SGS ++GDG V ++L+ Sbjct: 799 VDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLN 832 Score = 67.0 bits (162), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 37/121 (30%), Positives = 64/121 (52%) Query: 7 RDAVVQEARELLVAMESALLQIEVEGPSRDRINAIFRAAHTIKGSAGLFAFDSIVQFTHQ 66 RD + E R+LL + L+Q+E + + ++FR HT+KG++GLF + + H Sbjct: 5 RDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVLHA 64 Query: 67 VEHVLDLVREERLALNGPLMSLLLQCGDYIGELVDAIERGQEAEEPNAQRRAALLAELEQ 126 E ++D VR ++A + L LL D++ L D +E E +P+AQ + + L Q Sbjct: 65 CEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIASSLRQ 124 Query: 127 V 127 + Sbjct: 125 L 125 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 235 bits (600), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 141/397 (35%), Positives = 231/397 (58%), Gaps = 23/397 (5%) Query: 334 EQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDSQFEEATQAI-DTLME------ 386 +++ I+I+ KLD L+++ GE V A A ++ + + +FE A+ TL E Sbjct: 247 KEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAAALMSTLAETLGRAS 306 Query: 387 ----QIRDAALTLRMVQINEVFQRFPRIVRDIARDLDKDIELSMTGSDTELDKSMVERLA 442 +++ A L+ RM + +FQ+FPR+VR++A+DL KD++L +TG++TE+D+ +V+ L Sbjct: 307 RYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGAETEVDRVVVDSLY 366 Query: 443 DPLMHIVRNAIDHGIEPAAERIAAGKPAKATLRLNAMHESGSVVVEVMDDGRGMDRDRIL 502 DPL+H++RN++DHGIE +R+AAGKP KAT+ LNA SV++EV DDG+GMD D + Sbjct: 367 DPLVHMLRNSLDHGIE--VDRVAAGKPTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLR 424 Query: 503 EKAVAQGLVAPDAELTDAEIYRCVFEPGFSTAQQVTELSGRGVGMDVVKRNIDALHGEVA 562 KA+A+GL+ LT E + VF PGFST + + +SGRGVGMDVVK ++ G + Sbjct: 425 SKALAKGLINEHQALTADEALQLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIR 484 Query: 563 IDTERGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQHN---GTHIV 619 I++ G+GT +RLP+ L+II V A +P+ +V +++P + G ++ Sbjct: 485 IESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMAVVERVVELPENFDEVGGAPVL 544 Query: 620 SVRGEPLPFVPLRELFDLPAREQGRARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGR 679 +G PL P+ L + E G R +V+ G V+ + G VIKPL Sbjct: 545 RDQGRPL---PVNSLAGVLGYEPGNERVGIVMAVPHPYILG--VESVEGTADLVIKPLTA 599 Query: 680 LFGKVKGLSGSTILGDGRVALILDIPHLVHHTQQQEQ 716 + G++G+ +G + L++ + L+ + E+ Sbjct: 600 I--HTAGITGTARSAEGELVLVVGLSFLLDGCRDMER 634 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 177 bits (449), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 113/315 (35%), Positives = 176/315 (55%), Gaps = 9/315 (2%) Query: 396 RMVQINEVFQRFPRIVRDIARDLDKDIELSMTGSDTELDKSMVERLADPLMHIVRNAIDH 455 RMV+ N + R +VR +A + K + L ++ ++D++++ERL PL H++RNA H Sbjct: 412 RMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILERLMGPLEHLLRNAAVH 471 Query: 456 GIEPAAERIAAGKPAKATLRLNAMHESGSVVVEVMDDGRGMDRDRILEKAVAQGLVAPDA 515 GIE A +R+A GKPA L + A HE V+ V DDGRG+D + I +KA+ G A A Sbjct: 472 GIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEAIRQKALELG-AAGLA 530 Query: 516 ELTDAEIYRCVFEPGFSTAQQVTELSGRGVGMDVVKRNIDALHGEVAIDTERGRGTVVRL 575 E + A + +F+PG ST+ QV+ L+GRG+GMDVV+ + AL G + + ++ G+G L Sbjct: 531 EASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGWMRVHSQPGQGVQFTL 590 Query: 576 RLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDM-PPQHN---GTHIVSVRGEPLPFVPL 631 LPL+LA+ + G F IP +V + + PQ + +G L P Sbjct: 591 YLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALAERSLEHQGSRLALHPW 650 Query: 632 RELFD---LPAREQGRARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLS 688 L D PA E + L+I + L A +L D + G + V+KP+G + G+ Sbjct: 651 HCLLDEAPEPAGEGSDSAYVLLIKRDDGLMA-VLADHVSGNREVVVKPVGPQLATLAGVV 709 Query: 689 GSTILGDGRVALILD 703 G+T+LGDG++ LI++ Sbjct: 710 GATVLGDGQIVLIIN 724 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,085,674 Number of extensions: 86233 Number of successful extensions: 433 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 417 Number of HSP's successfully gapped: 10 Length of query: 731 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 639 Effective length of database: 1,184,250 Effective search space: 756735750 Effective search space used: 756735750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)